{"id":994,"date":"2022-03-25T11:15:25","date_gmt":"2022-03-25T04:15:25","guid":{"rendered":"http:\/\/sibenzyme.com\/?page_id=994"},"modified":"2025-10-09T12:41:22","modified_gmt":"2025-10-09T05:41:22","slug":"buffers","status":"publish","type":"page","link":"https:\/\/sibenzyme.com\/en\/buffers\/","title":{"rendered":"Optimal buffers contents"},"content":{"rendered":"

[vc_row][vc_column][vc_column_text]<\/p>\n

Optimal buffers contents<\/h1>\n

[\/vc_column_text][vc_column_text]<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Buffer<\/strong><\/td>\nContent<\/strong><\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer B<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer B+<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT; 100 \u03bcg\/ml BSA .<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer G<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer G+<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT; 100 \u03bcg\/ml BSA.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer O<\/td>\n\u00a0\u00a050 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer O+<\/td>\n\u00a0\u00a050 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT; 100 \u03bcg\/ml BSA.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer W<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer W+<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT; 100 \u03bcg\/ml BSA.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer Y<\/td>\n\u00a0\u00a033 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer Y+<\/td>\n\u00a0\u00a033 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT; 100 \u03bcg\/ml BSA.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer K<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer 2K<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer AbsI<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 9.0 at 25\u00b0C); 10 mM MgCl2; 50 mM KCl; 1mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer AoxI<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 8.5 ??? 25\u00b0C); 3 mM MgCl2, 1 mM DTT<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer BisI<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 9,0 at 25\u00b0C); 10 mM MgCl2; 150 mM KCl; 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer EcoRI<\/td>\n\u00a0\u00a0100 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer FaeI<\/td>\n\u00a0\u00a033 mM Tris-acetate (pH 8.3 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer GLAD<\/td>\n\u00a0\u00a050 mM Tris-SO4, pH 9.0 at 25\u00b0C; 30 mM KCl; 10 mM [NH4]2SO4<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer MalI<\/td>\n\u00a0\u00a020 mM Tris-HCl (pH 9.0 at 25\u00b0C); 10 mM MgCl2; 150 mM NaCl; 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer N.Bst9I<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 150 mM KCl; 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer PcsI<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 8.3 at 25\u00b0C); 20mM NaCl, 3 mM MgCl2, 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer RigI<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 8.5 at 25\u00b0C); 5 mM MgCl2; 1mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer polynucleotide kinase T4<\/td>\n\u00a0\u00a050 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 5 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer DNA ligase T4<\/td>\n\u00a0\u00a050 mM Tris-HCl (pH 7.8 at 25\u00b0C); 10 mM MgCl2; 10 mM DTT; 1 mM ATP.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer Klenow fragment<\/td>\n\u00a0\u00a050 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 5 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer AS (Ammonium Sulfate)<\/td>\n\u00a0\u00a067 mM Tris-HCl (pH 8.8 at 25\u00b0C); 16.6 mM (NH4)2SO4, 0.01 % Tween-20. Supplementary material is 50 mM MgCl2.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer DNA polymerase Taq<\/td>\n\u00a0\u00a060 mM Tris-HCl (pH 8.5 at 25\u00b0C); 1.5 mM MgCl2; 25 mM KCl; 10 mM 2-mercaptoethanol; 0.1% Triton X-100.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer DNA polymerase Taq (Mg2+ free)<\/td>\n\u00a0\u00a060 mM Tris-HCl (pH 8.5 at 25\u00b0C); 25 mM KCl; 10 mM 2-mercaptoethanol; 0.1% Triton X-100.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer DNA polymerase T4<\/td>\n\u00a0\u00a067 mM Tris-HCl (pH 8.8 at 25\u00b0C); 6.7 mM MgCl2;
\n16.7 mM (NH4)2SO4, 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer RNA polymerase T7<\/td>\n\u00a0\u00a050 mM Tris-HCl (pH 7.5 at 25\u00b0C); 6 mM MgCl2; 10 mM Dithiothreitol; 2 mM spermidine.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer Reverse transcriptase M-MuLV<\/td>\n\u00a0\u00a050 mM Tris-HCl (pH 8.3 at 25\u00b0C); 3 mM MgCl2; 75 mM KCl; 10 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer Reverse transcriptase M-MuLV dilution<\/td>\n\u00a0\u00a010 mM KH2PO4(pH 7.5); 0,1 mM EDTA; 200 mM NaCl; 7 mM 2-mercaptoethanol; 50% glycerol<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer Inorganic pyrophosphatase<\/td>\n\u00a0\u00a050 mM Tris-HCl (pH 8.5 at 25\u00b0C); 1 mM MgCl2.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer Exonuclease III<\/td>\n\u00a0\u00a050 mM Tris-HCl (pH 7.6 at 25\u00b0C); 1 mM MgCl2.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer Uracil DNA Glycosylase<\/td>\n\u00a0\u00a020 mM Tris-HCl (pH 8.0 at 25\u00b0C); 1 mM EDTA; 1 mM DTT<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer 2W<\/td>\n\u00a0\u00a020 mM Tris-HCl (pH 8,5 at 25\u00b0C); 10 mM MgCl2; 200 mM NaCl; 1 mM DTT<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer 2W+<\/td>\n\u00a0\u00a020 mM Tris-HCl (pH 8,5 at 25\u00b0C); 10 mM MgCl2; 200 mM NaCl; 1 mM DTT ; 100 \u03bcg\/ml BSA<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer Endonuclease I<\/td>\n\u00a0\u00a020 mM Glycine-NaOH (pH 9.5 at 25\u00b0C); 25 mM MgCl2; 100mM NaCl; 1mM 2-mercaptoethanol<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer Alkaline phosphatase<\/td>\n\u00a0\u00a050 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer RNA ligase T4<\/td>\n\u00a0\u00a050 mM Tris-HCl (pH 7.8 at 25\u00b0C); 10 mM MgCl2; 10 mM DTT; 1 mM ATP.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer Hot Start Taq DNA polymerase<\/td>\n\u00a0\u00a067 mM Tris-HCl (pH 8.8 at 25\u00b0C); 16.6 mM (NH4)2SO4, 0.01 % Tween-20. Supplementary material is 50 mM MgCl2.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer PfuSE DNA polymerase<\/td>\n\u00a0\u00a020 mM Tris-HCl (pH 8.8 at 25\u00b0C); 10 mM KCl, 10 mM (NH4)2SO4, 2 mM MgSO4, 0.1 % Triton X-100 )+ 100 \u03bcg\/ml BSA<\/strong><\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer A (Storage and dilution)<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 7.6 at 25\u00b0C); 50 mM KCl; 0,1 mM EDTA; 200 mkg\/ml BSA; 1mM DTT; 50% glycerol.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-Loading buffer
\nfor DNA Ladders<\/td>\n		\u00a0\u00a00.01% bromophenol blue; 0.25M EDTA; 50% glycerol.<\/td>\n<\/tr>\n
\u00a0\u00a0SE-buffer TMN<\/td>\n\u00a0\u00a010 mM Tris-HCl (pH 7.9 at 25\u00b0C); 5 mM MgCl2; 25 mM NaCl.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

[\/vc_column_text][\/vc_column][\/vc_row]<\/p>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column][vc_column_text] Optimal buffers contents [\/vc_column_text][vc_column_text] Buffer Content \u00a0\u00a0SE-buffer B \u00a0\u00a010 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT. \u00a0\u00a0SE-buffer B+ \u00a0\u00a010 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 1 mM DTT; 100 \u03bcg\/ml BSA . \u00a0\u00a0SE-buffer G \u00a0\u00a010 mM Tris-HCl (pH 7.6 at 25\u00b0C); 10 mM MgCl2; 50 mM […]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-994","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages\/994","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/comments?post=994"}],"version-history":[{"count":2,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages\/994\/revisions"}],"predecessor-version":[{"id":4600,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/pages\/994\/revisions\/4600"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=994"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}