{"id":4729,"date":"2025-11-21T11:33:48","date_gmt":"2025-11-21T04:33:48","guid":{"rendered":"https:\/\/sibenzyme.com\/?p=4729"},"modified":"2025-12-04T13:53:23","modified_gmt":"2025-12-04T06:53:23","slug":"novyj-ferment-bmo-i","status":"publish","type":"post","link":"https:\/\/sibenzyme.com\/en\/2025\/novyj-ferment-bmo-i\/","title":{"rendered":"New restriction endonuclease Bmo I"},"content":{"rendered":"<p>[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<p data-start=\"55\" data-end=\"501\"><strong data-start=\"55\" data-end=\"165\">We are pleased to announce the release of a new restriction endonuclease \u2014 the large-cleaving enzyme BmoI.<\/strong><br data-start=\"165\" data-end=\"168\" \/>BmoI recognizes the palindromic heptanucleotide sequence <strong data-start=\"225\" data-end=\"256\">5\u2019-GG\u2193GWCCC-3\u2019 (W = A or T)<\/strong> and cleaves DNA to produce <strong data-start=\"284\" data-end=\"312\">3-nucleotide sticky ends<\/strong>.<br data-start=\"313\" data-end=\"316\" \/>Although BmoI is an isoschizomer of the previously described enzyme SanDI, it stands out as <strong data-start=\"408\" data-end=\"501\">the first commercially available restriction endonuclease with this recognition sequence.<\/strong><\/p>\n<p data-start=\"503\" data-end=\"607\">The optimal reaction conditions for BmoI are <strong data-start=\"548\" data-end=\"563\">SE-buffer W<\/strong> and an incubation temperature of <strong data-start=\"597\" data-end=\"606\">37 \u00b0C<\/strong>.<\/p>\n<p data-start=\"609\" data-end=\"805\"><a href=\"https:\/\/sibenzyme.com\/en\/product\/bmo-i\/\"><strong data-start=\"609\" data-end=\"644\">BmoI is now available for order<\/strong><\/a> and can be efficiently applied in <strong data-start=\"679\" data-end=\"735\">molecular cloning, genetic engineering, DNA analysis<\/strong>, and other workflows requiring highly specific restriction digestion.<\/p>\n<p>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;4762&#8243; img_size=&#8221;large&#8221;][\/vc_column][\/vc_row]<\/p>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text] We are pleased to announce the release of a new restriction endonuclease \u2014 the large-cleaving enzyme BmoI.BmoI recognizes the palindromic heptanucleotide sequence 5\u2019-GG\u2193GWCCC-3\u2019 (W = A or T) and cleaves DNA to produce 3-nucleotide sticky ends.Although BmoI is an isoschizomer of the previously described enzyme SanDI, it stands out as the first commercially [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[923,84],"tags":[974,708],"class_list":["post-4729","post","type-post","status-publish","format-standard","hentry","category-english","category-katalog","tag-bmoi","tag-novyj-produkt"],"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts\/4729","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/comments?post=4729"}],"version-history":[{"count":2,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts\/4729\/revisions"}],"predecessor-version":[{"id":4764,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts\/4729\/revisions\/4764"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=4729"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/categories?post=4729"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/tags?post=4729"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}