{"id":747,"date":"2022-03-22T10:10:08","date_gmt":"2022-03-22T03:10:08","guid":{"rendered":"http:\/\/sibenzyme.com\/?page_id=747"},"modified":"2025-11-26T14:15:54","modified_gmt":"2025-11-26T07:15:54","slug":"epigenetics","status":"publish","type":"page","link":"https:\/\/sibenzyme.com\/en\/epigenetics\/","title":{"rendered":"Epigenetics"},"content":{"rendered":"

[vc_row css=”.vc_custom_1652439246075{margin-top: 0px !important;margin-bottom: 0px !important;padding-top: 0px !important;padding-bottom: 0px !important;}”][vc_column][vc_column_text css=”.vc_custom_1652439062077{margin: 0px !important;padding: 0px !important;border: 0px initial !important;}”]<\/p>\n

Epigenetics<\/h1>\n

[\/vc_column_text][vc_column_text css=”.vc_custom_1652439071326{margin: 0px !important;padding: 0px !important;border: 0px initial !important;}”]SibEnzyme has recently developed full range of technologies for DNA methylation assays and epigenetic tests development, including new type of enzymes cuting only methylated DNA, NGS DNA methylation profiling for search of potentyal epigenetic biomarkers and DNA PCR methylation assays for study of DNA methylation in particular region.[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1652439276761{margin-top: 0px !important;margin-bottom: 0px !important;padding-top: 0px !important;padding-bottom: 0px !important;background-color: #e5ebff !important;}”][vc_column][vc_row_inner css=”.vc_custom_1652439099798{margin: 0px !important;padding: 0px !important;border: 0px initial !important;}”][vc_column_inner width=”1\/4″ css=”.vc_custom_1652439110725{margin: 0px !important;padding: 0px !important;border: 0px initial !important;}”][vc_column_text]Assay is based on a new type of enzymes<\/mark>, recently developed by SibEnzyme – methyl-directed DNA endonucleases or MD DNA endonucleases. MD endonucleases work like traditional restriction enzymes, but they cut only methylated DNA and may be used in epigenetic studies. More details<\/a>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/4″][vc_column_text css=”.vc_custom_1652439119612{margin: 0px !important;padding: 0px !important;border: 0px initial !important;}”]2 principaly new methods: GLAD-PCR assay and <\/mark>GlaI-PCR assay<\/mark>– simple and inexpensive method for epigenetic studies of DNA preparations including ones from clinical samples. They allow to detect even several DNA molecules with R(5mC)GY site of interest<\/mark> in a presence of excessive amount of unmethylated DNA or several DNA molecules with RCGY site of interest<\/mark> in a presence of excessive amount of methylated DNA. Just several easy steps in one tube. More details on GLAD-PCR<\/a> or GlaI-PCR<\/a>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/4″][vc_column_text css=”.vc_custom_1652439150666{margin: 0px !important;padding: 0px !important;border: 0px initial !important;}”]Genomic DNA fragmentation with GlaI<\/mark> and subsequent NGS<\/mark> allow to construct a map of R(5mC)GY sites in analyzed genomes, for example of patients and healthy donors. Comparison of these maps reveals R(5mC)GY sites – potential epigenetic markers<\/mark> that can be used for invitro<\/em> diagnostics. More details<\/a>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/4″][vc_column_text css=”.vc_custom_1652439141118{margin: 0px !important;padding: 0px !important;border: 0px initial !important;}”]Light fraction of the blood cells<\/mark> is a good source of DNA for our studies, althogh you may work with any source of DNA. It’s easy to prepare this fraction \u2013 one standard table centrifuge, no reagents except saline and minimal time of manipulation with a blood. More details<\/a>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_column_text css=”.vc_custom_1652439162872{margin: 0px !important;padding: 0px !important;border: 0px initial !important;}”]<\/p>\n

Conferences, patents and publications<\/a><\/p>\n

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New type of enzymes – MD DNA endonucleases<\/h2>\n

[\/vc_column_text][vc_row_inner][vc_column_inner width=”2\/3″][vc_column_text]Methyl-directed site-specific DNA endonucleases belong to a new type of enzymes discovered by SibEnzyme (Russia).<\/p>\n

These enzymes are very similar to restriction enzymes in biochemical properties and cleave DNA completely. A principal difference of new type of enzymes: they cleave only methylated DNA. This property makes MD endonucleases to be a good instrument for epigenetic analysis.<\/p>\n

Now\u00a0 9 different MD DNA-endonucleases are commercially available<\/a>.\u00a0Recognition sites of MD enzymes differ in DNA structure and the methylation patterns plus the enzymes may cleave the same recognition site in a different way.<\/p>\n

A special advantage of GlaI<\/a> recognition site R(5mC)GY – this site is the same as a product of DNA methylation with DNMT3, which is responsible for DNA methylation de novo<\/em>. It makes GlaI to be a very convenient tool for epigenetic diagnostics<\/p>\n

Known applications:<\/p>\n