{"id":3077,"date":"2022-05-19T15:07:58","date_gmt":"2022-05-19T08:07:58","guid":{"rendered":"http:\/\/sibenzyme.com\/?page_id=3077"},"modified":"2026-04-28T11:38:47","modified_gmt":"2026-04-28T04:38:47","slug":"protokol-turbo","status":"publish","type":"page","link":"https:\/\/sibenzyme.com\/en\/protokol-turbo\/","title":{"rendered":"Turbo digestion protocol"},"content":{"rendered":"

<\/p>\n

\n
\n

Fast DNA Digestion with Turbo Restriction Endonucleases<\/h1>\n

Standard Protocol and Conditions for Turbo<\/strong> Enzymes.<\/p>\n<\/header>\n

\n

Properties of Turbo Restriction Endonucleases<\/h2>\n

Turbo enzymes have been tested for their ability to digest DNA within a short incubation time.
\nDuring testing, 1 \u03bcl of a Turbo enzyme was added to a reaction mixture containing 1 \u03bcg of DNA (phage or plasmid) and 1\u00d7 universal ROSE buffer (for some enzymes, their own optimal buffer was used). After incubation for 10 or 15 minutes, complete digestion of the DNA substrate was observed.<\/p>\n<\/section>\n

\n

Standard Turbo Digestion Reaction Protocol<\/h2>\n
\n
Reaction volume: 20 \u03bcl<\/strong><\/div>\n<\/div>\n
\n
\n

10\u00d7 SE Buffer ROSE (or optimal buffer)<\/span><\/p>\n

2 \u03bcl<\/span><\/p>\n<\/div>\n

\n

DNA preparation<\/span><\/p>\n

0,2\u20131 \u03bcg<\/span><\/p>\n<\/div>\n

\n

BSA (10 mg\/ml) (optional)*<\/span><\/p>\n

0,2 \u03bcl<\/span><\/p>\n<\/div>\n

\n

Sterile water<\/span><\/p>\n

\u0434\u043e 20 \u03bcl<\/span><\/p>\n<\/div>\n<\/div>\n

+<\/strong> 1 \u03bcl Turbo restriction endonuclease
\n\/ or 1 \u03bcl of each of two Turbo enzymes for double digestion<\/span>.<\/p>\n

    \n
  1. Mix gently.<\/li>\n
  2. Incubate at the optimal temperature for 10<\/strong> or 15<\/strong> minutes..<\/li>\n<\/ol>\n

    * Add to the reaction if BSA is required for maximal enzyme activity.<\/p>\n<\/section>\n

    \n

    Notes<\/h3>\n

    These enzymes should be used only in undiluted form.<\/p>\n

    For fast digestion, highly purified DNA preparations should be used, especially PCR fragments.
    \nComplete digestion of some PCR products and supercoiled plasmid DNA may require longer than 10\u201315 minutes incubation due to their lower susceptibility to cleavage.<\/p>\n<\/section>\n<\/div>\n

    <\/p>","protected":false},"excerpt":{"rendered":"

    Fast DNA Digestion with Turbo Restriction Endonucleases Standard Protocol and Conditions for Turbo Enzymes. Properties of Turbo Restriction Endonucleases Turbo enzymes have been tested for their ability to digest DNA within a short incubation time. During testing, 1 \u03bcl of a Turbo enzyme was added to a reaction mixture containing 1 \u03bcg of DNA (phage […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-3077","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages\/3077","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/comments?post=3077"}],"version-history":[{"count":2,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages\/3077\/revisions"}],"predecessor-version":[{"id":4978,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages\/3077\/revisions\/4978"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=3077"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}