{"id":4272,"date":"2025-02-28T17:29:39","date_gmt":"2025-02-28T10:29:39","guid":{"rendered":"https:\/\/sibenzyme.com\/?p=4272"},"modified":"2025-12-29T11:30:43","modified_gmt":"2025-12-29T04:30:43","slug":"novyj-ferment-pse-31-i-istinnyj-izoshizomer-restriktazy-eco-31-i","status":"publish","type":"post","link":"https:\/\/sibenzyme.com\/en\/2025\/novyj-ferment-pse-31-i-istinnyj-izoshizomer-restriktazy-eco-31-i\/","title":{"rendered":"The new enzyme Pse31I is a true isoschizomer of the restriction endonuclease Eco31I (Bso31I)"},"content":{"rendered":"

<\/p>\n

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We have discovered a bacterial strain, Peribacillus species<\/em> 31, which is a producer of a new restriction endonuclease Pse31I.
The enzyme recognizes a six-nucleotide non-palindromic DNA sequence, 5\u2019-GGTCTC-3\u2019, and cleaves it away from the recognition site at positions
1\/5: 5\u2019-GGTCTC(N)1^ \/ 3\u2019-CCAGAG(N)5^-5\u2019. Thus, Pse31I is a true isoschizomer of the restriction enzyme Eco31I.<\/p>\n

The producer strain was identified based on morphological and biochemical characteristics, as well as the primary structure analysis of a 16S rRNA gene fragment.<\/p>\n

Pse31I exhibits maximum activity at 37\u00b0C and is inactivated by incubation at 55\u00b0C and 65\u00b0C.
The enzyme does not cleave sites with methylated cytosine in the top strand (5\u2019-GGTCT(5mC)-3\u2019) and\/or the bottom strand (3\u2019-(5mC)CAGAG).<\/p>\n

The optimal working conditions for the enzyme are SE buffer 5 (Y) (33 mM Tris-acetate (pH 7.9), 10 mM Mg-acetate, 66 mM K-acetate, 1 mM DTT) and a temperature of 37\u00b0C.<\/p>\n

The enzyme is available in both regular version and the Turbo version for rapid hydrolysis.
You can order the enzyme on our website<\/a>.<\/p>\n<\/div>\n

<\/p>","protected":false},"excerpt":{"rendered":"

We have discovered a bacterial strain, Peribacillus species 31, which produces a new restriction endonuclease named Pse31I. This enzyme recognizes the six-nucleotide non-palindromic sequence 5\u2019-GGTCTC-3\u2019 (1\/5)<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[923,84,85],"tags":[708],"class_list":["post-4272","post","type-post","status-publish","format-standard","hentry","category-english","category-katalog","category-novosti","tag-novyj-produkt"],"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts\/4272","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/comments?post=4272"}],"version-history":[{"count":2,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts\/4272\/revisions"}],"predecessor-version":[{"id":4392,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts\/4272\/revisions\/4392"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=4272"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/categories?post=4272"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/tags?post=4272"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}