{"id":28,"date":"2022-03-21T11:50:56","date_gmt":"2022-03-21T04:50:56","guid":{"rendered":"http:\/\/sibenzyme.com\/?page_id=28"},"modified":"2026-09-07T14:53:08","modified_gmt":"2026-09-07T07:53:08","slug":"main","status":"publish","type":"page","link":"https:\/\/sibenzyme.com\/en\/","title":{"rendered":"Main"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>Enter a name or sequence\" aria-label=\"Enter a name or sequence\"><div class=\"sbe-rebase-compact-search-buttons\"><button type=\"submit\" name=\"rebase_view\" value=\"name\" class=\"sbe-seq-search-button\">Search by name<\/button><button type=\"submit\" name=\"rebase_view\" value=\"sequence\" class=\"sbe-seq-search-button\">Search by sequence<\/button><\/div><\/form><\/div> [\/vc_column_text][vc_column_text css=&#8221;&#8221; el_class=&#8221;se-home-hero__site-search&#8221;] <\/p>\n<div class=\"se-home-search\">\n<div class=\"se-home-search__title\">\u041f\u043e\u0438\u0441\u043a \u043f\u043e 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[\/vc_column_text][\/vc_column][\/vc_row][vc_row el_id=&#8221;se-catalog&#8221; el_class=&#8221;se-home-catalog&#8221;][vc_column][vc_column_text css=&#8221;&#8221; el_class=&#8221;se-home-catalog__content&#8221;] <\/p>\n<h2 class=\"se-home-catalog__title\">\u041a\u0430\u0442\u0430\u043b\u043e\u0433<\/h2>\n<div class=\"se-home-catalog__grid\">\n<details class=\"se-home-catalog__item se-home-catalog__item--expandable\">\n<summary class=\"se-home-catalog__summary\"><span class=\"se-home-catalog__name\">\u042d\u043d\u0434\u043e\u043d\u0443\u043a\u043b\u0435\u0430\u0437\u044b \u0440\u0435\u0441\u0442\u0440\u0438\u043a\u0446\u0438\u0438<\/span><\/summary>\n<div class=\"se-home-catalog__links\"><a href=\"\/re\/\">\u0412\u0441\u0435<\/a><a href=\"\/turbo\/\">Turbo<\/a><a href=\"\/se-rebase\/\">SE REBASE<\/a><\/div>\n<\/details>\n<details class=\"se-home-catalog__item se-home-catalog__item--expandable\">\n<summary class=\"se-home-catalog__summary\"><span 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class=\"se-home-catalog__links\"><a href=\"\/product-category\/nickases\/\">\u041d\u0438\u043a\u0430\u0437\u044b<\/a><a href=\"\/product-category\/dna-methyltransferases\/\">\u0414\u041d\u041a-\u043c\u0435\u0442\u0438\u043b\u0442\u0440\u0430\u043d\u0441\u0444\u0435\u0440\u0430\u0437\u044b<\/a><\/div>\n<\/details>\n<details class=\"se-home-catalog__item se-home-catalog__item--expandable\">\n<summary class=\"se-home-catalog__summary\"><span class=\"se-home-catalog__name\">\u041f\u0440\u043e\u0447\u0435\u0435<\/span><\/summary>\n<div class=\"se-home-catalog__links\"><a href=\"\/product-category\/other\/buffers\/\">\u0411\u0443\u0444\u0435\u0440\u044b<\/a><a href=\"\/product-category\/other\/substrates\/\">\u0421\u0443\u0431\u0441\u0442\u0440\u0430\u0442\u044b<\/a><a href=\"\/product-category\/other\/primers\/\">\u041f\u0440\u0430\u0439\u043c\u0435\u0440\u044b \u0434\u043b\u044f \u0447\u0430\u0441\u0442\u043e \u0438\u0441\u043f\u043e\u043b\u044c\u0437\u0443\u0435\u043c\u044b\u0445 \u0432\u0435\u043a\u0442\u043e\u0440\u043e\u0432<\/a><a href=\"\/product-category\/other\/others\/\">\u041f\u0440\u043e\u0447\u0438\u0435 \u043f\u0440\u043e\u0434\u0443\u043a\u0442\u044b<\/a><\/div>\n<\/details>\n<\/div>\n<p> [\/vc_column_text][\/vc_column][\/vc_row][vc_row el_class=&#8221;se-home-news&#8221;][vc_column]<div class=\" bs-listing bs-listing-listing-text-3 bs-listing-single-tab\">\t\t<h3 class=\"section-heading sh-t1 sh-s1 main-term-84\">\r\n\r\n\t\t\r\n\t\t\t\t\t\t\t<a href=\"https:\/\/sibenzyme.com\/en\/category\/katalog\/\" class=\"main-link\">\r\n\t\t\t\t\t\t\t<span class=\"h-text main-term-84\">\r\n\t\t\t\t\t\t\t\t\u041d\u043e\u0432\u043e\u0441\u0442\u0438\t\t\t\t\t\t\t<\/span>\r\n\t\t\t\t<\/a>\r\n\t\t\t\r\n\t\t\r\n\t\t<\/h3>\r\n\t\t\t<div class=\"listing listing-text listing-text-3 clearfix columns-1\">\r\n\t\t<article class=\"post-4798 type-post format-standard has-post-thumbnail   listing-item listing-item-text listing-item-text-3 main-term-923\">\r\n\t<div class=\"item-inner\">\r\n\t\t<h2 class=\"title\">\t\t<a href=\"https:\/\/sibenzyme.com\/en\/2025\/english-emii-restriction-endonuclease-is-now-commercially-available\/\" class=\"post-title post-url\">\r\n\t\t\tEmiS6I Restriction Endonuclease Is Now Commercially Available\t\t<\/a>\r\n\t\t<\/h2>\t\t<div class=\"post-meta\">\r\n\r\n\t\t\t\t\t\t\t<span class=\"time\"><time class=\"post-published updated\"\r\n\t\t\t\t                         datetime=\"2025-12-26T11:42:28+07:00\">Dec 26, 2025<\/time><\/span>\r\n\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t<div class=\"post-summary\">\r\n\t\t\t\tSibEnzyme announces the commercial availability of the restriction endonuclease EmiS6I.\r\nEmiS6I is a Type IIS restriction endonuclease that recognizes the non-palindromic DNA sequence5\u2032-CTCTTC-3\u2032&hellip;\t\t\t<\/div>\r\n\t\t\t\t<\/div>\r\n\t<\/article >\r\n\t<article class=\"post-4780 type-post format-standard has-post-thumbnail   listing-item listing-item-text listing-item-text-3 main-term-85\">\r\n\t<div class=\"item-inner\">\r\n\t\t<h2 class=\"title\">\t\t<a href=\"https:\/\/sibenzyme.com\/en\/2025\/%d0%bf%d0%be%d1%81%d0%bb%d0%b5%d0%b4%d0%be%d0%b2%d0%b0%d1%82%d0%b5%d0%bb%d1%8c%d0%bd%d0%be%d1%81%d1%82%d0%b8-%d0%b4%d0%bd%d0%ba\/\" class=\"post-title post-url\">\r\n\t\t\t(\u0420\u0443\u0441\u0441\u043a\u0438\u0439) \u041f\u043e\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u0442\u0435\u043b\u044c\u043d\u043e\u0441\u0442\u0438 \u0414\u041d\u041a\t\t<\/a>\r\n\t\t<\/h2>\t\t<div class=\"post-meta\">\r\n\r\n\t\t\t\t\t\t\t<span class=\"time\"><time class=\"post-published updated\"\r\n\t\t\t\t                         datetime=\"2025-12-08T09:58:22+07:00\">Dec 8, 2025<\/time><\/span>\r\n\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t<div class=\"post-summary\">\r\n\t\t\t\tSorry, this entry is only available in \u0420\u0443\u0441\u0441\u043a\u0438\u0439.\t\t\t<\/div>\r\n\t\t\t\t<\/div>\r\n\t<\/article >\r\n\t<article class=\"post-4778 type-post format-standard has-post-thumbnail   listing-item listing-item-text listing-item-text-3 main-term-85\">\r\n\t<div class=\"item-inner\">\r\n\t\t<h2 class=\"title\">\t\t<a href=\"https:\/\/sibenzyme.com\/en\/2025\/%d0%b8%d0%b7%d0%bc%d0%b5%d0%bd%d0%b5%d0%bd%d0%b8%d1%8f-%d0%bd%d0%b0%d0%bb%d0%be%d0%b3%d0%be%d0%b2%d0%be%d0%b3%d0%be-%d0%b7%d0%b0%d0%ba%d0%be%d0%bd%d0%be%d0%b4%d0%b0%d1%82%d0%b5%d0%bb%d1%8c%d1%81%d1%82\/\" class=\"post-title post-url\">\r\n\t\t\t(\u0420\u0443\u0441\u0441\u043a\u0438\u0439) \u0418\u0437\u043c\u0435\u043d\u0435\u043d\u0438\u044f \u043d\u0430\u043b\u043e\u0433\u043e\u0432\u043e\u0433\u043e \u0437\u0430\u043a\u043e\u043d\u043e\u0434\u0430\u0442\u0435\u043b\u044c\u0441\u0442\u0432\u0430\t\t<\/a>\r\n\t\t<\/h2>\t\t<div class=\"post-meta\">\r\n\r\n\t\t\t\t\t\t\t<span class=\"time\"><time class=\"post-published updated\"\r\n\t\t\t\t                         datetime=\"2025-12-01T09:54:16+07:00\">Dec 1, 2025<\/time><\/span>\r\n\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t<div class=\"post-summary\">\r\n\t\t\t\tSorry, this entry is only available in \u0420\u0443\u0441\u0441\u043a\u0438\u0439.\t\t\t<\/div>\r\n\t\t\t\t<\/div>\r\n\t<\/article >\r\n\t<article class=\"post-4729 type-post format-standard has-post-thumbnail   listing-item listing-item-text listing-item-text-3 main-term-923\">\r\n\t<div class=\"item-inner\">\r\n\t\t<h2 class=\"title\">\t\t<a href=\"https:\/\/sibenzyme.com\/en\/2025\/novyj-ferment-bmo-i\/\" class=\"post-title post-url\">\r\n\t\t\tNew restriction endonuclease Bmo I\t\t<\/a>\r\n\t\t<\/h2>\t\t<div class=\"post-meta\">\r\n\r\n\t\t\t\t\t\t\t<span class=\"time\"><time class=\"post-published updated\"\r\n\t\t\t\t                         datetime=\"2025-11-21T11:33:48+07:00\">Nov 21, 2025<\/time><\/span>\r\n\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t<div class=\"post-summary\">\r\n\t\t\t\tWe are pleased to announce the release of a new restriction endonuclease \u2014 the large-cleaving enzyme BmoI.BmoI recognizes the palindromic heptanucleotide sequence 5\u2019-GG\u2193GWCCC-3\u2019 (W = A or T) and&hellip;\t\t\t<\/div>\r\n\t\t\t\t<\/div>\r\n\t<\/article >\r\n\t<article class=\"post-4630 type-post format-standard has-post-thumbnail   listing-item listing-item-text listing-item-text-3 main-term-85\">\r\n\t<div class=\"item-inner\">\r\n\t\t<h2 class=\"title\">\t\t<a href=\"https:\/\/sibenzyme.com\/en\/2025\/novye-czeny\/\" class=\"post-title post-url\">\r\n\t\t\t(\u0420\u0443\u0441\u0441\u043a\u0438\u0439) \u041d\u043e\u0432\u044b\u0435 \u0446\u0435\u043d\u044b\t\t<\/a>\r\n\t\t<\/h2>\t\t<div class=\"post-meta\">\r\n\r\n\t\t\t\t\t\t\t<span class=\"time\"><time class=\"post-published updated\"\r\n\t\t\t\t                         datetime=\"2025-09-15T15:29:57+07:00\">Sep 15, 2025<\/time><\/span>\r\n\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t<div class=\"post-summary\">\r\n\t\t\t\tSorry, this entry is only available in \u0420\u0443\u0441\u0441\u043a\u0438\u0439.\t\t\t<\/div>\r\n\t\t\t\t<\/div>\r\n\t<\/article >\r\n\t<\/div>\r\n\t<\/div>[vc_column_text css=&#8221;&#8221; el_class=&#8221;se-home-news__all&#8221;]<a href=\"\/category\/novosti\/\">\u0412\u0441\u0435 \u043d\u043e\u0432\u043e\u0441\u0442\u0438 \u2192<\/a>[\/vc_column_text][\/vc_column][\/vc_row][vc_row el_class=&#8221;se-home-publications&#8221;][vc_column][vc_column_text css=&#8221;&#8221; el_class=&#8221;se-home-publications__content&#8221;] <\/p>\n<h2 class=\"se-home-publications__title\">\u041f\u043e\u0441\u043b\u0435\u0434\u043d\u0438\u0435 \u043f\u0443\u0431\u043b\u0438\u043a\u0430\u0446\u0438\u0438<\/h2>\n<div class=\"sepub-list sepub-embedded sepub-latest \"><ol class=\"sepub-items\"><li class=\"sepub-item\"><article><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><a class=\"sepub-latest-title\" href=\"#sepub-details-1-0\" data-sepub-details=\"sepub-details-1-0\" aria-haspopup=\"dialog\">The Pse31I restriction endonuclease from the bacterial strain Peribacillus species 31, an isozomere of Eco31I, recognizes the non-palindromic DNA sequence 5\u2019-GGTCTC-3\u2019 (1\/5)<\/a><span class=\"sepub-authors\">V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, N.A. Vorontsova, M.A. Abdurashitov, D.A. Gonchar, S.Kh. Degtyarev<\/span><\/article><dialog class=\"sepub-details\" id=\"sepub-details-1-0\" aria-labelledby=\"sepub-details-1-0-title\"><button type=\"button\" class=\"sepub-details-close\" aria-label=\"Close\">\u00d7<\/button><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><h2 id=\"sepub-details-1-0-title\">The Pse31I restriction endonuclease from the bacterial strain Peribacillus species 31, an isozomere of Eco31I, recognizes the non-palindromic DNA sequence 5\u2019-GGTCTC-3\u2019 (1\/5)<\/h2><p class=\"sepub-authors\">V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, N.A. Vorontsova, M.A. Abdurashitov, D.A. Gonchar, S.Kh. Degtyarev<\/p><p class=\"sepub-bibliography\">DNA-Recognizing Enzymes, Vol. 2025(1), DOI: 10.26213\/3034-4301.2025.4.1.001 (2025). (In Russian)<\/p><details class=\"sepub-abstract\"><summary>Abstract<\/summary><p>We have identified a bacterial strain, Peribacillus species 31, which produces a novel restriction endonuclease named Pse31I. The enzyme recognizes the six-nucleotide non-palindromic DNA sequence 5\u2019-GGTCTC-3\u2019 and cleaves it downstream of the recognition site at positions 1\/5: 5\u2019-GGTCTC(N)1^\/3\u2019-CCAGAG(N)5^-5\u2019. Thus, Pse31I is a true isozomere of the Eco31I restriction enzyme. The producing strain was identified based on morphological and biochemical characteristics, as well as on analysis of the primary structure of a fragment of the 16S rRNA gene. Pse31I exhibits maximum activity at 37\u00b0C and is inactivated by incubation at 55 and 65\u00b0C. The enzyme does not cleave sites containing methylated cytosine in the upper strand (5\u2019-GGTCT(5mC)-3\u2019) and\/or in the lower strand (3\u2019-(5mC)CAGAG) .<br \/>\n<br \/>\nThe optimal conditions for enzyme activity are SE Buffer 5 (Y) (33 mM Tris-acetate (pH 7.9), 10 mM Mg-acetate, 66 mM K-acetate, 1 mM DTT) and a temperature of 37\u00b0C.<br \/>\n<br \/>\nA PecI restriction endonuclease preparation with a concentration of 10,000 units\/mL was obtained through purification in four chromatographic stages. The optimal conditions for the enzyme reaction are SE Buffer 5 (Y) (33 mM Tris-acetate (pH 7.9), 10 mM Mg-acetate, 66 mM K-acetate, 1 mM DTT) and a temperature of 37\u00b0C.<\/p><\/details><p class=\"sepub-details-links\"><a href=\"https:\/\/dnape.online\/ru\/2025\/03\/pse31-i\/\" target=\"_blank\" rel=\"noopener noreferrer\">Full text (EN)<\/a> <a href=\"https:\/\/doi.org\/10.26213\/3034-4301.2025.4.1.001\" target=\"_blank\" rel=\"noopener noreferrer\">DOI: 10.26213\/3034-4301.2025.4.1.001<\/a> <\/p><\/dialog><\/li><li class=\"sepub-item\"><article><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><a class=\"sepub-latest-title\" href=\"#sepub-details-1-1\" data-sepub-details=\"sepub-details-1-1\" aria-haspopup=\"dialog\">The BmoI restriction endonuclease from the bacterial strain Brevundimonas mongoliensis 53 recognizes the DNA sequence 5\u2019-GG^GWCCC-3\u2019.<\/a><span class=\"sepub-authors\">D.A. Gonchar, M.A. Abdurashitov, V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, V.E. Ryzhova, S.Kh. Degtyarev<\/span><\/article><dialog class=\"sepub-details\" id=\"sepub-details-1-1\" aria-labelledby=\"sepub-details-1-1-title\"><button type=\"button\" class=\"sepub-details-close\" aria-label=\"Close\">\u00d7<\/button><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><h2 id=\"sepub-details-1-1-title\">The BmoI restriction endonuclease from the bacterial strain Brevundimonas mongoliensis 53 recognizes the DNA sequence 5\u2019-GG^GWCCC-3\u2019.<\/h2><p class=\"sepub-authors\">D.A. Gonchar, M.A. Abdurashitov, V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, V.E. Ryzhova, S.Kh. Degtyarev<\/p><p class=\"sepub-bibliography\">DNA-Recognizing Enzymes, vol. 2025(2), DOI: 10.26213\/3034-4301.2025.5.2.002 (2025). (In Russian)<\/p><details class=\"sepub-abstract\"><summary>Abstract<\/summary><p>We have identified a bacterial strain, Brevundimonas mongoliensis 53, which produces a novel large-cleavage restriction endonuclease named BmoI. The BmoI enzyme recognizes the seven-nucleotide palindromic DNA sequence 5\u2019-GG\u2193GWCCC-3\u2019 (W = A or T) and cleaves it as indicated by the arrow, forming three-nucleotide<br \/>\n5\u2019-overhanging \u201csticky\u201d ends. BmoI is an isozomere of the previously described restriction endonuclease SanDI. The producer strain was identified based on morphological and biochemical characteristics, as well as on analysis of the primary structure of a fragment of the 16S rRNA gene. A preparation of the BmoI restriction endonuclease with a concentration of 2,000 units\/mL was obtained by purification through three chromatographic stages. The optimal conditions for enzyme activity are SE buffer W (10 mM Tris-HCl (pH 8.5), 10 mM MgCl\u2082, 100 mM NaCl, 1 mM DTT) and a reaction temperature of 37\u00b0C.<\/p><\/details><p class=\"sepub-details-links\"><a href=\"https:\/\/dnape.online\/ru\/2025\/11\/bmoi\/\" target=\"_blank\" rel=\"noopener noreferrer\">Full text (EN)<\/a> <a href=\"https:\/\/doi.org\/10.26213\/3034-4301.2025.5.2.002\" target=\"_blank\" rel=\"noopener noreferrer\">DOI: 10.26213\/3034-4301.2025.5.2.002<\/a> <\/p><\/dialog><\/li><li class=\"sepub-item\"><article><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><a class=\"sepub-latest-title\" href=\"#sepub-details-1-2\" data-sepub-details=\"sepub-details-1-2\" aria-haspopup=\"dialog\">The EmiS6I restriction endonuclease, the Ksp632I isoisomer, recognizes the non-palindromic DNA sequence 5\u2019-CTCTTC(1\/4)-3\u2019.<\/a><span class=\"sepub-authors\">M.A. Abdurashitov, D.A. Gonchar, V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, A.A. Nikonova, S.Kh. Degtyarev<\/span><\/article><dialog class=\"sepub-details\" id=\"sepub-details-1-2\" aria-labelledby=\"sepub-details-1-2-title\"><button type=\"button\" class=\"sepub-details-close\" aria-label=\"Close\">\u00d7<\/button><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><h2 id=\"sepub-details-1-2-title\">The EmiS6I restriction endonuclease, the Ksp632I isoisomer, recognizes the non-palindromic DNA sequence 5\u2019-CTCTTC(1\/4)-3\u2019.<\/h2><p class=\"sepub-authors\">M.A. Abdurashitov, D.A. Gonchar, V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, A.A. Nikonova, S.Kh. Degtyarev<\/p><p class=\"sepub-bibliography\">DNA-Recognizing Enzymes, Vol. 2025(4), DOI: 10.26213\/3034-4301.2025.7.4.002 (2025). (In Russian)<\/p><details class=\"sepub-abstract\"><summary>Abstract<\/summary><p>We have identified a bacterial strain, Exiguobacterium mexicanum 6, which produces a novel restriction endonuclease named EmiS6I. The EmiS6I enzyme recognizes the six-nucleotide non-palindromic DNA sequence 5\u2019-CTCTTC-3\u2019 and cleaves it downstream of the recognition site at positions 1\/4: 5\u2019-CTCTTC(N)1\u2193-3\u2019\/3\u2019-GAGAAG(N)4\u2193-5\u2019, forming 5\u2019-protruding trinucleotide ends. Thus, EmiS6I is an isozysomer of the restriction enzymes Ksp632I and Bst6I. The producer strain was identified based on morphological and biochemical characteristics, as well as on analysis of the primary structure of a fragment of the 16S rRNA gene. A preparation of the EmiS6I restriction endonuclease with a concentration of 5,000 units\/mL was obtained by purification through three chromatographic steps. The optimal conditions for enzyme activity are SE Buffer Y (33 mM Tris-acetate (pH 7.9), 10 mM Mg-acetate, 66 mM K-acetate, 1 mM DTT) and a temperature of 37\u00b0C.<\/p><\/details><p class=\"sepub-details-links\"><a href=\"https:\/\/dnape.online\/ru\/2025\/12\/emi-i\/\" target=\"_blank\" rel=\"noopener noreferrer\">Full text (EN)<\/a> <a href=\"https:\/\/doi.org\/10.26213\/3034-4301.2025.7.4.002\" target=\"_blank\" rel=\"noopener noreferrer\">DOI: 10.26213\/3034-4301.2025.7.4.002<\/a> <\/p><\/dialog><\/li><li class=\"sepub-item\"><article><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><a class=\"sepub-latest-title\" href=\"#sepub-details-1-3\" data-sepub-details=\"sepub-details-1-3\" aria-haspopup=\"dialog\">Determination of the Methylation Status of RCGY Sites in the Human Genome: A Comparison of NGS Results from GlaI Hydrolysates and Data from GlaI-PCR Analysis<\/a><span class=\"sepub-authors\">M.A. Abdurashitov, A.G. Akishev, S.Kh. Degtyarev<\/span><\/article><dialog class=\"sepub-details\" id=\"sepub-details-1-3\" aria-labelledby=\"sepub-details-1-3-title\"><button type=\"button\" class=\"sepub-details-close\" aria-label=\"Close\">\u00d7<\/button><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><h2 id=\"sepub-details-1-3-title\">Determination of the Methylation Status of RCGY Sites in the Human Genome: A Comparison of NGS Results from GlaI Hydrolysates and Data from GlaI-PCR Analysis<\/h2><p class=\"sepub-authors\">M.A. Abdurashitov, A.G. Akishev, S.Kh. Degtyarev<\/p><p class=\"sepub-bibliography\">Epigenetic DNA Diagnostics, Vol. 2025(1), DOI: 10.26213\/3034-4298.2025.6.1.001 (2025). (In Russian)<\/p><details class=\"sepub-abstract\"><summary>Abstract<\/summary><p>The 5-methylcytosine-dependent DNA endonuclease GlaI recognizes and cleaves 5\u2032-R(5mC)GY-3\u2032 \/ 3\u2032-YG(5mC)R-5\u2032 sites in DNA, which are formed de novo in the human genome by the action of the DNA methyltransferase DNMT3. Due to its specificity, the GlaI enzyme is effectively used to determine the methylation status of RCGY sites in the human genome. Genomic mapping of R(5mC)GY sites is performed using next-generation sequencing (NGS) of DNA hydrolysates obtained by GlaI digestion, whereas analysis of individual DNA fragments can be performed using GlaI-PCR.<br \/>\n<br \/>\nIn this study, we compared the results of NGS analysis of GlaI-digested DNA hydrolysates with those of GlaI-PCR analysis of the regulatory regions of 11 tumor-suppressor genes in the DNA of the human cell lines L68 (normal lung fibroblasts), Raji (Burkitt\u2019s lymphoma), and U937 (histiocytic lymphoma). It was shown that in L68 cell DNA, most of the regulatory regions studied are unmethylated, whereas in the DNA of the malignant cell lines Raji and U937, methylation of these regions is observed. With the exception of one region, for all other fragments studied, the methylation status determined by GlaI-PCR analysis fully correlates with the number of R(5mC)GY sites identified by NGS. The only discrepancy between the data from the two methods was identified in the regulatory region of the RASSF1A gene in L68 cell DNA, which is explained by the presence of allele-specific methylation, confirmed by an additional real-time GlaI-PCR analysis. Given the presence of allelic methylation in the regulatory region of the RASSF1A gene, we can conclude that there is complete agreement between the NGS data for GlaI hydrolysates and the GlaI-PCR analysis of the regulatory regions of the studied tumor suppressor genes in the human cell lines L68, Raji, and U937.<br \/>\n<br \/>\nThus, NGS analysis of GlaI-hydrolyzed DNA and GlaI-PCR analysis are complementary methods that enable the reliable identification of abnormally methylated genomic regions in normal and tumor cells, making them promising tools for epigenetic research and DNA diagnostics.<\/p><\/details><p class=\"sepub-details-links\"><a href=\"https:\/\/www.epigendx.online\/ru\/2025\/ngv-vs-pcr\/\" target=\"_blank\" rel=\"noopener noreferrer\">Full text (EN)<\/a> <a href=\"https:\/\/doi.org\/10.26213\/3034-4298.2025.6.1.001\" target=\"_blank\" rel=\"noopener noreferrer\">DOI: 10.26213\/3034-4298.2025.6.1.001<\/a> <\/p><\/dialog><\/li><li class=\"sepub-item\"><article><time class=\"sepub-year\" datetime=\"2024\">2024<\/time><a class=\"sepub-latest-title\" href=\"#sepub-details-1-4\" data-sepub-details=\"sepub-details-1-4\" aria-haspopup=\"dialog\">The PecI restriction endonuclease from the bacterial strain Paracoccus species 12 recognizes the DNA sequence 5\u2019-TTA^TAA-3\u2019 and is an isozomere of PsiI.<\/a><span class=\"sepub-authors\">D.A. Gonchar, V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, M.A. Abdurashitov, O.A. Belichenko, S.Kh. Degtyarev<\/span><\/article><dialog class=\"sepub-details\" id=\"sepub-details-1-4\" aria-labelledby=\"sepub-details-1-4-title\"><button type=\"button\" class=\"sepub-details-close\" aria-label=\"Close\">\u00d7<\/button><time class=\"sepub-year\" datetime=\"2024\">2024<\/time><h2 id=\"sepub-details-1-4-title\">The PecI restriction endonuclease from the bacterial strain Paracoccus species 12 recognizes the DNA sequence 5\u2019-TTA^TAA-3\u2019 and is an isozomere of PsiI.<\/h2><p class=\"sepub-authors\">D.A. Gonchar, V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, M.A. Abdurashitov, O.A. Belichenko, S.Kh. Degtyarev<\/p><p class=\"sepub-bibliography\">DNA-recognizing enzymes, vol. 2024(1), DOI: 10.26213\/SE.2024.47.21.001 (2024). (In Russian)<\/p><details class=\"sepub-abstract\"><summary>Abstract<\/summary><p>We have identified a bacterial strain, Paracoccus species 12, that produces a novel restriction endonuclease named PecI. This enzyme recognizes the six-nucleotide palindromic DNA sequence 5\u2019-TTA^TAA-3\u2019 and cleaves it to form \u201cblunt\u201d ends, as indicated by the arrow. Thus, PecI is a true isozym of the PsiI restriction endonuclease.<br \/>\r\n<br \/>\r\nA PecI restriction endonuclease preparation with a concentration of 10,000 units\/mL was obtained through a four-step chromatographic purification process. The optimal reaction conditions for the enzyme are SE Buffer 5 (Y) (33 mM Tris-acetate (pH 7.9), 10 mM Mg-acetate, 66 mM K-acetate, 1 mM DTT) and a temperature of 37\u00b0C.<\/p><\/details><p class=\"sepub-details-links\"><a href=\"https:\/\/dnape.online\/ru\/2024\/10\/peci\/\" target=\"_blank\" rel=\"noopener noreferrer\">Full text (EN)<\/a> <a href=\"https:\/\/doi.org\/10.26213\/SE.2024.47.21.001\" target=\"_blank\" rel=\"noopener noreferrer\">DOI: 10.26213\/SE.2024.47.21.001<\/a> <\/p><\/dialog><\/li><li class=\"sepub-item sepub-all-publications\"><article><a class=\"sepub-latest-title\" href=\"https:\/\/sibenzyme.com\/publications\/\">All publications<\/a><\/article><\/li><\/ol><\/div> [\/vc_column_text][\/vc_column][\/vc_row][vc_row el_id=&#8221;se-about&#8221; el_class=&#8221;se-home-about&#8221;][vc_column][vc_column_text css=&#8221;&#8221; el_class=&#8221;se-home-about__content&#8221;] <\/p>\n<h2 class=\"se-home-about__title\">\u041e \u043a\u043e\u043c\u043f\u0430\u043d\u0438\u0438<\/h2>\n<div class=\"se-home-about__facts\">\n<div class=\"se-home-about__fact\">\n<div class=\"se-home-about__value\">30+ \u043b\u0435\u0442<\/div>\n<div class=\"se-home-about__label\">\u0440\u0430\u0437\u0440\u0430\u0431\u043e\u0442\u043a\u0438 \u0438 \u043f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0441\u0442\u0432\u0430 \u0444\u0435\u0440\u043c\u0435\u043d\u0442\u043e\u0432<\/div><\/div>\n<div class=\"se-home-about__fact\">\n<div class=\"se-home-about__value\">350+<\/div>\n<div class=\"se-home-about__label\">\u0441\u043e\u0431\u0441\u0442\u0432\u0435\u043d\u043d\u044b\u0445 \u043f\u0440\u043e\u0434\u0443\u043a\u0442\u043e\u0432<\/div><\/div>\n<div class=\"se-home-about__fact\">\n<div class=\"se-home-about__value\">200+<\/div>\n<div class=\"se-home-about__label\">\u044d\u043d\u0434\u043e\u043d\u0443\u043a\u043b\u0435\u0430\u0437 \u0440\u0435\u0441\u0442\u0440\u0438\u043a\u0446\u0438\u0438<\/div><\/div>\n<div class=\"se-home-about__fact\">\n<div class=\"se-home-about__value\">78<\/div>\n<div class=\"se-home-about__label\">\u0440\u0435\u0441\u0442\u0440\u0438\u043a\u0442\u0430\u0437 Turbo<\/div><\/div><\/div>\n<div class=\"se-home-about__footer\"><a class=\"se-home-about__link\" href=\"\/about\/\">\u041e \u043a\u043e\u043c\u043f\u0430\u043d\u0438\u0438 \u2192<\/a><\/div>\n<p> [\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<p>[vc_row el_class=&#8221;se-home-hero&#8221;][vc_column width=&#8221;7\/12&#8243; 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Phage DNA<\/a><\/div>\n<\/details>\n<details class=\"se-home-catalog__item se-home-catalog__item--expandable\">\n<summary class=\"se-home-catalog__summary\"><span class=\"se-home-catalog__name\">dNTPs<\/span><\/summary>\n<div class=\"se-home-catalog__links\"><a href=\"\/product-category\/dntps\/enz-dntps\/\">Enzymatic Synthesis<\/a><a href=\"\/product-category\/dntps\/chem-dntps\/\">Chemical Synthesis<\/a><\/div>\n<\/details>\n<p><a class=\"se-home-catalog__item se-home-catalog__item--direct\" href=\"\/product-category\/kits\/\"><span class=\"se-home-catalog__name\">Kits<\/span><\/a><\/p>\n<details class=\"se-home-catalog__item se-home-catalog__item--expandable\">\n<summary class=\"se-home-catalog__summary\"><span class=\"se-home-catalog__name\">Other Enzymes<\/span><\/summary>\n<div class=\"se-home-catalog__links\"><a href=\"\/product-category\/nickases\/\">Nickases<\/a><a href=\"\/product-category\/dna-methyltransferases\/\">DNA Methyltransferases<\/a><\/div>\n<\/details>\n<details class=\"se-home-catalog__item se-home-catalog__item--expandable\">\n<summary class=\"se-home-catalog__summary\"><span class=\"se-home-catalog__name\">Other Products<\/span><\/summary>\n<div class=\"se-home-catalog__links\"><a href=\"\/product-category\/other\/buffers\/\">Buffers<\/a><a href=\"\/product-category\/other\/substrates\/\">Substrates<\/a><a href=\"\/product-category\/other\/primers\/\">Primers for Commonly Used Vectors<\/a><a href=\"\/product-category\/other\/others\/\">Other Products<\/a><\/div>\n<\/details>\n<\/div>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row el_class=&#8221;se-home-news&#8221;][vc_column]<div class=\" bs-listing bs-listing-listing-text-3 bs-listing-single-tab\">\t\t<h3 class=\"section-heading sh-t1 sh-s1 main-term-923\">\r\n\r\n\t\t\r\n\t\t\t\t\t\t\t<a href=\"https:\/\/sibenzyme.com\/en\/category\/english\/\" class=\"main-link\">\r\n\t\t\t\t\t\t\t<span class=\"h-text main-term-923\">\r\n\t\t\t\t\t\t\t\tNews\t\t\t\t\t\t\t<\/span>\r\n\t\t\t\t<\/a>\r\n\t\t\t\r\n\t\t\r\n\t\t<\/h3>\r\n\t\t\t<div class=\"listing listing-text listing-text-3 clearfix columns-1\">\r\n\t\t<article class=\"post-4798 type-post format-standard has-post-thumbnail   listing-item listing-item-text listing-item-text-3 main-term-923\">\r\n\t<div class=\"item-inner\">\r\n\t\t<h2 class=\"title\">\t\t<a href=\"https:\/\/sibenzyme.com\/en\/2025\/english-emii-restriction-endonuclease-is-now-commercially-available\/\" class=\"post-title post-url\">\r\n\t\t\tEmiS6I Restriction Endonuclease Is Now Commercially Available\t\t<\/a>\r\n\t\t<\/h2>\t\t<div class=\"post-meta\">\r\n\r\n\t\t\t\t\t\t\t<span class=\"time\"><time class=\"post-published updated\"\r\n\t\t\t\t                         datetime=\"2025-12-26T11:42:28+07:00\">Dec 26, 2025<\/time><\/span>\r\n\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t<div class=\"post-summary\">\r\n\t\t\t\tSibEnzyme announces the commercial availability of the restriction endonuclease EmiS6I.\r\nEmiS6I is a Type IIS restriction endonuclease that recognizes the non-palindromic DNA sequence5\u2032-CTCTTC-3\u2032&hellip;\t\t\t<\/div>\r\n\t\t\t\t<\/div>\r\n\t<\/article >\r\n\t<article class=\"post-4729 type-post format-standard has-post-thumbnail   listing-item listing-item-text listing-item-text-3 main-term-923\">\r\n\t<div class=\"item-inner\">\r\n\t\t<h2 class=\"title\">\t\t<a href=\"https:\/\/sibenzyme.com\/en\/2025\/novyj-ferment-bmo-i\/\" class=\"post-title post-url\">\r\n\t\t\tNew restriction endonuclease Bmo I\t\t<\/a>\r\n\t\t<\/h2>\t\t<div class=\"post-meta\">\r\n\r\n\t\t\t\t\t\t\t<span class=\"time\"><time class=\"post-published updated\"\r\n\t\t\t\t                         datetime=\"2025-11-21T11:33:48+07:00\">Nov 21, 2025<\/time><\/span>\r\n\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t<div class=\"post-summary\">\r\n\t\t\t\tWe are pleased to announce the release of a new restriction endonuclease \u2014 the large-cleaving enzyme BmoI.BmoI recognizes the palindromic heptanucleotide sequence 5\u2019-GG\u2193GWCCC-3\u2019 (W = A or T) and&hellip;\t\t\t<\/div>\r\n\t\t\t\t<\/div>\r\n\t<\/article >\r\n\t<article class=\"post-4272 type-post format-standard has-post-thumbnail   listing-item listing-item-text listing-item-text-3 main-term-923\">\r\n\t<div class=\"item-inner\">\r\n\t\t<h2 class=\"title\">\t\t<a href=\"https:\/\/sibenzyme.com\/en\/2025\/novyj-ferment-pse-31-i-istinnyj-izoshizomer-restriktazy-eco-31-i\/\" class=\"post-title post-url\">\r\n\t\t\tThe new enzyme Pse31I is a true isoschizomer of the restriction endonuclease Eco31I (Bso31I)\t\t<\/a>\r\n\t\t<\/h2>\t\t<div class=\"post-meta\">\r\n\r\n\t\t\t\t\t\t\t<span class=\"time\"><time class=\"post-published updated\"\r\n\t\t\t\t                         datetime=\"2025-02-28T17:29:39+07:00\">Feb 28, 2025<\/time><\/span>\r\n\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t<div class=\"post-summary\">\r\n\t\t\t\tWe have discovered a bacterial strain, Peribacillus species 31, which produces a new restriction endonuclease named Pse31I. This enzyme recognizes the six-nucleotide non-palindromic sequence&hellip;\t\t\t<\/div>\r\n\t\t\t\t<\/div>\r\n\t<\/article >\r\n\t<article class=\"post-4187 type-post format-standard has-post-thumbnail   listing-item listing-item-text listing-item-text-3 main-term-923\">\r\n\t<div class=\"item-inner\">\r\n\t\t<h2 class=\"title\">\t\t<a href=\"https:\/\/sibenzyme.com\/en\/2024\/novyj-ferment-pec-i-istinnyj-izoshizomer-restriktazy-psi-i\/\" class=\"post-title post-url\">\r\n\t\t\tNew Enzyme PecI \u2014 a True Isoschizomer of the Restriction Endonuclease PsiI\t\t<\/a>\r\n\t\t<\/h2>\t\t<div class=\"post-meta\">\r\n\r\n\t\t\t\t\t\t\t<span class=\"time\"><time class=\"post-published updated\"\r\n\t\t\t\t                         datetime=\"2024-10-28T11:30:47+07:00\">Oct 28, 2024<\/time><\/span>\r\n\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t<div class=\"post-summary\">\r\n\t\t\t\tWe have identified a bacterial strain, Paracoccus species 12, which is a producer of a novel restriction endonuclease designated PecI. The enzyme recognizes the palindromic hexanucleotide DNA&hellip;\t\t\t<\/div>\r\n\t\t\t\t<\/div>\r\n\t<\/article >\r\n\t<article class=\"post-3487 type-post format-standard has-post-thumbnail   listing-item listing-item-text listing-item-text-3 main-term-923\">\r\n\t<div class=\"item-inner\">\r\n\t\t<h2 class=\"title\">\t\t<a href=\"https:\/\/sibenzyme.com\/en\/2023\/rasshirenie-assortimenta-fermentov-dlya-bystrogo-gidroliza\/\" class=\"post-title post-url\">\r\n\t\t\tNew Turbo enzymes for fast DNA digestion\t\t<\/a>\r\n\t\t<\/h2>\t\t<div class=\"post-meta\">\r\n\r\n\t\t\t\t\t\t\t<span class=\"time\"><time class=\"post-published updated\"\r\n\t\t\t\t                         datetime=\"2023-03-02T10:42:22+07:00\">Mar 2, 2023<\/time><\/span>\r\n\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t<div class=\"post-summary\">\r\n\t\t\t\tNow we have 72 Turbo enzymes\t\t\t<\/div>\r\n\t\t\t\t<\/div>\r\n\t<\/article >\r\n\t<\/div>\r\n\t<\/div>[vc_column_text css=&#8221;&#8221; el_class=&#8221;se-home-news__all&#8221;]<a href=\"\/category\/novosti\/\">All news \u2192<\/a>[\/vc_column_text][\/vc_column][\/vc_row][vc_row el_class=&#8221;se-home-publications&#8221;][vc_column][vc_column_text css=&#8221;&#8221; el_class=&#8221;se-home-publications__content&#8221;]<\/p>\n<h2 class=\"se-home-publications__title\">Latest Publications<\/h2>\n<div class=\"sepub-list sepub-embedded sepub-latest \"><ol class=\"sepub-items\"><li class=\"sepub-item\"><article><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><a class=\"sepub-latest-title\" href=\"#sepub-details-2-0\" data-sepub-details=\"sepub-details-2-0\" aria-haspopup=\"dialog\">The Pse31I restriction endonuclease from the bacterial strain Peribacillus species 31, an isozomere of Eco31I, recognizes the non-palindromic DNA sequence 5\u2019-GGTCTC-3\u2019 (1\/5)<\/a><span class=\"sepub-authors\">V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, N.A. Vorontsova, M.A. Abdurashitov, D.A. Gonchar, S.Kh. Degtyarev<\/span><\/article><dialog class=\"sepub-details\" id=\"sepub-details-2-0\" aria-labelledby=\"sepub-details-2-0-title\"><button type=\"button\" class=\"sepub-details-close\" aria-label=\"Close\">\u00d7<\/button><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><h2 id=\"sepub-details-2-0-title\">The Pse31I restriction endonuclease from the bacterial strain Peribacillus species 31, an isozomere of Eco31I, recognizes the non-palindromic DNA sequence 5\u2019-GGTCTC-3\u2019 (1\/5)<\/h2><p class=\"sepub-authors\">V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, N.A. Vorontsova, M.A. Abdurashitov, D.A. Gonchar, S.Kh. Degtyarev<\/p><p class=\"sepub-bibliography\">DNA-Recognizing Enzymes, Vol. 2025(1), DOI: 10.26213\/3034-4301.2025.4.1.001 (2025). (In Russian)<\/p><details class=\"sepub-abstract\"><summary>Abstract<\/summary><p>We have identified a bacterial strain, Peribacillus species 31, which produces a novel restriction endonuclease named Pse31I. The enzyme recognizes the six-nucleotide non-palindromic DNA sequence 5\u2019-GGTCTC-3\u2019 and cleaves it downstream of the recognition site at positions 1\/5: 5\u2019-GGTCTC(N)1^\/3\u2019-CCAGAG(N)5^-5\u2019. Thus, Pse31I is a true isozomere of the Eco31I restriction enzyme. The producing strain was identified based on morphological and biochemical characteristics, as well as on analysis of the primary structure of a fragment of the 16S rRNA gene. Pse31I exhibits maximum activity at 37\u00b0C and is inactivated by incubation at 55 and 65\u00b0C. The enzyme does not cleave sites containing methylated cytosine in the upper strand (5\u2019-GGTCT(5mC)-3\u2019) and\/or in the lower strand (3\u2019-(5mC)CAGAG) .<br \/>\n<br \/>\nThe optimal conditions for enzyme activity are SE Buffer 5 (Y) (33 mM Tris-acetate (pH 7.9), 10 mM Mg-acetate, 66 mM K-acetate, 1 mM DTT) and a temperature of 37\u00b0C.<br \/>\n<br \/>\nA PecI restriction endonuclease preparation with a concentration of 10,000 units\/mL was obtained through purification in four chromatographic stages. The optimal conditions for the enzyme reaction are SE Buffer 5 (Y) (33 mM Tris-acetate (pH 7.9), 10 mM Mg-acetate, 66 mM K-acetate, 1 mM DTT) and a temperature of 37\u00b0C.<\/p><\/details><p class=\"sepub-details-links\"><a href=\"https:\/\/dnape.online\/ru\/2025\/03\/pse31-i\/\" target=\"_blank\" rel=\"noopener noreferrer\">Full text (EN)<\/a> <a href=\"https:\/\/doi.org\/10.26213\/3034-4301.2025.4.1.001\" target=\"_blank\" rel=\"noopener noreferrer\">DOI: 10.26213\/3034-4301.2025.4.1.001<\/a> <\/p><\/dialog><\/li><li class=\"sepub-item\"><article><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><a class=\"sepub-latest-title\" href=\"#sepub-details-2-1\" data-sepub-details=\"sepub-details-2-1\" aria-haspopup=\"dialog\">The BmoI restriction endonuclease from the bacterial strain Brevundimonas mongoliensis 53 recognizes the DNA sequence 5\u2019-GG^GWCCC-3\u2019.<\/a><span class=\"sepub-authors\">D.A. Gonchar, M.A. Abdurashitov, V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, V.E. Ryzhova, S.Kh. Degtyarev<\/span><\/article><dialog class=\"sepub-details\" id=\"sepub-details-2-1\" aria-labelledby=\"sepub-details-2-1-title\"><button type=\"button\" class=\"sepub-details-close\" aria-label=\"Close\">\u00d7<\/button><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><h2 id=\"sepub-details-2-1-title\">The BmoI restriction endonuclease from the bacterial strain Brevundimonas mongoliensis 53 recognizes the DNA sequence 5\u2019-GG^GWCCC-3\u2019.<\/h2><p class=\"sepub-authors\">D.A. Gonchar, M.A. Abdurashitov, V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, V.E. Ryzhova, S.Kh. Degtyarev<\/p><p class=\"sepub-bibliography\">DNA-Recognizing Enzymes, vol. 2025(2), DOI: 10.26213\/3034-4301.2025.5.2.002 (2025). (In Russian)<\/p><details class=\"sepub-abstract\"><summary>Abstract<\/summary><p>We have identified a bacterial strain, Brevundimonas mongoliensis 53, which produces a novel large-cleavage restriction endonuclease named BmoI. The BmoI enzyme recognizes the seven-nucleotide palindromic DNA sequence 5\u2019-GG\u2193GWCCC-3\u2019 (W = A or T) and cleaves it as indicated by the arrow, forming three-nucleotide<br \/>\n5\u2019-overhanging \u201csticky\u201d ends. BmoI is an isozomere of the previously described restriction endonuclease SanDI. The producer strain was identified based on morphological and biochemical characteristics, as well as on analysis of the primary structure of a fragment of the 16S rRNA gene. A preparation of the BmoI restriction endonuclease with a concentration of 2,000 units\/mL was obtained by purification through three chromatographic stages. The optimal conditions for enzyme activity are SE buffer W (10 mM Tris-HCl (pH 8.5), 10 mM MgCl\u2082, 100 mM NaCl, 1 mM DTT) and a reaction temperature of 37\u00b0C.<\/p><\/details><p class=\"sepub-details-links\"><a href=\"https:\/\/dnape.online\/ru\/2025\/11\/bmoi\/\" target=\"_blank\" rel=\"noopener noreferrer\">Full text (EN)<\/a> <a href=\"https:\/\/doi.org\/10.26213\/3034-4301.2025.5.2.002\" target=\"_blank\" rel=\"noopener noreferrer\">DOI: 10.26213\/3034-4301.2025.5.2.002<\/a> <\/p><\/dialog><\/li><li class=\"sepub-item\"><article><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><a class=\"sepub-latest-title\" href=\"#sepub-details-2-2\" data-sepub-details=\"sepub-details-2-2\" aria-haspopup=\"dialog\">The EmiS6I restriction endonuclease, the Ksp632I isoisomer, recognizes the non-palindromic DNA sequence 5\u2019-CTCTTC(1\/4)-3\u2019.<\/a><span class=\"sepub-authors\">M.A. Abdurashitov, D.A. Gonchar, V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, A.A. Nikonova, S.Kh. Degtyarev<\/span><\/article><dialog class=\"sepub-details\" id=\"sepub-details-2-2\" aria-labelledby=\"sepub-details-2-2-title\"><button type=\"button\" class=\"sepub-details-close\" aria-label=\"Close\">\u00d7<\/button><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><h2 id=\"sepub-details-2-2-title\">The EmiS6I restriction endonuclease, the Ksp632I isoisomer, recognizes the non-palindromic DNA sequence 5\u2019-CTCTTC(1\/4)-3\u2019.<\/h2><p class=\"sepub-authors\">M.A. Abdurashitov, D.A. Gonchar, V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, A.A. Nikonova, S.Kh. Degtyarev<\/p><p class=\"sepub-bibliography\">DNA-Recognizing Enzymes, Vol. 2025(4), DOI: 10.26213\/3034-4301.2025.7.4.002 (2025). (In Russian)<\/p><details class=\"sepub-abstract\"><summary>Abstract<\/summary><p>We have identified a bacterial strain, Exiguobacterium mexicanum 6, which produces a novel restriction endonuclease named EmiS6I. The EmiS6I enzyme recognizes the six-nucleotide non-palindromic DNA sequence 5\u2019-CTCTTC-3\u2019 and cleaves it downstream of the recognition site at positions 1\/4: 5\u2019-CTCTTC(N)1\u2193-3\u2019\/3\u2019-GAGAAG(N)4\u2193-5\u2019, forming 5\u2019-protruding trinucleotide ends. Thus, EmiS6I is an isozysomer of the restriction enzymes Ksp632I and Bst6I. The producer strain was identified based on morphological and biochemical characteristics, as well as on analysis of the primary structure of a fragment of the 16S rRNA gene. A preparation of the EmiS6I restriction endonuclease with a concentration of 5,000 units\/mL was obtained by purification through three chromatographic steps. The optimal conditions for enzyme activity are SE Buffer Y (33 mM Tris-acetate (pH 7.9), 10 mM Mg-acetate, 66 mM K-acetate, 1 mM DTT) and a temperature of 37\u00b0C.<\/p><\/details><p class=\"sepub-details-links\"><a href=\"https:\/\/dnape.online\/ru\/2025\/12\/emi-i\/\" target=\"_blank\" rel=\"noopener noreferrer\">Full text (EN)<\/a> <a href=\"https:\/\/doi.org\/10.26213\/3034-4301.2025.7.4.002\" target=\"_blank\" rel=\"noopener noreferrer\">DOI: 10.26213\/3034-4301.2025.7.4.002<\/a> <\/p><\/dialog><\/li><li class=\"sepub-item\"><article><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><a class=\"sepub-latest-title\" href=\"#sepub-details-2-3\" data-sepub-details=\"sepub-details-2-3\" aria-haspopup=\"dialog\">Determination of the Methylation Status of RCGY Sites in the Human Genome: A Comparison of NGS Results from GlaI Hydrolysates and Data from GlaI-PCR Analysis<\/a><span class=\"sepub-authors\">M.A. Abdurashitov, A.G. Akishev, S.Kh. Degtyarev<\/span><\/article><dialog class=\"sepub-details\" id=\"sepub-details-2-3\" aria-labelledby=\"sepub-details-2-3-title\"><button type=\"button\" class=\"sepub-details-close\" aria-label=\"Close\">\u00d7<\/button><time class=\"sepub-year\" datetime=\"2025\">2025<\/time><h2 id=\"sepub-details-2-3-title\">Determination of the Methylation Status of RCGY Sites in the Human Genome: A Comparison of NGS Results from GlaI Hydrolysates and Data from GlaI-PCR Analysis<\/h2><p class=\"sepub-authors\">M.A. Abdurashitov, A.G. Akishev, S.Kh. Degtyarev<\/p><p class=\"sepub-bibliography\">Epigenetic DNA Diagnostics, Vol. 2025(1), DOI: 10.26213\/3034-4298.2025.6.1.001 (2025). (In Russian)<\/p><details class=\"sepub-abstract\"><summary>Abstract<\/summary><p>The 5-methylcytosine-dependent DNA endonuclease GlaI recognizes and cleaves 5\u2032-R(5mC)GY-3\u2032 \/ 3\u2032-YG(5mC)R-5\u2032 sites in DNA, which are formed de novo in the human genome by the action of the DNA methyltransferase DNMT3. Due to its specificity, the GlaI enzyme is effectively used to determine the methylation status of RCGY sites in the human genome. Genomic mapping of R(5mC)GY sites is performed using next-generation sequencing (NGS) of DNA hydrolysates obtained by GlaI digestion, whereas analysis of individual DNA fragments can be performed using GlaI-PCR.<br \/>\n<br \/>\nIn this study, we compared the results of NGS analysis of GlaI-digested DNA hydrolysates with those of GlaI-PCR analysis of the regulatory regions of 11 tumor-suppressor genes in the DNA of the human cell lines L68 (normal lung fibroblasts), Raji (Burkitt\u2019s lymphoma), and U937 (histiocytic lymphoma). It was shown that in L68 cell DNA, most of the regulatory regions studied are unmethylated, whereas in the DNA of the malignant cell lines Raji and U937, methylation of these regions is observed. With the exception of one region, for all other fragments studied, the methylation status determined by GlaI-PCR analysis fully correlates with the number of R(5mC)GY sites identified by NGS. The only discrepancy between the data from the two methods was identified in the regulatory region of the RASSF1A gene in L68 cell DNA, which is explained by the presence of allele-specific methylation, confirmed by an additional real-time GlaI-PCR analysis. Given the presence of allelic methylation in the regulatory region of the RASSF1A gene, we can conclude that there is complete agreement between the NGS data for GlaI hydrolysates and the GlaI-PCR analysis of the regulatory regions of the studied tumor suppressor genes in the human cell lines L68, Raji, and U937.<br \/>\n<br \/>\nThus, NGS analysis of GlaI-hydrolyzed DNA and GlaI-PCR analysis are complementary methods that enable the reliable identification of abnormally methylated genomic regions in normal and tumor cells, making them promising tools for epigenetic research and DNA diagnostics.<\/p><\/details><p class=\"sepub-details-links\"><a href=\"https:\/\/www.epigendx.online\/ru\/2025\/ngv-vs-pcr\/\" target=\"_blank\" rel=\"noopener noreferrer\">Full text (EN)<\/a> <a href=\"https:\/\/doi.org\/10.26213\/3034-4298.2025.6.1.001\" target=\"_blank\" rel=\"noopener noreferrer\">DOI: 10.26213\/3034-4298.2025.6.1.001<\/a> <\/p><\/dialog><\/li><li class=\"sepub-item\"><article><time class=\"sepub-year\" datetime=\"2024\">2024<\/time><a class=\"sepub-latest-title\" href=\"#sepub-details-2-4\" data-sepub-details=\"sepub-details-2-4\" aria-haspopup=\"dialog\">The PecI restriction endonuclease from the bacterial strain Paracoccus species 12 recognizes the DNA sequence 5\u2019-TTA^TAA-3\u2019 and is an isozomere of PsiI.<\/a><span class=\"sepub-authors\">D.A. Gonchar, V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, M.A. Abdurashitov, O.A. Belichenko, S.Kh. Degtyarev<\/span><\/article><dialog class=\"sepub-details\" id=\"sepub-details-2-4\" aria-labelledby=\"sepub-details-2-4-title\"><button type=\"button\" class=\"sepub-details-close\" aria-label=\"Close\">\u00d7<\/button><time class=\"sepub-year\" datetime=\"2024\">2024<\/time><h2 id=\"sepub-details-2-4-title\">The PecI restriction endonuclease from the bacterial strain Paracoccus species 12 recognizes the DNA sequence 5\u2019-TTA^TAA-3\u2019 and is an isozomere of PsiI.<\/h2><p class=\"sepub-authors\">D.A. Gonchar, V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, M.A. Abdurashitov, O.A. Belichenko, S.Kh. Degtyarev<\/p><p class=\"sepub-bibliography\">DNA-recognizing enzymes, vol. 2024(1), DOI: 10.26213\/SE.2024.47.21.001 (2024). (In Russian)<\/p><details class=\"sepub-abstract\"><summary>Abstract<\/summary><p>We have identified a bacterial strain, Paracoccus species 12, that produces a novel restriction endonuclease named PecI. This enzyme recognizes the six-nucleotide palindromic DNA sequence 5\u2019-TTA^TAA-3\u2019 and cleaves it to form \u201cblunt\u201d ends, as indicated by the arrow. Thus, PecI is a true isozym of the PsiI restriction endonuclease.<br \/>\r\n<br \/>\r\nA PecI restriction endonuclease preparation with a concentration of 10,000 units\/mL was obtained through a four-step chromatographic purification process. The optimal reaction conditions for the enzyme are SE Buffer 5 (Y) (33 mM Tris-acetate (pH 7.9), 10 mM Mg-acetate, 66 mM K-acetate, 1 mM DTT) and a temperature of 37\u00b0C.<\/p><\/details><p class=\"sepub-details-links\"><a href=\"https:\/\/dnape.online\/ru\/2024\/10\/peci\/\" target=\"_blank\" rel=\"noopener noreferrer\">Full text (EN)<\/a> <a href=\"https:\/\/doi.org\/10.26213\/SE.2024.47.21.001\" target=\"_blank\" rel=\"noopener noreferrer\">DOI: 10.26213\/SE.2024.47.21.001<\/a> <\/p><\/dialog><\/li><li class=\"sepub-item sepub-all-publications\"><article><a class=\"sepub-latest-title\" href=\"https:\/\/sibenzyme.com\/publications\/\">All publications<\/a><\/article><\/li><\/ol><\/div><br \/>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row el_id=&#8221;se-about&#8221; el_class=&#8221;se-home-about&#8221;][vc_column][vc_column_text css=&#8221;&#8221; el_class=&#8221;se-home-about__content&#8221;]<\/p>\n<h2 class=\"se-home-about__title\">About Us<\/h2>\n<div class=\"se-home-about__facts\">\n<div class=\"se-home-about__fact\">\n<div class=\"se-home-about__value\">30+ years<\/div>\n<div class=\"se-home-about__label\">of enzyme development and manufacturing<\/div>\n<\/div>\n<div class=\"se-home-about__fact\">\n<div class=\"se-home-about__value\">350+<\/div>\n<div class=\"se-home-about__label\">proprietary products<\/div>\n<\/div>\n<div class=\"se-home-about__fact\">\n<div class=\"se-home-about__value\">200+<\/div>\n<div class=\"se-home-about__label\">restriction endonucleases<\/div>\n<\/div>\n<div class=\"se-home-about__fact\">\n<div class=\"se-home-about__value\">78<\/div>\n<div class=\"se-home-about__label\">Turbo restriction enzymes<\/div>\n<\/div>\n<\/div>\n<div class=\"se-home-about__footer\"><a class=\"se-home-about__link\" href=\"\/about\/\">About Us \u2192<\/a><\/div>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<p><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row el_class=&#8221;se-home-hero&#8221;][vc_column width=&#8221;7\/12&#8243; el_class=&#8221;se-home-hero__intro&#8221;][vc_column_text css=&#8221;&#8221; el_class=&#8221;se-home-hero__content&#8221;] SibEnzyme \u2014 enzymes for molecular biology One of the world&#8217;s leading developers and manufacturers of restriction endonucleases. 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