{"id":3026,"date":"2022-05-17T10:51:14","date_gmt":"2022-05-17T03:51:14","guid":{"rendered":"http:\/\/sibenzyme.com\/?page_id=3026"},"modified":"2026-09-03T15:51:45","modified_gmt":"2026-09-03T08:51:45","slug":"aktivity","status":"publish","type":"page","link":"https:\/\/sibenzyme.com\/en\/aktivity\/","title":{"rendered":"SE-buffers activity"},"content":{"rendered":"

Y<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAbs I<\/a><\/td>\n

5'\u2026 CC\u25bc<\/sup>TCGAGG \u20263'<\/div>
3'\u2026 GGAGCT\u25b2<\/sub>CC \u20265'<\/div><\/div><\/td>\nABSI<\/span>SE-buffer AbsI
\n10 mM Tris-HCl (pH 9.0 at 25\u00b0C); 10 mM MgCl2; 50 mM KCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n0 \u2013 10<\/td>\n25 \u2013 50<\/td>\n10 \u2013 25<\/td>\n25<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAcc16 I<\/a><\/td>\n
5'\u2026 TGC\u25bc<\/sup>GCA \u20263'<\/div>
3'\u2026 ACG\u25b2<\/sub>CGT \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n70<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAcc36 I<\/a><\/td>\n
5'\u2026 ACCTGC(N)4<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 TGGACG(N)8<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAcc65 I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>GTACC \u20263'<\/div>
3'\u2026 CCATG\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAccB1 I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>GYRCC \u20263'<\/div>
3'\u2026 CCRYG\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nK<\/span>SE-buffer K
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n30<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAccB7 I<\/a><\/td>\n
5'\u2026 CCANNNN\u25bc<\/sup>NTGG \u20263'<\/div>
3'\u2026 GGTN\u25b2<\/sub>NNNNACC \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAccBS I<\/a><\/td>\n
5'\u2026 GAG\u25bc<\/sup>CGG \u20263'<\/div>
3'\u2026 CTC\u25b2<\/sub>GCC \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAcl I<\/a><\/td>\n
5'\u2026 AA\u25bc<\/sup>CGTT \u20263'<\/div>
3'\u2026 TTGC\u25b2<\/sub>AA \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n80<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAclW I<\/a><\/td>\n
5'\u2026 GGATC(N)4<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CCTAG(N)5<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n30<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAco I<\/a><\/td>\n
5'\u2026 Y\u25bc<\/sup>GGCCR \u20263'<\/div>
3'\u2026 RCCGG\u25b2<\/sub>Y \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAcs I<\/a><\/td>\n
5'\u2026 R\u25bc<\/sup>AATTY \u20263'<\/div>
3'\u2026 YTTAA\u25b2<\/sub>R \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n\nAcu I<\/a><\/td>\n
5'\u2026 CTGAAG(N)16<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 GACTTC(N)14<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY + SAM<\/td>\n+<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAfe I<\/a><\/td>\n
5'\u2026 AGC\u25bc<\/sup>GCT \u20263'<\/div>
3'\u2026 TCG\u25b2<\/sub>CGA \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAgs I<\/a><\/td>\n
5'\u2026 TTS\u25bc<\/sup>AA \u20263'<\/div>
3'\u2026 AA\u25b2<\/sub>STT \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAhl I<\/a><\/td>\n
5'\u2026 A\u25bc<\/sup>CTAGT \u20263'<\/div>
3'\u2026 TGATC\u25b2<\/sub>A \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nAjn I<\/a><\/td>\n
5'\u2026 \u25bc<\/sup>CCWGG \u20263'<\/div>
3'\u2026 GGWCC\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n50<\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n\nAle I<\/a><\/td>\n
5'\u2026 CACNN\u25bc<\/sup>NNGTG \u20263'<\/div>
3'\u2026 GTGNN\u25b2<\/sub>NNCAC \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAlu I<\/a><\/td>\n
5'\u2026 AG\u25bc<\/sup>CT \u20263'<\/div>
3'\u2026 TC\u25b2<\/sub>GA \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAluB I<\/a><\/td>\n
5'\u2026 AG\u25bc<\/sup>CT \u20263'<\/div>
3'\u2026 TC\u25b2<\/sub>GA \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n75 \u2013 100<\/td>\n60<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAma87 I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>YCGRG \u20263'<\/div>
3'\u2026 GRGCY\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n0 \u2013 10<\/td>\n25<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAox I<\/a><\/td>\n
5'\u2026 \u25bc<\/sup>RG(5mC)Y \u20263'<\/div>
3'\u2026 Y(5mC)GR\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nAOXI<\/span>SE-buffer AoxI
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 3 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n60<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nApa I<\/a><\/td>\n
5'\u2026 GGGCC\u25bc<\/sup>C \u20263'<\/div>
3'\u2026 C\u25b2<\/sub>CCGGG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nArs I<\/a><\/td>\n
5'\u2026 \u25bc<\/sup>(N)8<\/sub>GACNNNNNNTTYG(N)11<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 \u25b2<\/sub>(N)13<\/sub>CTGNNNNNNAARC(N)6<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n20<\/td>\n30<\/td>\n65<\/td>\n<\/tr>\n\nAsiG I<\/a><\/td>\n
5'\u2026 A\u25bc<\/sup>CCGGT \u20263'<\/div>
3'\u2026 TGGCC\u25b2<\/sub>A \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n75<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAsiS I<\/a><\/td>\n
5'\u2026 GCGAT\u25bc<\/sup>CGC \u20263'<\/div>
3'\u2026 CGC\u25b2<\/sub>TAGCG \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n25<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nAspA2 I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>CTAGG \u20263'<\/div>
3'\u2026 GGATC\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n10<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nAspLE I<\/a><\/td>\n
5'\u2026 GCG\u25bc<\/sup>C \u20263'<\/div>
3'\u2026 C\u25b2<\/sub>GCG \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n37<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nAspS9 I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>GNCC \u20263'<\/div>
3'\u2026 CCNG\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n75<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAsuC2 I<\/a><\/td>\n
5'\u2026 CC\u25bc<\/sup>SGG \u20263'<\/div>
3'\u2026 GGS\u25b2<\/sub>CC \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n40<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAsuHP I<\/a><\/td>\n
5'\u2026 GGTGA(N)8<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CCACT(N)7<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nAsuNH I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>CTAGC \u20263'<\/div>
3'\u2026 CGATC\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n25<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBamH I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>GATCC \u20263'<\/div>
3'\u2026 CCTAG\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBar I<\/a><\/td>\n
5'\u2026 \u25bc<\/sup>(N)7<\/sub>GAAGNNNNNNTAC(N)12<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 \u25b2<\/sub>(N)12<\/sub>CTTCNNNNNNATG(N)7<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\n2K<\/span>SE-buffer 2K
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n40<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBbv12 I<\/a><\/td>\n
5'\u2026 GWGCW\u25bc<\/sup>C \u20263'<\/div>
3'\u2026 C\u25b2<\/sub>WCGWG \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n60<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nBgl I<\/a><\/td>\n
5'\u2026 GCCNNNN\u25bc<\/sup>NGGC \u20263'<\/div>
3'\u2026 CGGN\u25b2<\/sub>NNNNCCG \u20265'<\/div><\/div><\/td>\n2W<\/span>SE-buffer 2W
\n20 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 200 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n0 \u2013 10<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBgl II<\/a><\/td>\n
5'\u2026 A\u25bc<\/sup>GATCT \u20263'<\/div>
3'\u2026 TCTAG\u25b2<\/sub>A \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nBls I<\/a><\/td>\n\u041d\u0435\u0432\u0435\u0440\u043d\u044b\u0439 \u0444\u043e\u0440\u043c\u0430\u0442 \u0441\u0430\u0439\u0442\u0430 \u0443\u0437\u043d\u0430\u0432\u0430\u043d\u0438\u044f<\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n50<\/td>\n30<\/td>\n65<\/td>\n<\/tr>\n\nBme18 I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>GWCC \u20263'<\/div>
3'\u2026 CCWG\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n80<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBmo I<\/a><\/td>\n
5'\u2026 GG\u25bc<\/sup>GWCCC \u20263'<\/div>
3'\u2026 CCCWG\u25b2<\/sub>GG \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n75<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBmt I<\/a><\/td>\n
5'\u2026 GCTAG\u25bc<\/sup>C \u20263'<\/div>
3'\u2026 C\u25b2<\/sub>GATCG \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBmu I<\/a><\/td>\n
5'\u2026 ACTGGG(N)5<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 TGACCC(N)4<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n25<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBpm I<\/a><\/td>\n
5'\u2026 CTGGAG(N)16<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 GACCTC(N)14<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBpu10 I<\/a><\/td>\n
5'\u2026 CC\u25bc<\/sup>TNAGC \u20263'<\/div>
3'\u2026 GGANT\u25b2<\/sub>CG \u20265'<\/div><\/div><\/td>\nK<\/span>SE-buffer K
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nBpu14 I<\/a><\/td>\n
5'\u2026 TT\u25bc<\/sup>CGAA \u20263'<\/div>
3'\u2026 AAGC\u25b2<\/sub>TT \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBsa29 I<\/a><\/td>\n
5'\u2026 AT\u25bc<\/sup>CGAT \u20263'<\/div>
3'\u2026 TAGC\u25b2<\/sub>TA \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBsc4 I<\/a><\/td>\n
5'\u2026 CCNNNNN\u25bc<\/sup>NNGG \u20263'<\/div>
3'\u2026 GGNN\u25b2<\/sub>NNNNNCC \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n90<\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n\nBse1 I<\/a><\/td>\n
5'\u2026 ACTGGN\u25bc<\/sup> \u20263'<\/div>
3'\u2026 TGAC\u25b2<\/sub>CN \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n100<\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n\nBse118 I<\/a><\/td>\n
5'\u2026 R\u25bc<\/sup>CCGGY \u20263'<\/div>
3'\u2026 YGGCC\u25b2<\/sub>R \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n0 \u2013 10<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n\nBse21 I<\/a><\/td>\n
5'\u2026 CC\u25bc<\/sup>TNAGG \u20263'<\/div>
3'\u2026 GGANT\u25b2<\/sub>CC \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n40<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nBse3D I<\/a><\/td>\n
5'\u2026 GCAATGNN\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CGTTAC\u25b2<\/sub>NN \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n\nBse8 I<\/a><\/td>\n
5'\u2026 GATNN\u25bc<\/sup>NNATC \u20263'<\/div>
3'\u2026 CTANN\u25b2<\/sub>NNTAG \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n\nBseP I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>CGCGC \u20263'<\/div>
3'\u2026 CGCGC\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n50<\/td>\n65<\/td>\n<\/tr>\n\nBseX3 I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>GGCCG \u20263'<\/div>
3'\u2026 GCCGG\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n25<\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n\nBslF I<\/a><\/td>\n
5'\u2026 GGGAC(N)10<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CCCTG(N)14<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/td>\n+<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n50<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nBso31 I<\/a><\/td>\n
5'\u2026 GGTCTCN\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CCAGAG(N)5<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n40<\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n\nBsp13 I<\/a><\/td>\n
5'\u2026 T\u25bc<\/sup>CCGGA \u20263'<\/div>
3'\u2026 AGGCC\u25b2<\/sub>T \u20265'<\/div><\/div><\/td>\n2K<\/span>SE-buffer 2K
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n0 \u2013 10<\/td>\n5<\/td>\n50<\/td>\n65<\/td>\n<\/tr>\n\nBsp1720 I<\/a><\/td>\n
5'\u2026 GC\u25bc<\/sup>TNAGC \u20263'<\/div>
3'\u2026 CGANT\u25b2<\/sub>CG \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n75<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nBsp19 I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>CATGG \u20263'<\/div>
3'\u2026 GGTAC\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\n2W<\/span>SE-buffer 2W
\n20 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 200 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n5<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBspAC I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>CGC \u20263'<\/div>
3'\u2026 GGC\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBspFN I<\/a><\/td>\n
5'\u2026 CG\u25bc<\/sup>CG \u20263'<\/div>
3'\u2026 GC\u25b2<\/sub>GC \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBssEC I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>CNNGG \u20263'<\/div>
3'\u2026 GGNNC\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n100<\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n\nBssNA I<\/a><\/td>\n
5'\u2026 GTA\u25bc<\/sup>TAC \u20263'<\/div>
3'\u2026 CAT\u25b2<\/sub>ATG \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nBssT1 I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>CWWGG \u20263'<\/div>
3'\u2026 GGWWC\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\n2K<\/span>SE-buffer 2K
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n\nBst2B I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>TCGTG \u20263'<\/div>
3'\u2026 GAGCA\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n\nBst2U I<\/a><\/td>\n
5'\u2026 CC\u25bc<\/sup>WGG \u20263'<\/div>
3'\u2026 GGW\u25b2<\/sub>CC \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n50<\/td>\n60<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nBst4C I<\/a><\/td>\n
5'\u2026 ACN\u25bc<\/sup>GT \u20263'<\/div>
3'\u2026 TG\u25b2<\/sub>NCA \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n50<\/td>\n65<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nBst6 I<\/a><\/td>\n
5'\u2026 CTCTTCN\u25bc<\/sup> \u20263'<\/div>
3'\u2026 GAGAAG(N)4<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n100<\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n\nBstAC I<\/a><\/td>\n
5'\u2026 GR\u25bc<\/sup>CGYC \u20263'<\/div>
3'\u2026 CYGC\u25b2<\/sub>RG \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nBstAF I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>TTAAG \u20263'<\/div>
3'\u2026 GAATT\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n\nBstAP I<\/a><\/td>\n
5'\u2026 GCANNNN\u25bc<\/sup>NTGC \u20263'<\/div>
3'\u2026 CGTN\u25b2<\/sub>NNNNACG \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n\nBstAU I<\/a><\/td>\n
5'\u2026 T\u25bc<\/sup>GTACA \u20263'<\/div>
3'\u2026 ACATG\u25b2<\/sub>T \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nBstBA I<\/a><\/td>\n
5'\u2026 YAC\u25bc<\/sup>GTR \u20263'<\/div>
3'\u2026 RTG\u25b2<\/sub>CAY \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n50<\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n\nBstC8 I<\/a><\/td>\n
5'\u2026 GCN\u25bc<\/sup>NGC \u20263'<\/div>
3'\u2026 CGN\u25b2<\/sub>NCG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n100<\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n\nBstDE I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>TNAG \u20263'<\/div>
3'\u2026 GANT\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n\nBstDS I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>CRYGG \u20263'<\/div>
3'\u2026 GGYRC\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n0 \u2013 10<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n\nBstEN I<\/a><\/td>\n
5'\u2026 CCTNN\u25bc<\/sup>NNNAGG \u20263'<\/div>
3'\u2026 GGANNN\u25b2<\/sub>NNTCC \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n\nBstF5 I<\/a><\/td>\n
5'\u2026 GGATGNN\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CCTAC\u25b2<\/sub>NN \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n100<\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n\nBstFN I<\/a><\/td>\n
5'\u2026 CG\u25bc<\/sup>CG \u20263'<\/div>
3'\u2026 GC\u25b2<\/sub>GC \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n75<\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n\nBstH2 I<\/a><\/td>\n
5'\u2026 RGCGC\u25bc<\/sup>Y \u20263'<\/div>
3'\u2026 Y\u25b2<\/sub>CGCGR \u20265'<\/div><\/div><\/td>\nY<\/td>\n+<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n100<\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n\nBstHH I<\/a><\/td>\n
5'\u2026 GCG\u25bc<\/sup>C \u20263'<\/div>
3'\u2026 C\u25b2<\/sub>GCG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n100<\/td>\n50<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nBstKT I<\/a><\/td>\n
5'\u2026 GAT\u25bc<\/sup>C \u20263'<\/div>
3'\u2026 C\u25b2<\/sub>TAG \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBstMA I<\/a><\/td>\n
5'\u2026 GTCTCN\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CAGAG(N)5<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n\nBstMB I<\/a><\/td>\n
5'\u2026 \u25bc<\/sup>GATC \u20263'<\/div>
3'\u2026 CTAG\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n\nBstMC I<\/a><\/td>\n
5'\u2026 CGRY\u25bc<\/sup>CG \u20263'<\/div>
3'\u2026 GC\u25b2<\/sub>YRGC \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n\nBstMW I<\/a><\/td>\n
5'\u2026 GCNNNNN\u25bc<\/sup>NNGC \u20263'<\/div>
3'\u2026 CGNN\u25b2<\/sub>NNNNNCG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n40<\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n\nBstNS I<\/a><\/td>\n
5'\u2026 RCATG\u25bc<\/sup>Y \u20263'<\/div>
3'\u2026 Y\u25b2<\/sub>GTACR \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n75 \u2013 100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBstPA I<\/a><\/td>\n
5'\u2026 GACNN\u25bc<\/sup>NNGTC \u20263'<\/div>
3'\u2026 CTGNN\u25b2<\/sub>NNCAG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n100<\/td>\n65<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nBstSC I<\/a><\/td>\n
5'\u2026 \u25bc<\/sup>CCNGG \u20263'<\/div>
3'\u2026 GGNCC\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n60<\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n\nBstSF I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>TRYAG \u20263'<\/div>
3'\u2026 GAYRT\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n60<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nBstSL I<\/a><\/td>\n
5'\u2026 GKGCm\u25bc<\/sup>C \u20263'<\/div>
3'\u2026 C\u25b2<\/sub>mCGKG \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n\nBstSN I<\/a><\/td>\n
5'\u2026 TAC\u25bc<\/sup>GTA \u20263'<\/div>
3'\u2026 ATG\u25b2<\/sub>CAT \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n50<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nBstV1 I<\/a><\/td>\n
5'\u2026 GCAGC(N)8<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CGTCG(N)12<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n\nBstV2 I<\/a><\/td>\n
5'\u2026 GAAGAC(N)2<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CTTCTG(N)6<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n70<\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n\nBstX I<\/a><\/td>\n
5'\u2026 CCANNNNN\u25bc<\/sup>NTGG \u20263'<\/div>
3'\u2026 GGTN\u25b2<\/sub>NNNNNACC \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBstX2 I<\/a><\/td>\n
5'\u2026 R\u25bc<\/sup>GATCY \u20263'<\/div>
3'\u2026 YCTAG\u25b2<\/sub>R \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n\nBsu I<\/a><\/td>\n
5'\u2026 GTATCC(N)6<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CATAGG(N)5<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n10<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nBsuR I<\/a><\/td>\n
5'\u2026 GG\u25bc<\/sup>CC \u20263'<\/div>
3'\u2026 CC\u25b2<\/sub>GG \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nBtr I<\/a><\/td>\n
5'\u2026 CAC\u25bc<\/sup>GTC \u20263'<\/div>
3'\u2026 GTG\u25b2<\/sub>CAG \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n\nCci I<\/a><\/td>\n
5'\u2026 T\u25bc<\/sup>CATGA \u20263'<\/div>
3'\u2026 AGTAC\u25b2<\/sub>T \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n\nCciN I<\/a><\/td>\n
5'\u2026 GC\u25bc<\/sup>GGCCGC \u20263'<\/div>
3'\u2026 CGCCGG\u25b2<\/sub>CG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nDra I<\/a><\/td>\n
5'\u2026 TTT\u25bc<\/sup>AAA \u20263'<\/div>
3'\u2026 AAA\u25b2<\/sub>TTT \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nDra III<\/a><\/td>\n
5'\u2026 CACNNN\u25bc<\/sup>GTG \u20263'<\/div>
3'\u2026 GTG\u25b2<\/sub>NNNCAC \u20265'<\/div><\/div><\/td>\n2K<\/span>SE-buffer 2K
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nDri I<\/a><\/td>\n
5'\u2026 GACNNN\u25bc<\/sup>NNGTC \u20263'<\/div>
3'\u2026 CTGNN\u25b2<\/sub>NNNCAG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n40<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nDseD I<\/a><\/td>\n
5'\u2026 GACNNNN\u25bc<\/sup>NNGTC \u20263'<\/div>
3'\u2026 CTGNN\u25b2<\/sub>NNNNCAG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n30<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nEcoICR I<\/a><\/td>\n
5'\u2026 GAG\u25bc<\/sup>CTC \u20263'<\/div>
3'\u2026 CTC\u25b2<\/sub>GAG \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n75 \u2013 100<\/td>\n5<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nEcoR I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>AATTC \u20263'<\/div>
3'\u2026 CTTAA\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nECORI<\/span>SE-buffer EcoRI
\n100 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nEcoR V<\/a><\/td>\n
5'\u2026 GAT\u25bc<\/sup>ATC \u20263'<\/div>
3'\u2026 CTA\u25b2<\/sub>TAG \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n0 \u2013 10<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n50<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nEge I<\/a><\/td>\n
5'\u2026 GGC\u25bc<\/sup>GCC \u20263'<\/div>
3'\u2026 CCG\u25b2<\/sub>CGG \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nEmiS6 I<\/a><\/td>\n
5'\u2026 CTCTTC(N)1<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 GAGAAG(N)4<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n25<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nErh I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>CWWGG \u20263'<\/div>
3'\u2026 GGWWC\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\n2W<\/span>SE-buffer 2W
\n20 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 200 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nFae I<\/a><\/td>\n
5'\u2026 CATG\u25bc<\/sup> \u20263'<\/div>
3'\u2026 \u25b2<\/sub>GTAC \u20265'<\/div><\/div><\/td>\nFAEI<\/span>SE-buffer FaeI
\n33 mM Tris-acetate (pH 8.3 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nFai I<\/a><\/td>\n
5'\u2026 YA\u25bc<\/sup>TR \u20263'<\/div>
3'\u2026 RT\u25b2<\/sub>AY \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n\nFal I<\/a><\/td>\n
5'\u2026 \u25bc<\/sup>(N)8<\/sub>AAGNNNNNCTT(N)13<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 \u25b2<\/sub>(N)13<\/sub>TTCNNNNNGAA(N)8<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n0 \u2013 10<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n70<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nFat I<\/a><\/td>\n
5'\u2026 \u25bc<\/sup>CATG \u20263'<\/div>
3'\u2026 GTAC\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n\nFau I<\/a><\/td>\n
5'\u2026 CCCGC(N)4<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 GGGCG(N)6<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n50 \u2013 75<\/td>\n50<\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n\nFauND I<\/a><\/td>\n
5'\u2026 CA\u25bc<\/sup>TATG \u20263'<\/div>
3'\u2026 GTAT\u25b2<\/sub>AC \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nFbl I<\/a><\/td>\n
5'\u2026 GT\u25bc<\/sup>mKAC \u20263'<\/div>
3'\u2026 CAKm\u25b2<\/sub>TG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n0 \u2013 10<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n100<\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n\nFok I<\/a><\/td>\n
5'\u2026 GGATG(N)9<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CCTAC(N)13<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nFriO I<\/a><\/td>\n
5'\u2026 GRGCY\u25bc<\/sup>C \u20263'<\/div>
3'\u2026 C\u25b2<\/sub>YCGRG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n25<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nFsp4H I<\/a><\/td>\n
5'\u2026 GC\u25bc<\/sup>NGC \u20263'<\/div>
3'\u2026 CGN\u25b2<\/sub>CG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nGla I<\/a><\/td>\n
5'\u2026 R(5mC)\u25bc<\/sup>GY \u20263'<\/div>
3'\u2026 YG\u25b2<\/sub>(5mC)R \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n30<\/td>\n65<\/td>\n<\/tr>\n\nGlu I<\/a><\/td>\n
5'\u2026 G(5mC)\u25bc<\/sup>NG(5mC) \u20263'<\/div>
3'\u2026 (5mC)GN\u25b2<\/sub>(5mC)G \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nGsa I<\/a><\/td>\n
5'\u2026 CCCAG\u25bc<\/sup>C \u20263'<\/div>
3'\u2026 G\u25b2<\/sub>GGTCG \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n70<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nHae III<\/a><\/td>\n
5'\u2026 GG\u25bc<\/sup>CC \u20263'<\/div>
3'\u2026 CC\u25b2<\/sub>GG \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nHga I<\/a><\/td>\n
5'\u2026 GACGC(N)5<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CTGCG(N)10<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nHind II<\/a><\/td>\n
5'\u2026 GTY\u25bc<\/sup>RAC \u20263'<\/div>
3'\u2026 CAR\u25b2<\/sub>YTG \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nHind III<\/a><\/td>\n
5'\u2026 A\u25bc<\/sup>AGCTT \u20263'<\/div>
3'\u2026 TTCGA\u25b2<\/sub>A \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nHinf I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>ANTC \u20263'<\/div>
3'\u2026 CTNA\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nHpa I<\/a><\/td>\n
5'\u2026 GTT\u25bc<\/sup>AAC \u20263'<\/div>
3'\u2026 CAA\u25b2<\/sub>TTG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n0 \u2013 10<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n25<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nHpa II<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>CGG \u20263'<\/div>
3'\u2026 GGC\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n50<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nHpySE526 I<\/a><\/td>\n
5'\u2026 A\u25bc<\/sup>CGT \u20263'<\/div>
3'\u2026 TGC\u25b2<\/sub>A \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nHspA I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>CGC \u20263'<\/div>
3'\u2026 CGC\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nKpn I<\/a><\/td>\n
5'\u2026 GGTAC\u25bc<\/sup>C \u20263'<\/div>
3'\u2026 C\u25b2<\/sub>CATGG \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n50<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nKro I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>C(5mC)GGC \u20263'<\/div>
3'\u2026 CGG(5mC)C\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nKroN I<\/a><\/td>\n
5'\u2026 GCC\u25bc<\/sup>GGC \u20263'<\/div>
3'\u2026 CGG\u25b2<\/sub>CCG \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n50<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nKsp22 I<\/a><\/td>\n
5'\u2026 T\u25bc<\/sup>GATCA \u20263'<\/div>
3'\u2026 ACTAG\u25b2<\/sub>T \u20265'<\/div><\/div><\/td>\n2K<\/span>SE-buffer 2K
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nKzo9 I<\/a><\/td>\n
5'\u2026 \u25bc<\/sup>GATC \u20263'<\/div>
3'\u2026 CTAG\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nLmn I<\/a><\/td>\n
5'\u2026 GCTCCN\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CGAG\u25b2<\/sub>GN \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n80<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nMab I<\/a><\/td>\n
5'\u2026 A\u25bc<\/sup>CCWGGT \u20263'<\/div>
3'\u2026 TGGWCC\u25b2<\/sub>A \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nMal I<\/a><\/td>\n
5'\u2026 G(mA)\u25bc<\/sup>TC \u20263'<\/div>
3'\u2026 CT\u25b2<\/sub>(mA)G \u20265'<\/div><\/div><\/td>\nMALI<\/span>SE-buffer MalI
\n20 mM Tris-HCl (pH 9.0 at 25\u00b0C); 10 mM MgCl2; 150 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nMbo II<\/a><\/td>\n
5'\u2026 GAAGA(N)8<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CTTCT(N)7<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nMfe I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>AATTG \u20263'<\/div>
3'\u2026 GTTAA\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n25<\/td>\n37<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nMhl I<\/a><\/td>\n
5'\u2026 GDGCH\u25bc<\/sup>C \u20263'<\/div>
3'\u2026 C\u25b2<\/sub>HCGDG \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nMlu I<\/a><\/td>\n
5'\u2026 A\u25bc<\/sup>CGCGT \u20263'<\/div>
3'\u2026 TGCGC\u25b2<\/sub>A \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n10 \u2013 25<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nMly113 I<\/a><\/td>\n
5'\u2026 GG\u25bc<\/sup>CGCC \u20263'<\/div>
3'\u2026 CCGC\u25b2<\/sub>GG \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nMnl I<\/a><\/td>\n
5'\u2026 CCTC(N)7<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 GGAG(N)6<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nMox20 I<\/a><\/td>\n
5'\u2026 TGG\u25bc<\/sup>CCA \u20263'<\/div>
3'\u2026 ACC\u25b2<\/sub>GGT \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n75<\/td>\n37<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nMroN I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>CCGGC \u20263'<\/div>
3'\u2026 CGGCC\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n5<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nMroX I<\/a><\/td>\n
5'\u2026 GAANN\u25bc<\/sup>NNTTC \u20263'<\/div>
3'\u2026 CTTNN\u25b2<\/sub>NNAAG \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nMsp I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>CGG \u20263'<\/div>
3'\u2026 GGC\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nMspA1 I<\/a><\/td>\n
5'\u2026 CmG\u25bc<\/sup>CKG \u20263'<\/div>
3'\u2026 GKC\u25b2<\/sub>GmC \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n10 \u2013 25<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nMspR9 I<\/a><\/td>\n
5'\u2026 CC\u25bc<\/sup>NGG \u20263'<\/div>
3'\u2026 GGN\u25b2<\/sub>CC \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nMte I<\/a><\/td>\n
5'\u2026 G(5mC)G(5mC)\u25bc<\/sup>NG(5mC)G(5mC) \u20263'<\/div>
3'\u2026 (5mC)G(5mC)GN\u25b2<\/sub>(5mC)G(5mC)G \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n55<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nNru I<\/a><\/td>\n
5'\u2026 TCG\u25bc<\/sup>CGA \u20263'<\/div>
3'\u2026 AGC\u25b2<\/sub>GCT \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nPalA I<\/a><\/td>\n
5'\u2026 GG\u25bc<\/sup>CGCGCC \u20263'<\/div>
3'\u2026 CCGCGC\u25b2<\/sub>GG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n40<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPce I<\/a><\/td>\n
5'\u2026 AGG\u25bc<\/sup>CCT \u20263'<\/div>
3'\u2026 TCC\u25b2<\/sub>GGA \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n\nPci I<\/a><\/td>\n
5'\u2026 A\u25bc<\/sup>CATGT \u20263'<\/div>
3'\u2026 TGTAC\u25b2<\/sub>A \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPciS I<\/a><\/td>\n
5'\u2026 GCTCTTCN\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CGAGAAG(N)4<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n75 \u2013 100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPcs I<\/a><\/td>\n
5'\u2026 (5mC)GNNNNN\u25bc<\/sup>NN(5mC)G \u20263'<\/div>
3'\u2026 G(5mC)NN\u25b2<\/sub>NNNNNG(5mC) \u20265'<\/div><\/div><\/td>\n3M<\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPct I<\/a><\/td>\n
5'\u2026 GAATGCN\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CTTAC\u25b2<\/sub>GN \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPec I<\/a><\/td>\n
5'\u2026 TTA\u25bc<\/sup>TAA \u20263'<\/div>
3'\u2026 AAT\u25b2<\/sub>ATT \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPkr I<\/a><\/td>\n
5'\u2026 G(5mC)N\u25bc<\/sup>G(5mC) \u20263'<\/div>
3'\u2026 (5mC)G\u25b2<\/sub>N(5mC)G \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPle19 I<\/a><\/td>\n
5'\u2026 CGAT\u25bc<\/sup>CG \u20263'<\/div>
3'\u2026 GC\u25b2<\/sub>TAGC \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPps I<\/a><\/td>\n
5'\u2026 GAGTC(N)4<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CTCAG(N)5<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n0 \u2013 10<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPse31 I<\/a><\/td>\n
5'\u2026 GGTCTC(N)1<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CCAGAG(N)5<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n75<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPsi I<\/a><\/td>\n
5'\u2026 TTA\u25bc<\/sup>TAA \u20263'<\/div>
3'\u2026 AAT\u25b2<\/sub>ATT \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n40<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPsp124B I<\/a><\/td>\n
5'\u2026 GAGCT\u25bc<\/sup>C \u20263'<\/div>
3'\u2026 C\u25b2<\/sub>TCGAG \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n10 \u2013 25<\/td>\n0 \u2013 10<\/td>\n75 \u2013 100<\/td>\n30<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nPsp6 I<\/a><\/td>\n
5'\u2026 \u25bc<\/sup>CCWGG \u20263'<\/div>
3'\u2026 GGWCC\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n10<\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n\nPspC I<\/a><\/td>\n
5'\u2026 CAC\u25bc<\/sup>GTG \u20263'<\/div>
3'\u2026 GTG\u25b2<\/sub>CAC \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n50 \u2013 75<\/td>\n5<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPspE I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>GTNACC \u20263'<\/div>
3'\u2026 CCANTG\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPspL I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>GTACG \u20263'<\/div>
3'\u2026 GCATG\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPspN4 I<\/a><\/td>\n
5'\u2026 GGN\u25bc<\/sup>NCC \u20263'<\/div>
3'\u2026 CCN\u25b2<\/sub>NGG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPspOM I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>GGCCC \u20263'<\/div>
3'\u2026 CCCGG\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n10 \u2013 25<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n25<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPspPP I<\/a><\/td>\n
5'\u2026 RG\u25bc<\/sup>GWCCY \u20263'<\/div>
3'\u2026 YCCWG\u25b2<\/sub>GR \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPspX I<\/a><\/td>\n
5'\u2026 VC\u25bc<\/sup>TCGAGB \u20263'<\/div>
3'\u2026 BGAGCT\u25b2<\/sub>CV \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n25<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nPsr I<\/a><\/td>\n
5'\u2026 \u25bc<\/sup>(N)7<\/sub>GAACNNNNNNTAC(N)12<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 \u25b2<\/sub>(N)12<\/sub>CTTGNNNNNNATG(N)7<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n30<\/td>\n30<\/td>\n65<\/td>\n<\/tr>\n\nPst I<\/a><\/td>\n
5'\u2026 CTGCA\u25bc<\/sup>G \u20263'<\/div>
3'\u2026 G\u25b2<\/sub>ACGTC \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n50<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nPstN I<\/a><\/td>\n
5'\u2026 CAGNNN\u25bc<\/sup>CTG \u20263'<\/div>
3'\u2026 GTC\u25b2<\/sub>NNNGAC \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nPvu II<\/a><\/td>\n
5'\u2026 CAG\u25bc<\/sup>CTG \u20263'<\/div>
3'\u2026 GTC\u25b2<\/sub>GAC \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nRga I<\/a><\/td>\n
5'\u2026 GCGAT\u25bc<\/sup>CGC \u20263'<\/div>
3'\u2026 CGC\u25b2<\/sub>TAGCG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n100<\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n\nRig I<\/a><\/td>\n
5'\u2026 GGCCGG\u25bc<\/sup>CC \u20263'<\/div>
3'\u2026 CC\u25b2<\/sub>GGCCGG \u20265'<\/div><\/div><\/td>\nRIGI<\/span>SE-buffer RigI
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 5 mM MgCl2; 1mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n10<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nRsa I<\/a><\/td>\n
5'\u2026 GT\u25bc<\/sup>AC \u20263'<\/div>
3'\u2026 CA\u25b2<\/sub>TG \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n0 \u2013 10<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n50<\/td>\n37<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nRsaN I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>TAC \u20263'<\/div>
3'\u2026 CAT\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nRsr2 I<\/a><\/td>\n
5'\u2026 CG\u25bc<\/sup>GWCCG \u20263'<\/div>
3'\u2026 GCCWG\u25b2<\/sub>GC \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n25<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nSal I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>TCGAC \u20263'<\/div>
3'\u2026 CAGCT\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n0 \u2013 10<\/td>\n5<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nSbf I<\/a><\/td>\n
5'\u2026 CCTGCA\u25bc<\/sup>GG \u20263'<\/div>
3'\u2026 GG\u25b2<\/sub>ACGTCC \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n50<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nSet I<\/a><\/td>\n
5'\u2026 ASST\u25bc<\/sup> \u20263'<\/div>
3'\u2026 \u25b2<\/sub>TSSA \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n100<\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n\nSfaN I<\/a><\/td>\n
5'\u2026 GCATC(N)5<\/sub>\u25bc<\/sup> \u20263'<\/div>
3'\u2026 CGTAG(N)9<\/sub>\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n0 \u2013 10<\/td>\n25<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nSfi I<\/a><\/td>\n
5'\u2026 GGCCNNNN\u25bc<\/sup>NGGCC \u20263'<\/div>
3'\u2026 CCGGN\u25b2<\/sub>NNNNCCGG \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n75<\/td>\n50<\/td>\n65<\/td>\n<\/tr>\n\nSfr274 I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>TCGAG \u20263'<\/div>
3'\u2026 GAGCT\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n50<\/td>\n65<\/td>\n<\/tr>\n\nSfr303 I<\/a><\/td>\n
5'\u2026 CCGC\u25bc<\/sup>GG \u20263'<\/div>
3'\u2026 GG\u25b2<\/sub>CGCC \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nSma I<\/a><\/td>\n
5'\u2026 CCC\u25bc<\/sup>GGG \u20263'<\/div>
3'\u2026 GGG\u25b2<\/sub>CCC \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n50<\/td>\n25<\/td>\n65<\/td>\n<\/tr>\n\nSmi I<\/a><\/td>\n
5'\u2026 ATTT\u25bc<\/sup>AAAT \u20263'<\/div>
3'\u2026 TAAA\u25b2<\/sub>TTTA \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n10<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nSmiM I<\/a><\/td>\n
5'\u2026 CAYNN\u25bc<\/sup>NNRTG \u20263'<\/div>
3'\u2026 GTRNN\u25b2<\/sub>NNYAC \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n10 \u2013 25<\/td>\n60<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nSph I<\/a><\/td>\n
5'\u2026 GCATG\u25bc<\/sup>C \u20263'<\/div>
3'\u2026 C\u25b2<\/sub>GTACG \u20265'<\/div><\/div><\/td>\nG<\/span>SE-buffer G
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nSse9 I<\/a><\/td>\n
5'\u2026 \u25bc<\/sup>AATT \u20263'<\/div>
3'\u2026 TTAA\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n75<\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n\nSsp I<\/a><\/td>\n
5'\u2026 AAT\u25bc<\/sup>ATT \u20263'<\/div>
3'\u2026 TTA\u25b2<\/sub>TAA \u20265'<\/div><\/div><\/td>\nK<\/span>SE-buffer K
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nSspM I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>TAG \u20263'<\/div>
3'\u2026 GAT\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n80<\/td>\n55<\/td>\n\u0424\u0435\u0440\u043c\u0435\u043d\u0442 \u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f \u043f\u0440\u043e\u0433\u0440\u0435\u0432\u0430\u043d\u0438\u0435\u043c \u043f\u0440\u0438 80\u00b0\u0421 \u0432 \u0442\u0435\u0447\u0435\u043d\u0438\u0435 20 \u043c\u0438\u043d\u0443\u0442<\/td>\n<\/tr>\n\nTaq I<\/a><\/td>\n
5'\u2026 T\u25bc<\/sup>CGA \u20263'<\/div>
3'\u2026 AGC\u25b2<\/sub>T \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n100<\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n\nTru9 I<\/a><\/td>\n
5'\u2026 T\u25bc<\/sup>TAA \u20263'<\/div>
3'\u2026 AAT\u25b2<\/sub>T \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n\nTseF I<\/a><\/td>\n
5'\u2026 \u25bc<\/sup>GTSAC \u20263'<\/div>
3'\u2026 CASTG\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n0 \u2013 10<\/td>\n25 \u2013 50<\/td>\n50 \u2013 75<\/td>\n50<\/td>\n65<\/td>\n\u043d\u0435 \u0438\u043d\u0430\u043a\u0442\u0438\u0432\u0438\u0440\u0443\u0435\u0442\u0441\u044f<\/td>\n<\/tr>\n\nTth111 I<\/a><\/td>\n
5'\u2026 GACN\u25bc<\/sup>NNGTC \u20263'<\/div>
3'\u2026 CTGNN\u25b2<\/sub>NCAG \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n10 \u2013 25<\/td>\n10 \u2013 25<\/td>\n100<\/td>\n100<\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n\nVne I<\/a><\/td>\n
5'\u2026 G\u25bc<\/sup>TGCAC \u20263'<\/div>
3'\u2026 CACGT\u25b2<\/sub>G \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n10 \u2013 25<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n100<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nVsp I<\/a><\/td>\n
5'\u2026 AT\u25bc<\/sup>TAAT \u20263'<\/div>
3'\u2026 TAAT\u25b2<\/sub>TA \u20265'<\/div><\/div><\/td>\nW<\/span>SE-buffer W
\n10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n0 \u2013 10<\/td>\n10 \u2013 25<\/td>\n50 \u2013 75<\/td>\n100<\/td>\n25 \u2013 50<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nXba I<\/a><\/td>\n
5'\u2026 T\u25bc<\/sup>CTAGA \u20263'<\/div>
3'\u2026 AGATC\u25b2<\/sub>T \u20265'<\/div><\/div><\/td>\nO<\/span>SE-buffer O
\n50 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n75 \u2013 100<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n75 \u2013 100<\/td>\n25<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nXma I<\/a><\/td>\n
5'\u2026 C\u25bc<\/sup>CCGGG \u20263'<\/div>
3'\u2026 GGGCC\u25b2<\/sub>C \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n75 \u2013 100<\/td>\n50 \u2013 75<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nZra I<\/a><\/td>\n
5'\u2026 GAC\u25bc<\/sup>GTC \u20263'<\/div>
3'\u2026 CTG\u25b2<\/sub>CAG \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n\u2013<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n75 \u2013 100<\/td>\n100<\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n\nZrm I<\/a><\/td>\n
5'\u2026 AGT\u25bc<\/sup>ACT \u20263'<\/div>
3'\u2026 TCA\u25b2<\/sub>TGA \u20265'<\/div><\/div><\/td>\nY<\/span>SE-buffer Y
\n33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n0 \u2013 10<\/td>\n0 \u2013 10<\/td>\n100<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n\nZsp2 I<\/a><\/td>\n
5'\u2026 ATGCA\u25bc<\/sup>T \u20263'<\/div>
3'\u2026 T\u25b2<\/sub>ACGTA \u20265'<\/div><\/div><\/td>\nB<\/span>SE-buffer B
\n10 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/span><\/span><\/td>\n+<\/td>\n100<\/td>\n50 \u2013 75<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n25 \u2013 50<\/td>\n50<\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n

SE-buffers activity and inactivation temperature (20 minutes)<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Enzyme<\/span><\/b><\/td>\nRecognition sequence<\/span><\/b><\/td>\nOptimal buffer<\/span><\/b><\/td>\nBSA<\/span><\/b><\/td>\n% Activity<\/span><\/b><\/td>\nOptimal temperature, \u00b0\u0421<\/span><\/b><\/td>\nInactivation, \u00b0\u0421<\/span><\/b><\/td>\n<\/tr>\n
B<\/b><\/span><\/td>\nG<\/span><\/b><\/td>\nO<\/span><\/b><\/td>\nW<\/span><\/b><\/td>\nY<\/span><\/b><\/td>\nRose<\/span><\/b><\/td>\n<\/tr>\n
Aat II<\/td>\n
GACGT\u2191<\/b>C<\/span>
\nC\u2193<\/b>TGCAG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Abs I<\/td>\n
CC\u2191<\/b>TCGAGG<\/span>
\nGGAGCT\u2193<\/b>CC<\/span><\/center><\/td>\n
*<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Acc16 I<\/td>\n
TGC\u2191<\/b>GCA<\/span>
\nACG\u2193<\/b>CGT<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n70<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Acc36 I<\/td>\n
ACCTGC(N)4<\/sub>\u2191<\/b><\/span>
\nTGGACG(N)8<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Acc65 I<\/td>\n
G\u2191<\/b>GTACC<\/span>
\nCCATG\u2193<\/b>G<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AccB1 I<\/td>\n
G\u2191<\/b>GYRCC<\/span>
\nCCRYG\u2193<\/b>G<\/span><\/center><\/td>\n
K<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n30<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AccB7 I<\/td>\n
CCANNNN\u2191<\/b>NTGG<\/span>
\nGGTN\u2193<\/b>NNNNACC<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AccBS I<\/td>\n
GAG\u2191<\/b>CGG<\/span>
\nCTC\u2193<\/b>GCC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Acl I<\/td>\n
AA\u2191<\/b>CGTT<\/span>
\nTTGC\u2193<\/b>AA<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n80<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AclW I<\/td>\n
GGATC(N)4<\/sub>\u2191<\/b><\/span>
\nCCTAG(N)5<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n30<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Aco I<\/td>\n
Y\u2191<\/b>GGCCR<\/span>
\nRCCGG\u2193<\/b>Y<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Acs I<\/td>\n
R\u2191<\/b>AATTY<\/span>
\nYTTAA\u2193<\/b>R<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n
Acu I<\/td>\n
CTGAAG(N)16<\/sub>\u2191<\/b><\/span>
\nGACTTC(N)14<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y+ SAM<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Afe I<\/td>\n
AGC\u2191<\/b>GCT<\/span>
\nTCG\u2193<\/b>CGA<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Ags I<\/td>\n
TTS\u2191<\/b>AA<\/span>
\nAA\u2193<\/b>STT<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Ahl I<\/td>\n
A\u2191<\/b>CTAGT<\/span>
\nTGATC\u2193<\/b>A<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\nN<\/td>\n<\/tr>\n
Ajn I<\/td>\n
\u2191<\/b>CCWGG<\/span>
\nGGWCC\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n
Alu I<\/td>\n
AG\u2191<\/b>CT<\/span>
\nTC\u2193<\/b>GA<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AluB I<\/td>\n
AG\u2191<\/b>CT<\/span>
\nTC\u2193<\/b>GA<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n60<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Ama87 I<\/td>\n
C\u2191<\/b>YCGRG<\/span>
\nGRGCY\u2193<\/b>C<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n0 – 10<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Apa I<\/td>\n
GGGCC\u2191<\/b>C<\/span>
\nC\u2193<\/b>CCGGG<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Ars I<\/td>\n
\u2191<\/b>(N)8<\/sub>GACNNNNNNTTYG(N)11<\/sub>\u2191<\/b><\/span>
\n\u2193<\/b>(N)13<\/sub>CTGNNNNNNAARC(N)6<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n20<\/span><\/td>\n30<\/td>\n65<\/td>\n<\/tr>\n
AsiG I<\/td>\n
A\u2191<\/b>CCGGT<\/span>
\nTGGCC\u2193<\/b>A<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n75<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AspA2 I<\/td>\n
C\u2191<\/b>CTAGG<\/span>
\nGGATC\u2193<\/b>C<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
AspLE I<\/td>\n
GCG\u2191<\/b>C<\/span>
\nC\u2193<\/b>GCG<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\nN<\/td>\n<\/tr>\n
AspS9 I<\/td>\n
G\u2191<\/b>GNCC<\/span>
\nCCNG\u2193<\/b>G<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n75<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AsuC2 I<\/td>\n
CC\u2191<\/b>SGG<\/span>
\nGGS\u2193<\/b>CC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n40<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AsuHP I<\/td>\n
GGTGA(N)8<\/sub>\u2191<\/b><\/span>
\nCCACT(N)7<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
AsuNH I<\/td>\n
G\u2191<\/b>CTAGC<\/span>
\nCGATC\u2193<\/b>G<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BamH I<\/td>\n
G\u2191<\/b>GATCC<\/span>
\nCCTAG\u2193<\/b>G<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bar I<\/td>\n
\u2191<\/b>(N)7<\/sub>GAAGNNNNNNTAC(N)12<\/sub>\u2191<\/b><\/span>
\n\u2193<\/b>(N)12<\/sub>CTTCNNNNNNATG(N)7<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
*<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n40<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bbv12 I<\/td>\n
GWGCW\u2191<\/b>C<\/span>
\nC\u2193<\/b>WCGWG<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n60<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Bgl I<\/td>\n
GCCNNNN\u2191<\/b>NGGC<\/span>
\nCGGN\u2193<\/b>NNNNCCG<\/span><\/center><\/td>\n
2W<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bgl II<\/td>\n
A\u2191<\/b>GATCT<\/span>
\nTCTAG\u2193<\/b>A<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Bme18 I<\/td>\n
G\u2191<\/b>GWCC<\/span>
\nCCWG\u2193<\/b>G<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n80<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bmt I<\/td>\n
GCTAG\u2191<\/b>C<\/span>
\nC\u2193<\/b>GATCG<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bmu I<\/td>\n
ACTGGG(N)5<\/sub>\u2191<\/b><\/span>
\nTGACCC(N)4<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BpmI<\/td>\n
CTGGAG(N)16<\/sub>\u2191<\/b><\/span>
\nGACCTC(N)14<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bpu10I<\/td>\n
CC\u2191<\/b>TNAGC<\/span>
\nGGANT\u2193<\/b>CG<\/span><\/center><\/td>\n
K<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Bpu14 I<\/td>\n
TT\u2191<\/b>CGAA<\/span>
\nAAGC\u2193<\/b>TT<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bsa29 I<\/td>\n
AT\u2191<\/b>CGAT<\/span>
\nTAGC\u2193<\/b>TA<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Bsc4 I<\/td>\n
CCNNNNN\u2191<\/b>NNGG<\/span>
\nGGNN\u2193<\/b>NNNNNCC<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n90<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
Bse1 I<\/td>\n
ACTGGN\u2191<\/b><\/span>
\nTGAC\u2193<\/b>CN<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
Bse118 I<\/td>\n
R\u2191<\/b>CCGGY<\/span>
\nYGGCC\u2193<\/b>R<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
Bse21 I<\/td>\n
CC\u2191<\/b>TNAGG<\/span>
\nGGANT\u2193<\/b>CC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n40<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Bse3D I<\/td>\n
GCAATGNN\u2191<\/b><\/span>
\nCGTTAC\u2193<\/b>NN<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
Bse8 I<\/td>\n
GATNN\u2191<\/b>NNATC<\/span>
\nCTANN\u2193<\/b>NNTAG<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
BseP I<\/td>\n
G\u2191<\/b>CGCGC<\/span>
\nCGCGC\u2193<\/b>G<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50<\/td>\n65<\/td>\n<\/tr>\n
BseX3 I<\/td>\n
C\u2191<\/b>GGCCG<\/span>
\nGCCGG\u2193<\/b>C<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25<\/span><\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n
BslF I<\/td>\n
GGGAC(N)10<\/sub>\u2191<\/b><\/span>
\nCCCTG(N)14<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Bso31 I<\/td>\n
GGTCTC(N)1<\/sub>\u2191<\/b><\/span>
\nCCAGAG(N)5<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
O<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n40<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
Bsp13 I<\/td>\n
T\u2191<\/b>CCGGA<\/span>
\nAGGCC\u2193<\/b>T<\/span><\/center><\/td>\n
2K<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n5<\/span><\/td>\n50<\/td>\n65<\/td>\n<\/tr>\n
Bsp1720 I<\/td>\n
GC\u2191<\/b>TNAGC<\/span>
\nCGANT\u2193<\/b>CG<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Bsp19 I<\/td>\n
C\u2191<\/b>CATGG<\/span>
\nGGTAC\u2193<\/b>C<\/span><\/center><\/td>\n
2W<\/td>\n+<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n5<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BspAC I<\/td>\n
C\u2191<\/b>CGC<\/span>
\nGGC\u2193<\/b>G<\/span><\/center><\/td>\n
O<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BspFN I<\/td>\n
CG\u2191<\/b>CG<\/span>
\nGC\u2193<\/b>GC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BssEC I<\/td>\n
C\u2191<\/b>CNNGG<\/span>
\nGGNNC\u2193<\/b>C<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
BssNA I<\/td>\n
GTA\u2191<\/b>TAC<\/span>
\nCAT\u2193<\/b>ATG<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\nN<\/td>\n<\/tr>\n
BssT1 I<\/td>\n
C\u2191<\/b>CWWGG<\/span>
\nGGWWC\u2193<\/b>C<\/span><\/center><\/td>\n
2K<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
Bst2B I<\/td>\n
C\u2191<\/b>TCGTG<\/span>
\nGAGCA\u2193<\/b>C<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
Bst2U I<\/td>\n
CC\u2191<\/b>WGG<\/span>
\nGGW\u2193<\/b>CC<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n50<\/span><\/td>\n60<\/td>\nN<\/td>\n<\/tr>\n
Bst4C I<\/td>\n
ACN\u2191<\/b>GT<\/span>
\nTG\u2193<\/b>NCA<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n65<\/td>\nN<\/td>\n<\/tr>\n
Bst6 I<\/td>\n
CTCTTC(N)1<\/sub>\u2191<\/b><\/span>
\nGAGAAG(N)4<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
BstAC I<\/td>\n
GR\u2191<\/b>CGYC<\/span>
\nCYGC\u2193<\/b>RG<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
BstAF I<\/td>\n
C\u2191<\/b>TTAAG<\/span>
\nGAATT\u2193<\/b>C<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
BstAP I<\/td>\n
GCANNNN\u2191<\/b>NTGC<\/span>
\nCGTN\u2193<\/b>NNNNACG<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
BstAU I<\/td>\n
T\u2191<\/b>GTACA<\/span>
\nACATG\u2193<\/b>T<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
BstBA I<\/td>\n
YAC\u2191<\/b>GTR<\/span>
\nRTG\u2193<\/b>CAY<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
BstC8I<\/td>\n
GCN\u2191<\/b>NGC<\/span>
\nCGN\u2193<\/b>NCG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
BstDE I<\/td>\n
C\u2191<\/b>TNAG<\/span>
\nGANT\u2193<\/b>C<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
BstDS I<\/td>\n
C\u2191<\/b>CRYGG<\/span>
\nGGYRC\u2193<\/b>C<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
BstEN I<\/td>\n
CCTNN\u2191<\/b>NNNAGG<\/span>
\nGGANNN\u2193<\/b>NNTCC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
BstF5 I<\/td>\n
GGATGNN\u2191<\/b><\/span>
\nCCTAC\u2193<\/b>NN<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
BstFN I<\/td>\n
CG\u2191<\/b>CG<\/span>
\nGC\u2193<\/b>GC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
BstH2 I<\/td>\n
RGCGC\u2191<\/b>Y<\/span>
\nY\u2193<\/b>CGCGR<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
BstHH I<\/td>\n
GCG\u2191<\/b>C<\/span>
\nC\u2193<\/b>GCG<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n50<\/td>\nN<\/td>\n<\/tr>\n
BstKTI<\/td>\n
GAT\u2191<\/b>C<\/span>
\nC\u2193<\/b>TAG<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BstMA I<\/td>\n
GTCTC(N)1<\/sub>\u2191<\/b><\/span>
\nCAGAG(N)5<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n
BstMB I<\/td>\n
\u2191<\/b>GATC<\/span>
\nCTAG\u2193<\/b><\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
BstMC I<\/td>\n
CGRY\u2191<\/b>CG<\/span>
\nGC\u2193<\/b>YRGC<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n
BstMW I<\/td>\n
GCNNNNN\u2191<\/b>NNGC<\/span>
\nCGNN\u2193<\/b>NNNNNCG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n40<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
BstNS I<\/td>\n
RCATG\u2191<\/b>Y<\/span>
\nY\u2193<\/b>GTACR<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BstPA I<\/td>\n
GACNN\u2191<\/b>NNGTC<\/span>
\nCTGNN\u2193<\/b>NNCAG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\nN<\/td>\n<\/tr>\n
BstSCI<\/td>\n
\u2191<\/b>CCNGG<\/span>
\nGGNCC\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n60<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
BstSF I<\/td>\n
C\u2191<\/b>TRYAG<\/span>
\nGAYRT\u2193<\/b>C<\/span><\/center><\/td>\n
O<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\nN<\/td>\n<\/tr>\n
BstSL I<\/td>\n
GKGCM\u2191<\/b>C<\/span>
\nC\u2193<\/b>MCGKG<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n
BstSN I<\/td>\n
TAC\u2191<\/b>GTA<\/span>
\nATG\u2193<\/b>CAT<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
BstV1I<\/td>\n
GCAGC(N)8<\/sub>\u2191<\/b><\/span>
\nCGTCG(N)12<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
BstV2 I<\/td>\n
GAAGAC(N)2<\/sub>\u2191<\/b><\/span>
\nCTTCTG(N)6<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n70<\/span><\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n
BstX I<\/td>\n
CCANNNNN\u2191<\/b>NTGG<\/span>
\nGGTN\u2193<\/b>NNNNNACC<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BstX2 I Temporarily unavailable<\/span><\/td>\n
R\u2191<\/b>GATCY<\/span>
\nYCTAG\u2193<\/b>R<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
Bsu I<\/td>\n
GTATCC(N)6<\/sub>\u2191<\/b><\/span>
\nCATAGG(N)5<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n10<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
BsuR I<\/td>\n
GG\u2191<\/b>CC<\/span>
\nCC\u2193<\/b>GG<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Btr I<\/td>\n
CAC\u2191<\/b>GTC<\/span>
\nGTG\u2193<\/b>CAG<\/span><\/center><\/td>\n
O<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n60<\/td>\n80<\/td>\n<\/tr>\n
Cci I<\/td>\n
T\u2191<\/b>CATGA<\/span>
\nAGTAC\u2193<\/b>T<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
CciN I<\/td>\n
GC\u2191<\/b>GGCCGC<\/span>
\nCGCCGG\u2193<\/b>CG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Dra I<\/td>\n
TTT\u2191<\/b>AAA<\/span>
\nAAA\u2193<\/b>TTT<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
DraIII<\/td>\n
CACNNN\u2191<\/b>GTG<\/span>
\nGTG\u2193<\/b>NNNCAC<\/span><\/center><\/td>\n
2K<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Dri I<\/td>\n
GACNNN\u2191<\/b>NNGTC<\/span>
\nCTGNN\u2193<\/b>NNNCAG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n40<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
DseD I<\/td>\n
GACNNNN\u2191<\/b>NNGTC<\/span>
\nCTGNN\u2193<\/b>NNNNCAG<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n30<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
EcoICR I<\/td>\n
GAG\u2191<\/b>CTC<\/span>
\nCTC\u2193<\/b>GAG<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n75 – 100<\/span><\/td>\n5<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
EcoR I<\/td>\n
G\u2191<\/b>AATTC<\/span>
\nCTTAA\u2193<\/b>G<\/span><\/center><\/td>\n
*<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
EcoR V<\/td>\n
GAT\u2191<\/b>ATC<\/span>
\nCTA\u2193<\/b>TAG<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n0 – 10<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Ege I<\/td>\n
GGC\u2191<\/b>GCC<\/span>
\nCCG\u2193<\/b>CGG<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Erh I<\/td>\n
C\u2191<\/b>CWWGG<\/span>
\nGGWWC\u2193<\/b>C<\/span><\/center><\/td>\n
2W<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
FaeI<\/td>\n
CATG\u2191<\/b><\/span>
\n\u2193<\/b>GTAC<\/span><\/center><\/td>\n
*<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
FaiI<\/td>\n
YA\u2191<\/b>TR<\/span>
\nRT\u2193<\/b>AY<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n
FalI<\/td>\n
\u2191<\/b>(N)8<\/sub>AAGNNNNNCTT(N)13<\/sub>\u2191<\/b><\/span>
\n\u2193<\/b>(N)13<\/sub>TTCNNNNNGAA(N)8<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
W+ SAM<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n70<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
FatI<\/td>\n
\u2191<\/b>CATG<\/span>
\nGTAC\u2193<\/b><\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n
Fau I<\/td>\n
CCCGC(N)4<\/sub>\u2191<\/b><\/span>
\nGGGCG(N)6<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n50 – 75<\/span><\/td>\n50<\/span><\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n
FauND I<\/td>\n
CA\u2191<\/b>TATG<\/span>
\nGTAT\u2193<\/b>AC<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Fbl I<\/td>\n
GT\u2191<\/b>MKAC<\/span>
\nCAKM\u2193<\/b>TG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n0 – 10<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
Fok I<\/td>\n
GGATG(N)9<\/sub>\u2191<\/b><\/span>
\nCCTAC(N)13<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
FriO I<\/td>\n
GRGCY\u2191<\/b>C<\/span>
\nC\u2193<\/b>YCGRG<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Fsp4H I<\/td>\n
GC\u2191<\/b>NGC<\/span>
\nCGN\u2193<\/b>CG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Gsa I<\/td>\n
CCCAG\u2191<\/b>C<\/span>
\nG\u2193<\/b>GGTCG<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n70<\/td>\nN<\/td>\n<\/tr>\n
Hae III<\/td>\n
GG\u2191<\/b>CC<\/span>
\nCC\u2193<\/b>GG<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Hga I<\/td>\n
GACGC(N)5<\/sub>\u2191<\/b><\/span>
\nCTGCG(N)10<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Hind II<\/td>\n
GTY\u2191<\/b>RAC<\/span>
\nCAR\u2193<\/b>YTG<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Hind III<\/td>\n
A\u2191<\/b>AGCTT<\/span>
\nTTCGA\u2193<\/b>A<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Hinf I<\/td>\n
G\u2191<\/b>ANTC<\/span>
\nCTNA\u2193<\/b>G<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Hpa I<\/td>\n
GTT\u2191<\/b>AAC<\/span>
\nCAA\u2193<\/b>TTG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Hpa II<\/td>\n
C\u2191<\/b>CGG<\/span>
\nGGC\u2193<\/b>C<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
HpySE526 I<\/td>\n
A\u2191<\/b>CGT<\/span>
\nTGC\u2193<\/b>A<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
HspA I<\/td>\n
G\u2191<\/b>CGC<\/span>
\nCGC\u2193<\/b>G<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Kpn I<\/td>\n
GGTAC\u2191<\/b>C<\/span>
\nC\u2193<\/b>CATGG<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Ksp22 I<\/td>\n
T\u2191<\/b>GATCA<\/span>
\nACTAG\u2193<\/b>T<\/span><\/center><\/td>\n
2K<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Kzo9 I<\/td>\n
\u2191<\/b>GATC<\/span>
\nCTAG\u2193<\/b><\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Lmn I<\/td>\n
GCTCCN\u2191<\/b><\/span>
\nCGAG\u2193<\/b>GN<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n80<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Mab I<\/td>\n
A\u2191<\/b>CCWGGT<\/span>
\nTGGWCC\u2193<\/b>A<\/span><\/center><\/td>\n
W<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Mbo II<\/td>\n
GAAGA(N)8<\/sub>\u2191<\/b><\/span>
\nCTTCT(N)7<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Mfe I<\/td>\n
C\u2191<\/b>AATTG<\/span>
\nGTTAA\u2193<\/b>C<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\nN<\/td>\n<\/tr>\n
Mhl I<\/td>\n
GDGCH\u2191<\/b>C<\/span>
\nC\u2193<\/b>HCGDG<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Mlu I<\/td>\n
A\u2191<\/b>CGCGT<\/span>
\nTGCGC\u2193<\/b>A<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Mly113 I<\/td>\n
GG\u2191<\/b>CGCC<\/span>
\nCCGC\u2193<\/b>GG<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Mnl I<\/td>\n
CCTC(N)7<\/sub>\u2191<\/b><\/span>
\nGGAG(N)6<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Mox20 I<\/td>\n
TGG\u2191<\/b>CCA<\/span>
\nACC\u2193<\/b>GGT<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n75<\/span><\/td>\n37<\/td>\nN<\/td>\n<\/tr>\n
MroN I<\/td>\n
G\u2191<\/b>CCGGC<\/span>
\nCGGCC\u2193<\/b>G<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n5<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
MroX I<\/td>\n
GAANN\u2191<\/b>NNTTC<\/span>
\nCTTNN\u2193<\/b>NNAAG<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Msp I<\/td>\n
C\u2191<\/b>CGG<\/span>
\nGGC\u2193<\/b>C<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
MspA1 I<\/td>\n
CMG\u2191<\/b>CKG<\/span>
\nGKC\u2193<\/b>GMC<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
MspR9 I<\/td>\n
CC\u2191<\/b>NGG<\/span>
\nGGN\u2193<\/b>CC<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Nru I<\/td>\n
TCG\u2191<\/b>CGA<\/span>
\nAGC\u2193<\/b>GCT<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
PalA I<\/td>\n
GG\u2191<\/b>CGCGCC<\/span>
\nCCGCGC\u2193<\/b>GG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n40<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Pec I<\/td>\n
TTA\u2191TAA<\/center>
AAT\u2193ATT<\/center><\/td>\n
Y<\/td>\n–<\/td>\n50-75<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Pce I<\/td>\n
AGG\u2191<\/b>CCT<\/span>
\nTCC\u2193<\/b>GGA<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n50<\/td>\n80<\/td>\n<\/tr>\n
Pci I<\/td>\n
A\u2191<\/b>CATGT<\/span>
\nTGTAC\u2193<\/b>A<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
PciS I<\/td>\n
GCTCTTCN\u2191<\/b><\/span>
\nCGAGAAG(N)4<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n75 – 100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Pct I<\/td>\n
GAATGCN\u2191<\/b><\/span>
\nCTTAC\u2193<\/b>GN<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Ple19 I<\/td>\n
CGAT\u2191<\/b>CG<\/span>
\nGC\u2193<\/b>TAGC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Pps I<\/td>\n
GAGTC(N)4<\/sub>\u2191<\/b><\/span>
\nCTCAG(N)5<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Pse31 I<\/td>\n
GGTCTC(N)1<\/sub>\u2191<\/b><\/span>
\nCCAGAG(N)5<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n25-50<\/span><\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Psi I<\/td>\n
TTA\u2191<\/b>TAA<\/span>
\nAAT\u2193<\/b>ATT<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n40<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Psp124B I<\/td>\n
GAGCT\u2191<\/b>C<\/span>
\nC\u2193<\/b>TCGAG<\/span><\/center><\/td>\n
G<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n75 – 100<\/span><\/td>\n30<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Psp6 I<\/td>\n
\u2191<\/b>CCWGG<\/span>
\nGGWCC\u2193<\/b><\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n10<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
PspC I<\/td>\n
CAC\u2191<\/b>GTG<\/span>
\nGTG\u2193<\/b>CAC<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n50 – 75<\/span><\/td>\n5<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
PspE I<\/td>\n
G\u2191<\/b>GTNACC<\/span>
\nCCANTG\u2193<\/b>G<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
PspL I<\/td>\n
C\u2191<\/b>GTACG<\/span>
\nGCATG\u2193<\/b>C<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
PspN4 I<\/td>\n
GGN\u2191<\/b>NCC<\/span>
\nCCN\u2193<\/b>NGG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
PspOM I<\/td>\n
G\u2191<\/b>GGCCC<\/span>
\nCCCGG\u2193<\/b>G<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
PspPP I<\/td>\n
RG\u2191<\/b>GWCCY<\/span>
\nYCCWG\u2193<\/b>GR<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
PspX I<\/td>\n
VC\u2191<\/b>TCGAGB<\/span>
\nBGAGCT\u2193<\/b>CV<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Psr I<\/td>\n
\u2191<\/b>(N)7<\/sub>GAACNNNNNNTAC(N)12<\/sub>\u2191<\/b><\/span>
\n\u2193<\/b>(N)12<\/sub>CTTGNNNNNNATG(N)7<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n30<\/span><\/td>\n30<\/td>\n65<\/td>\n<\/tr>\n
Pst I<\/td>\n
CTGCA\u2191<\/b>G<\/span>
\nG\u2193<\/b>ACGTC<\/span><\/center><\/td>\n
O<\/td>\n+<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
PstN I<\/td>\n
CAGNNN\u2191<\/b>CTG<\/span>
\nGTC\u2193<\/b>NNNGAC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Pvu II<\/td>\n
CAG\u2191<\/b>CTG<\/span>
\nGTC\u2193<\/b>GAC<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Rga I<\/td>\n
GCGAT\u2191<\/b>CGC<\/span>
\nCGC\u2193<\/b>TAGCG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
Rig I<\/td>\n
GGCCGG\u2191<\/b>CC<\/span>
\nCC\u2193<\/b>GGCCGG<\/span><\/center><\/td>\n
*<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n10<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Rsa I<\/td>\n
GT\u2191<\/b>AC<\/span>
\nCA\u2193<\/b>TG<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\nN<\/td>\n<\/tr>\n
RsaN I<\/td>\n
G\u2191<\/b>TAC<\/span>
\nCAT\u2193<\/b>G<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Rsr2 I<\/td>\n
CG\u2191<\/b>GWCCG<\/span>
\nGCCWG\u2193<\/b>GC<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Sal I<\/td>\n
G\u2191<\/b>TCGAC<\/span>
\nCAGCT\u2193<\/b>G<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n0 – 10<\/span><\/td>\n5<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Sbf I<\/td>\n
CCTGCA\u2191<\/b>GG<\/span>
\nGG\u2193<\/b>ACGTCC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Set I<\/td>\n
ASST\u2191<\/b><\/span>
\n\u2193<\/b>TSSA<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n55<\/td>\n80<\/td>\n<\/tr>\n
SfaN I<\/td>\n
GCATC(N)5<\/sub>\u2191<\/b><\/span>
\nCGTAG(N)9<\/sub>\u2193<\/b><\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n0 – 10<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Sfi I<\/td>\n
GGCCNNNN\u2191<\/b>NGGCC<\/span>
\nCCGGN\u2193<\/b>NNNNCCGG<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75<\/span><\/td>\n50<\/td>\n65<\/td>\n<\/tr>\n
Sfr274 I<\/td>\n
C\u2191<\/b>TCGAG<\/span>
\nGAGCT\u2193<\/b>C<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50<\/td>\n65<\/td>\n<\/tr>\n
Sfr303 I<\/td>\n
CCGC\u2191<\/b>GG<\/span>
\nGG\u2193<\/b>CGCC<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Sma I<\/td>\n
CCC\u2191<\/b>GGG<\/span>
\nGGG\u2193<\/b>CCC<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n25<\/td>\n65<\/td>\n<\/tr>\n
Smi I<\/td>\n
ATTT\u2191<\/b>AAAT<\/span>
\nTAAA\u2193<\/b>TTTA<\/span><\/center><\/td>\n
O<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n10<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
SmiM I<\/td>\n
CAYNN\u2191<\/b>NNRTG<\/span>
\nGTRNN\u2193<\/b>NNYAC<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n10 – 25<\/span><\/td>\n60<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Sph I<\/td>\n
GCATG\u2191<\/b>C<\/span>
\nC\u2193<\/b>GTACG<\/span><\/center><\/td>\n
G<\/td>\n+<\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Sse9 I<\/td>\n
\u2191<\/b>AATT<\/span>
\nTTAA\u2193<\/b><\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75<\/span><\/td>\n55<\/td>\n65<\/td>\n<\/tr>\n
Ssp I<\/td>\n
AAT\u2191<\/b>ATT<\/span>
\nTTA\u2193<\/b>TAA<\/span><\/center><\/td>\n
K<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Taq I<\/td>\n
T\u2191<\/b>CGA<\/span>
\nAGC\u2193<\/b>T<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
Tru9 I<\/td>\n
T\u2191<\/b>TAA<\/span>
\nAAT\u2193<\/b>T<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
TseF I<\/td>\n
\u2191<\/b>GTSAC<\/span>
\nCASTG\u2193<\/b><\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n25 – 50<\/span><\/td>\n50 – 75<\/span><\/td>\n50<\/span><\/td>\n65<\/td>\nN<\/td>\n<\/tr>\n
Tth111 I<\/td>\n
GACN\u2191<\/b>NNGTC<\/span>
\nCTGNN\u2193<\/b>NCAG<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n10 – 25<\/span><\/td>\n10 – 25<\/span><\/td>\n100<\/span><\/td>\n100<\/span><\/td>\n65<\/td>\n80<\/td>\n<\/tr>\n
Vne I<\/td>\n
G\u2191<\/b>TGCAC<\/span>
\nCACGT\u2193<\/b>G<\/span><\/center><\/td>\n
O<\/td>\n–<\/td>\n10 – 25<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Vsp I<\/td>\n
AT\u2191<\/b>TAAT<\/span>
\nTAAT\u2193<\/b>TA<\/span><\/center><\/td>\n
W<\/td>\n–<\/td>\n0 – 10<\/span><\/td>\n10 – 25<\/span><\/td>\n50 – 75<\/span><\/td>\n100<\/span><\/td>\n25 – 50<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Xba I<\/td>\n
T\u2191<\/b>CTAGA<\/span>
\nAGATC\u2193<\/b>T<\/span><\/center><\/td>\n
O<\/td>\n+<\/td>\n75 – 100<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n75 – 100<\/span><\/td>\n25<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Xma I<\/td>\n
C\u2191<\/b>CCGGG<\/span>
\nGGGCC\u2193<\/b>C<\/span><\/center><\/td>\n
Y<\/td>\n–<\/td>\n75 – 100<\/span><\/td>\n50 – 75<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
ZraI<\/td>\n
GAC\u2191<\/b>GTC<\/span>
\nCTG\u2193<\/b>CAG<\/span><\/center><\/td>\n
B<\/td>\n–<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n75 – 100<\/span><\/td>\n100<\/span><\/td>\n37<\/td>\n80<\/td>\n<\/tr>\n
Zrm I<\/td>\n
AGT\u2191<\/b>ACT<\/span>
\nTCA\u2193<\/b>TGA<\/span><\/center><\/td>\n
Y<\/td>\n+<\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n0 – 10<\/span><\/td>\n0 – 10<\/span><\/td>\n100<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n
Zsp2 I<\/td>\n
ATGCA\u2191<\/b>T<\/span>
\nT\u2193<\/b>ACGTA<\/span><\/center><\/td>\n
B<\/td>\n+<\/td>\n100<\/span><\/td>\n50 – 75<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n25 – 50<\/span><\/td>\n50<\/span><\/td>\n37<\/td>\n65<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

SE-buffers activity and inactivation temperature (20 minutes) Enzyme Recognition sequence Optimal buffer BSA % Activity Optimal temperature, \u00b0\u0421 Inactivation, \u00b0\u0421 B G O W Y Rose Aat II GACGT\u2191C C\u2193TGCAG Y – 10 – 25 25 – 50 10 – 25 25 – 50 100 50 37 65 Abs I CC\u2191TCGAGG GGAGCT\u2193CC * – 75 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-3026","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages\/3026","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/comments?post=3026"}],"version-history":[{"count":2,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages\/3026\/revisions"}],"predecessor-version":[{"id":5170,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages\/3026\/revisions\/5170"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=3026"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}