{"id":3026,"date":"2022-05-17T10:51:14","date_gmt":"2022-05-17T03:51:14","guid":{"rendered":"http:\/\/sibenzyme.com\/?page_id=3026"},"modified":"2025-10-09T11:54:15","modified_gmt":"2025-10-09T04:54:15","slug":"aktivity","status":"publish","type":"page","link":"https:\/\/sibenzyme.com\/en\/aktivity\/","title":{"rendered":"SE-buffers activity"},"content":{"rendered":"

<\/p>\n

SE-buffers activity and inactivation temperature (20 minutes)<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Enzyme<\/span><\/b><\/td>\nRecognition sequence<\/span><\/b><\/td>\nOptimal buffer<\/span><\/b><\/td>\nBSA<\/span><\/b><\/td>\n% Activity<\/span><\/b><\/td>\nOptimal temperature, \u00b0\u0421<\/span><\/b><\/td>\nInactivation, \u00b0\u0421<\/span><\/b><\/td>\n<\/tr>\n
B<\/b><\/span><\/td>\nG<\/span><\/b><\/td>\nO<\/span><\/b><\/td>\nW<\/span><\/b><\/td>\nY<\/span><\/b><\/td>\nRose<\/span><\/b><\/td>\n<\/tr>\n
Aat II<\/td>\n
GACGT\u2191<\/b>C<\/span>
\nC\u2193<\/b>TGCAG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Abs I<\/td>\n
CC\u2191<\/b>TCGAGG<\/span>
\nGGAGCT\u2193<\/b>CC<\/span><\/center><\/td>\n
*<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Acc16 I<\/td>\n
TGC\u2191<\/b>GCA<\/span>
\nACG\u2193<\/b>CGT<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n70<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Acc36 I<\/td>\n
ACCTGC(N)4<\/sub>\u2191<\/b><\/span>
\nTGGACG(N)8<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Acc65 I<\/td>\n
G\u2191<\/b>GTACC<\/span>
\nCCATG\u2193<\/b>G<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AccB1 I<\/td>\n
G\u2191<\/b>GYRCC<\/span>
\nCCRYG\u2193<\/b>G<\/span><\/center><\/td>\n
K<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n30<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AccB7 I<\/td>\n
CCANNNN\u2191<\/b>NTGG<\/span>
\nGGTN\u2193<\/b>NNNNACC<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AccBS I<\/td>\n
GAG\u2191<\/b>CGG<\/span>
\nCTC\u2193<\/b>GCC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Acl I<\/td>\n
AA\u2191<\/b>CGTT<\/span>
\nTTGC\u2193<\/b>AA<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n80<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AclW I<\/td>\n
GGATC(N)4<\/sub>\u2191<\/b><\/span>
\nCCTAG(N)5<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n30<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Aco I<\/td>\n
Y\u2191<\/b>GGCCR<\/span>
\nRCCGG\u2193<\/b>Y<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Acs I<\/td>\n
R\u2191<\/b>AATTY<\/span>
\nYTTAA\u2193<\/b>R<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n
Acu I<\/td>\n
CTGAAG(N)16<\/sub>\u2191<\/b><\/span>
\nGACTTC(N)14<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y+ SAM<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Afe I<\/td>\n
AGC\u2191<\/b>GCT<\/span>
\nTCG\u2193<\/b>CGA<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Ags I<\/td>\n
TTS\u2191<\/b>AA<\/span>
\nAA\u2193<\/b>STT<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Ahl I<\/td>\n
A\u2191<\/b>CTAGT<\/span>
\nTGATC\u2193<\/b>A<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\nN<\/td>\n<\/tr>\n
Ajn I<\/td>\n
\u2191<\/b>CCWGG<\/span>
\nGGWCC\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n
Alu I<\/td>\n
AG\u2191<\/b>CT<\/span>
\nTC\u2193<\/b>GA<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AluB I<\/td>\n
AG\u2191<\/b>CT<\/span>
\nTC\u2193<\/b>GA<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n60<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Ama87 I<\/td>\n
C\u2191<\/b>YCGRG<\/span>
\nGRGCY\u2193<\/b>C<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n0 – 10<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Apa I<\/td>\n
GGGCC\u2191<\/b>C<\/span>
\nC\u2193<\/b>CCGGG<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Ars I<\/td>\n
\u2191<\/b>(N)8<\/sub>GACNNNNNNTTYG(N)11<\/sub>\u2191<\/b><\/span>
\n\u2193<\/b>(N)13<\/sub>CTGNNNNNNAARC(N)6<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n20<\/span><\/td>\n30<\/td>\n65<\/td>\n<\/tr>\n
AsiG I<\/td>\n
A\u2191<\/b>CCGGT<\/span>
\nTGGCC\u2193<\/b>A<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n75<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AspA2 I<\/td>\n
C\u2191<\/b>CTAGG<\/span>
\nGGATC\u2193<\/b>C<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
AspLE I<\/td>\n
GCG\u2191<\/b>C<\/span>
\nC\u2193<\/b>GCG<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\nN<\/td>\n<\/tr>\n
AspS9 I<\/td>\n
G\u2191<\/b>GNCC<\/span>
\nCCNG\u2193<\/b>G<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n75<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AsuC2 I<\/td>\n
CC\u2191<\/b>SGG<\/span>
\nGGS\u2193<\/b>CC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n40<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AsuHP I<\/td>\n
GGTGA(N)8<\/sub>\u2191<\/b><\/span>
\nCCACT(N)7<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AsuNH I<\/td>\n
G\u2191<\/b>CTAGC<\/span>
\nCGATC\u2193<\/b>G<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BamH I<\/td>\n
G\u2191<\/b>GATCC<\/span>
\nCCTAG\u2193<\/b>G<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bar I<\/td>\n
\u2191<\/b>(N)7<\/sub>GAAGNNNNNNTAC(N)12<\/sub>\u2191<\/b><\/span>
\n\u2193<\/b>(N)12<\/sub>CTTCNNNNNNATG(N)7<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
*<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n40<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bbv12 I<\/td>\n
GWGCW\u2191<\/b>C<\/span>
\nC\u2193<\/b>WCGWG<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n60<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Bgl I<\/td>\n
GCCNNNN\u2191<\/b>NGGC<\/span>
\nCGGN\u2193<\/b>NNNNCCG<\/span><\/center><\/td>\n
2W<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bgl II<\/td>\n
A\u2191<\/b>GATCT<\/span>
\nTCTAG\u2193<\/b>A<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Bme18 I<\/td>\n
G\u2191<\/b>GWCC<\/span>
\nCCWG\u2193<\/b>G<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n80<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bmt I<\/td>\n
GCTAG\u2191<\/b>C<\/span>
\nC\u2193<\/b>GATCG<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bmu I<\/td>\n
ACTGGG(N)5<\/sub>\u2191<\/b><\/span>
\nTGACCC(N)4<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BpmI<\/td>\n
CTGGAG(N)16<\/sub>\u2191<\/b><\/span>
\nGACCTC(N)14<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bpu10I<\/td>\n
CC\u2191<\/b>TNAGC<\/span>
\nGGANT\u2193<\/b>CG<\/span><\/center><\/td>\n
K<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Bpu14 I<\/td>\n
TT\u2191<\/b>CGAA<\/span>
\nAAGC\u2193<\/b>TT<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bsa29 I<\/td>\n
AT\u2191<\/b>CGAT<\/span>
\nTAGC\u2193<\/b>TA<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bsc4 I<\/td>\n
CCNNNNN\u2191<\/b>NNGG<\/span>
\nGGNN\u2193<\/b>NNNNNCC<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n90<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
Bse1 I<\/td>\n
ACTGGN\u2191<\/b><\/span>
\nTGAC\u2193<\/b>CN<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
Bse118 I<\/td>\n
R\u2191<\/b>CCGGY<\/span>
\nYGGCC\u2193<\/b>R<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
Bse21 I<\/td>\n
CC\u2191<\/b>TNAGG<\/span>
\nGGANT\u2193<\/b>CC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n40<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Bse3D I<\/td>\n
GCAATGNN\u2191<\/b><\/span>
\nCGTTAC\u2193<\/b>NN<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
Bse8 I<\/td>\n
GATNN\u2191<\/b>NNATC<\/span>
\nCTANN\u2193<\/b>NNTAG<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
BseP I<\/td>\n
G\u2191<\/b>CGCGC<\/span>
\nCGCGC\u2193<\/b>G<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50<\/td>\n65<\/td>\n<\/tr>\n
BseX3 I<\/td>\n
C\u2191<\/b>GGCCG<\/span>
\nGCCGG\u2193<\/b>C<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25<\/span><\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n
BslF I<\/td>\n
GGGAC(N)10<\/sub>\u2191<\/b><\/span>
\nCCCTG(N)14<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Bso31 I<\/td>\n
GGTCTC(N)1<\/sub>\u2191<\/b><\/span>
\nCCAGAG(N)5<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
O<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n40<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
Bsp13 I<\/td>\n
T\u2191<\/b>CCGGA<\/span>
\nAGGCC\u2193<\/b>T<\/span><\/center><\/td>\n
2K<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n5<\/span><\/td>\n50<\/td>\n65<\/td>\n<\/tr>\n
Bsp1720 I<\/td>\n
GC\u2191<\/b>TNAGC<\/span>
\nCGANT\u2193<\/b>CG<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Bsp19 I<\/td>\n
C\u2191<\/b>CATGG<\/span>
\nGGTAC\u2193<\/b>C<\/span><\/center><\/td>\n
2W<\/td>\n+<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n5<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BspAC I<\/td>\n
C\u2191<\/b>CGC<\/span>
\nGGC\u2193<\/b>G<\/span><\/center><\/td>\n
O<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BspFN I<\/td>\n
CG\u2191<\/b>CG<\/span>
\nGC\u2193<\/b>GC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BssEC I<\/td>\n
C\u2191<\/b>CNNGG<\/span>
\nGGNNC\u2193<\/b>C<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
BssNA I<\/td>\n
GTA\u2191<\/b>TAC<\/span>
\nCAT\u2193<\/b>ATG<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\nN<\/td>\n<\/tr>\n
BssT1 I<\/td>\n
C\u2191<\/b>CWWGG<\/span>
\nGGWWC\u2193<\/b>C<\/span><\/center><\/td>\n
2K<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
Bst2B I<\/td>\n
C\u2191<\/b>TCGTG<\/span>
\nGAGCA\u2193<\/b>C<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
Bst2U I<\/td>\n
CC\u2191<\/b>WGG<\/span>
\nGGW\u2193<\/b>CC<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n50<\/span><\/td>\n60<\/td>\nN<\/td>\n<\/tr>\n
Bst4C I<\/td>\n
ACN\u2191<\/b>GT<\/span>
\nTG\u2193<\/b>NCA<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n65<\/td>\nN<\/td>\n<\/tr>\n
Bst6 I<\/td>\n
CTCTTC(N)1<\/sub>\u2191<\/b><\/span>
\nGAGAAG(N)4<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
BstAC I<\/td>\n
GR\u2191<\/b>CGYC<\/span>
\nCYGC\u2193<\/b>RG<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
BstAF I<\/td>\n
C\u2191<\/b>TTAAG<\/span>
\nGAATT\u2193<\/b>C<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
BstAP I<\/td>\n
GCANNNN\u2191<\/b>NTGC<\/span>
\nCGTN\u2193<\/b>NNNNACG<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
BstAU I<\/td>\n
T\u2191<\/b>GTACA<\/span>
\nACATG\u2193<\/b>T<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
BstBA I<\/td>\n
YAC\u2191<\/b>GTR<\/span>
\nRTG\u2193<\/b>CAY<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
BstC8I<\/td>\n
GCN\u2191<\/b>NGC<\/span>
\nCGN\u2193<\/b>NCG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
BstDE I<\/td>\n
C\u2191<\/b>TNAG<\/span>
\nGANT\u2193<\/b>C<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
BstDS I<\/td>\n
C\u2191<\/b>CRYGG<\/span>
\nGGYRC\u2193<\/b>C<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
BstEN I<\/td>\n
CCTNN\u2191<\/b>NNNAGG<\/span>
\nGGANNN\u2193<\/b>NNTCC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
BstF5 I<\/td>\n
GGATGNN\u2191<\/b><\/span>
\nCCTAC\u2193<\/b>NN<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
BstFN I<\/td>\n
CG\u2191<\/b>CG<\/span>
\nGC\u2193<\/b>GC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
BstH2 I<\/td>\n
RGCGC\u2191<\/b>Y<\/span>
\nY\u2193<\/b>CGCGR<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
BstHH I<\/td>\n
GCG\u2191<\/b>C<\/span>
\nC\u2193<\/b>GCG<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n50<\/td>\nN<\/td>\n<\/tr>\n
BstKTI<\/td>\n
GAT\u2191<\/b>C<\/span>
\nC\u2193<\/b>TAG<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BstMA I<\/td>\n
GTCTC(N)1<\/sub>\u2191<\/b><\/span>
\nCAGAG(N)5<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n
BstMB I<\/td>\n
\u2191<\/b>GATC<\/span>
\nCTAG\u2193<\/b><\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
BstMC I<\/td>\n
CGRY\u2191<\/b>CG<\/span>
\nGC\u2193<\/b>YRGC<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n
BstMW I<\/td>\n
GCNNNNN\u2191<\/b>NNGC<\/span>
\nCGNN\u2193<\/b>NNNNNCG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n40<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
BstNS I<\/td>\n
RCATG\u2191<\/b>Y<\/span>
\nY\u2193<\/b>GTACR<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BstPA I<\/td>\n
GACNN\u2191<\/b>NNGTC<\/span>
\nCTGNN\u2193<\/b>NNCAG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\nN<\/td>\n<\/tr>\n
BstSCI<\/td>\n
\u2191<\/b>CCNGG<\/span>
\nGGNCC\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n60<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
BstSF I<\/td>\n
C\u2191<\/b>TRYAG<\/span>
\nGAYRT\u2193<\/b>C<\/span><\/center><\/td>\n
O<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\nN<\/td>\n<\/tr>\n
BstSL I<\/td>\n
GKGCM\u2191<\/b>C<\/span>
\nC\u2193<\/b>MCGKG<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n
BstSN I<\/td>\n
TAC\u2191<\/b>GTA<\/span>
\nATG\u2193<\/b>CAT<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
BstV1I<\/td>\n
GCAGC(N)8<\/sub>\u2191<\/b><\/span>
\nCGTCG(N)12<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
BstV2 I<\/td>\n
GAAGAC(N)2<\/sub>\u2191<\/b><\/span>
\nCTTCTG(N)6<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n70<\/span><\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n
BstX I<\/td>\n
CCANNNNN\u2191<\/b>NTGG<\/span>
\nGGTN\u2193<\/b>NNNNNACC<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BstX2 I Temporarily unavailable<\/span><\/td>\n
R\u2191<\/b>GATCY<\/span>
\nYCTAG\u2193<\/b>R<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
Bsu I<\/td>\n
GTATCC(N)6<\/sub>\u2191<\/b><\/span>
\nCATAGG(N)5<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n10<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BsuR I<\/td>\n
GG\u2191<\/b>CC<\/span>
\nCC\u2193<\/b>GG<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Btr I<\/td>\n
CAC\u2191<\/b>GTC<\/span>
\nGTG\u2193<\/b>CAG<\/span><\/center><\/td>\n
O<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
Cci I<\/td>\n
T\u2191<\/b>CATGA<\/span>
\nAGTAC\u2193<\/b>T<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
CciN I<\/td>\n
GC\u2191<\/b>GGCCGC<\/span>
\nCGCCGG\u2193<\/b>CG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Dra I<\/td>\n
TTT\u2191<\/b>AAA<\/span>
\nAAA\u2193<\/b>TTT<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
DraIII<\/td>\n
CACNNN\u2191<\/b>GTG<\/span>
\nGTG\u2193<\/b>NNNCAC<\/span><\/center><\/td>\n
2K<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Dri I<\/td>\n
GACNNN\u2191<\/b>NNGTC<\/span>
\nCTGNN\u2193<\/b>NNNCAG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n40<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
DseD I<\/td>\n
GACNNNN\u2191<\/b>NNGTC<\/span>
\nCTGNN\u2193<\/b>NNNNCAG<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n30<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
EcoICR I<\/td>\n
GAG\u2191<\/b>CTC<\/span>
\nCTC\u2193<\/b>GAG<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n75 – 100<\/span><\/td>\n5<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
EcoR I<\/td>\n
G\u2191<\/b>AATTC<\/span>
\nCTTAA\u2193<\/b>G<\/span><\/center><\/td>\n
*<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
EcoR V<\/td>\n
GAT\u2191<\/b>ATC<\/span>
\nCTA\u2193<\/b>TAG<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n0 – 10<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Ege I<\/td>\n
GGC\u2191<\/b>GCC<\/span>
\nCCG\u2193<\/b>CGG<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Erh I<\/td>\n
C\u2191<\/b>CWWGG<\/span>
\nGGWWC\u2193<\/b>C<\/span><\/center><\/td>\n
2W<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
FaeI<\/td>\n
CATG\u2191<\/b><\/span>
\n\u2193<\/b>GTAC<\/span><\/center><\/td>\n
*<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
FaiI<\/td>\n
YA\u2191<\/b>TR<\/span>
\nRT\u2193<\/b>AY<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n
FalI<\/td>\n
\u2191<\/b>(N)8<\/sub>AAGNNNNNCTT(N)13<\/sub>\u2191<\/b><\/span>
\n\u2193<\/b>(N)13<\/sub>TTCNNNNNGAA(N)8<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
W+ SAM<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n70<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
FatI<\/td>\n
\u2191<\/b>CATG<\/span>
\nGTAC\u2193<\/b><\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n
Fau I<\/td>\n
CCCGC(N)4<\/sub>\u2191<\/b><\/span>
\nGGGCG(N)6<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n50 – 75<\/span><\/td>\n50<\/span><\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n
FauND I<\/td>\n
CA\u2191<\/b>TATG<\/span>
\nGTAT\u2193<\/b>AC<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Fbl I<\/td>\n
GT\u2191<\/b>MKAC<\/span>
\nCAKM\u2193<\/b>TG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n0 – 10<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
Fok I<\/td>\n
GGATG(N)9<\/sub>\u2191<\/b><\/span>
\nCCTAC(N)13<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
FriO I<\/td>\n
GRGCY\u2191<\/b>C<\/span>
\nC\u2193<\/b>YCGRG<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Fsp4H I<\/td>\n
GC\u2191<\/b>NGC<\/span>
\nCGN\u2193<\/b>CG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Gsa I<\/td>\n
CCCAG\u2191<\/b>C<\/span>
\nG\u2193<\/b>GGTCG<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n70<\/td>\nN<\/td>\n<\/tr>\n
Hae III<\/td>\n
GG\u2191<\/b>CC<\/span>
\nCC\u2193<\/b>GG<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Hga I<\/td>\n
GACGC(N)5<\/sub>\u2191<\/b><\/span>
\nCTGCG(N)10<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Hind II<\/td>\n
GTY\u2191<\/b>RAC<\/span>
\nCAR\u2193<\/b>YTG<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Hind III<\/td>\n
A\u2191<\/b>AGCTT<\/span>
\nTTCGA\u2193<\/b>A<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Hinf I<\/td>\n
G\u2191<\/b>ANTC<\/span>
\nCTNA\u2193<\/b>G<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Hpa I<\/td>\n
GTT\u2191<\/b>AAC<\/span>
\nCAA\u2193<\/b>TTG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Hpa II<\/td>\n
C\u2191<\/b>CGG<\/span>
\nGGC\u2193<\/b>C<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
HpySE526 I<\/td>\n
A\u2191<\/b>CGT<\/span>
\nTGC\u2193<\/b>A<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
HspA I<\/td>\n
G\u2191<\/b>CGC<\/span>
\nCGC\u2193<\/b>G<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Kpn I<\/td>\n
GGTAC\u2191<\/b>C<\/span>
\nC\u2193<\/b>CATGG<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Ksp22 I<\/td>\n
T\u2191<\/b>GATCA<\/span>
\nACTAG\u2193<\/b>T<\/span><\/center><\/td>\n
2K<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Kzo9 I<\/td>\n
\u2191<\/b>GATC<\/span>
\nCTAG\u2193<\/b><\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Lmn I<\/td>\n
GCTCCN\u2191<\/b><\/span>
\nCGAG\u2193<\/b>GN<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n80<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Mab I<\/td>\n
A\u2191<\/b>CCWGGT<\/span>
\nTGGWCC\u2193<\/b>A<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Mbo II<\/td>\n
GAAGA(N)8<\/sub>\u2191<\/b><\/span>
\nCTTCT(N)7<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Mfe I<\/td>\n
C\u2191<\/b>AATTG<\/span>
\nGTTAA\u2193<\/b>C<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\nN<\/td>\n<\/tr>\n
Mhl I<\/td>\n
GDGCH\u2191<\/b>C<\/span>
\nC\u2193<\/b>HCGDG<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Mlu I<\/td>\n
A\u2191<\/b>CGCGT<\/span>
\nTGCGC\u2193<\/b>A<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Mly113 I<\/td>\n
GG\u2191<\/b>CGCC<\/span>
\nCCGC\u2193<\/b>GG<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Mnl I<\/td>\n
CCTC(N)7<\/sub>\u2191<\/b><\/span>
\nGGAG(N)6<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Mox20 I<\/td>\n
TGG\u2191<\/b>CCA<\/span>
\nACC\u2193<\/b>GGT<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n75<\/span><\/td>\n37<\/td>\nN<\/td>\n<\/tr>\n
MroN I<\/td>\n
G\u2191<\/b>CCGGC<\/span>
\nCGGCC\u2193<\/b>G<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n5<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
MroX I<\/td>\n
GAANN\u2191<\/b>NNTTC<\/span>
\nCTTNN\u2193<\/b>NNAAG<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Msp I<\/td>\n
C\u2191<\/b>CGG<\/span>
\nGGC\u2193<\/b>C<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
MspA1 I<\/td>\n
CMG\u2191<\/b>CKG<\/span>
\nGKC\u2193<\/b>GMC<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
MspR9 I<\/td>\n
CC\u2191<\/b>NGG<\/span>
\nGGN\u2193<\/b>CC<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Nru I<\/td>\n
TCG\u2191<\/b>CGA<\/span>
\nAGC\u2193<\/b>GCT<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
PalA I<\/td>\n
GG\u2191<\/b>CGCGCC<\/span>
\nCCGCGC\u2193<\/b>GG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n40<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Pec I<\/td>\n
TTA\u2191TAA<\/center>
AAT\u2193ATT<\/center><\/td>\n
Y<\/td>\n–<\/td>\n50-75<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Pce I<\/td>\n
AGG\u2191<\/b>CCT<\/span>
\nTCC\u2193<\/b>GGA<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n
Pci I<\/td>\n
A\u2191<\/b>CATGT<\/span>
\nTGTAC\u2193<\/b>A<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
PciS I<\/td>\n
GCTCTTCN\u2191<\/b><\/span>
\nCGAGAAG(N)4<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n75 – 100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Pct I<\/td>\n
GAATGCN\u2191<\/b><\/span>
\nCTTAC\u2193<\/b>GN<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Ple19 I<\/td>\n
CGAT\u2191<\/b>CG<\/span>
\nGC\u2193<\/b>TAGC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Pps I<\/td>\n
GAGTC(N)4<\/sub>\u2191<\/b><\/span>
\nCTCAG(N)5<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Pse31 I<\/td>\n
GGTCTC(N)1<\/sub>\u2191<\/b><\/span>
\nCCAGAG(N)5<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n25-50<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Psi I<\/td>\n
TTA\u2191<\/b>TAA<\/span>
\nAAT\u2193<\/b>ATT<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n40<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Psp124B I<\/td>\n
GAGCT\u2191<\/b>C<\/span>
\nC\u2193<\/b>TCGAG<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n75 – 100<\/span><\/td>\n30<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Psp6 I<\/td>\n
\u2191<\/b>CCWGG<\/span>
\nGGWCC\u2193<\/b><\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n10<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
PspC I<\/td>\n
CAC\u2191<\/b>GTG<\/span>
\nGTG\u2193<\/b>CAC<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n50 – 75<\/span><\/td>\n5<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
PspE I<\/td>\n
G\u2191<\/b>GTNACC<\/span>
\nCCANTG\u2193<\/b>G<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
PspL I<\/td>\n
C\u2191<\/b>GTACG<\/span>
\nGCATG\u2193<\/b>C<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
PspN4 I<\/td>\n
GGN\u2191<\/b>NCC<\/span>
\nCCN\u2193<\/b>NGG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
PspOM I<\/td>\n
G\u2191<\/b>GGCCC<\/span>
\nCCCGG\u2193<\/b>G<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
PspPP I<\/td>\n
RG\u2191<\/b>GWCCY<\/span>
\nYCCWG\u2193<\/b>GR<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
PspX I<\/td>\n
VC\u2191<\/b>TCGAGB<\/span>
\nBGAGCT\u2193<\/b>CV<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Psr I<\/td>\n
\u2191<\/b>(N)7<\/sub>GAACNNNNNNTAC(N)12<\/sub>\u2191<\/b><\/span>
\n\u2193<\/b>(N)12<\/sub>CTTGNNNNNNATG(N)7<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n30<\/span><\/td>\n30<\/td>\n65<\/td>\n<\/tr>\n
Pst I<\/td>\n
CTGCA\u2191<\/b>G<\/span>
\nG\u2193<\/b>ACGTC<\/span><\/center><\/td>\n
O<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
PstN I<\/td>\n
CAGNNN\u2191<\/b>CTG<\/span>
\nGTC\u2193<\/b>NNNGAC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Pvu II<\/td>\n
CAG\u2191<\/b>CTG<\/span>
\nGTC\u2193<\/b>GAC<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Rga I<\/td>\n
GCGAT\u2191<\/b>CGC<\/span>
\nCGC\u2193<\/b>TAGCG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
Rig I<\/td>\n
GGCCGG\u2191<\/b>CC<\/span>
\nCC\u2193<\/b>GGCCGG<\/span><\/center><\/td>\n
*<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n10<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Rsa I<\/td>\n
GT\u2191<\/b>AC<\/span>
\nCA\u2193<\/b>TG<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\nN<\/td>\n<\/tr>\n
RsaN I<\/td>\n
G\u2191<\/b>TAC<\/span>
\nCAT\u2193<\/b>G<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Rsr2 I<\/td>\n
CG\u2191<\/b>GWCCG<\/span>
\nGCCWG\u2193<\/b>GC<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Sal I<\/td>\n
G\u2191<\/b>TCGAC<\/span>
\nCAGCT\u2193<\/b>G<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n0 – 10<\/span><\/td>\n5<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Sbf I<\/td>\n
CCTGCA\u2191<\/b>GG<\/span>
\nGG\u2193<\/b>ACGTCC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Set I<\/td>\n
ASST\u2191<\/b><\/span>
\n\u2193<\/b>TSSA<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
SfaN I<\/td>\n
GCATC(N)5<\/sub>\u2191<\/b><\/span>
\nCGTAG(N)9<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n0 – 10<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Sfi I<\/td>\n
GGCCNNNN\u2191<\/b>NGGCC<\/span>
\nCCGGN\u2193<\/b>NNNNCCGG<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75<\/span><\/td>\n50<\/td>\n65<\/td>\n<\/tr>\n
Sfr274 I<\/td>\n
C\u2191<\/b>TCGAG<\/span>
\nGAGCT\u2193<\/b>C<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50<\/td>\n65<\/td>\n<\/tr>\n
Sfr303 I<\/td>\n
CCGC\u2191<\/b>GG<\/span>
\nGG\u2193<\/b>CGCC<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Sma I<\/td>\n
CCC\u2191<\/b>GGG<\/span>
\nGGG\u2193<\/b>CCC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n25<\/td>\n65<\/td>\n<\/tr>\n
Smi I<\/td>\n
ATTT\u2191<\/b>AAAT<\/span>
\nTAAA\u2193<\/b>TTTA<\/span><\/center><\/td>\n
O<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n10<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
SmiM I<\/td>\n
CAYNN\u2191<\/b>NNRTG<\/span>
\nGTRNN\u2193<\/b>NNYAC<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n10 – 25<\/span><\/td>\n60<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Sph I<\/td>\n
GCATG\u2191<\/b>C<\/span>
\nC\u2193<\/b>GTACG<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Sse9 I<\/td>\n
\u2191<\/b>AATT<\/span>
\nTTAA\u2193<\/b><\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75<\/span><\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n
Ssp I<\/td>\n
AAT\u2191<\/b>ATT<\/span>
\nTTA\u2193<\/b>TAA<\/span><\/center><\/td>\n
K<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Taq I<\/td>\n
T\u2191<\/b>CGA<\/span>
\nAGC\u2193<\/b>T<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
Tru9 I<\/td>\n
T\u2191<\/b>TAA<\/span>
\nAAT\u2193<\/b>T<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
TseF I<\/td>\n
\u2191<\/b>GTSAC<\/span>
\nCASTG\u2193<\/b><\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50<\/span><\/td>\n65<\/td>\nN<\/td>\n<\/tr>\n
Tth111 I<\/td>\n
GACN\u2191<\/b>NNGTC<\/span>
\nCTGNN\u2193<\/b>NCAG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
Vne I<\/td>\n
G\u2191<\/b>TGCAC<\/span>
\nCACGT\u2193<\/b>G<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Vsp I<\/td>\n
AT\u2191<\/b>TAAT<\/span>
\nTAAT\u2193<\/b>TA<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Xba I<\/td>\n
T\u2191<\/b>CTAGA<\/span>
\nAGATC\u2193<\/b>T<\/span><\/center><\/td>\n
O<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Xma I<\/td>\n
C\u2191<\/b>CCGGG<\/span>
\nGGGCC\u2193<\/b>C<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
ZraI<\/td>\n
GAC\u2191<\/b>GTC<\/span>
\nCTG\u2193<\/b>CAG<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Zrm I<\/td>\n
AGT\u2191<\/b>ACT<\/span>
\nTCA\u2193<\/b>TGA<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Zsp2 I<\/td>\n
ATGCA\u2191<\/b>T<\/span>
\nT\u2193<\/b>ACGTA<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

SE-buffers activity and inactivation temperature (20 minutes) Enzyme Recognition sequence Optimal buffer BSA % Activity Optimal temperature, \u00b0\u0421 Inactivation, \u00b0\u0421 B G O W Y Rose Aat II GACGT\u2191C C\u2193TGCAG Y – 10 – 25 25 – 50 10 – 25 25 – 50 100 50 37 65 Abs I CC\u2191TCGAGG GGAGCT\u2193CC * – 75 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-3026","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages\/3026","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/comments?post=3026"}],"version-history":[{"count":2,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages\/3026\/revisions"}],"predecessor-version":[{"id":4596,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages\/3026\/revisions\/4596"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=3026"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}