{"id":4798,"date":"2025-12-26T11:42:28","date_gmt":"2025-12-26T04:42:28","guid":{"rendered":"https:\/\/sibenzyme.com\/?p=4798"},"modified":"2025-12-29T11:27:05","modified_gmt":"2025-12-29T04:27:05","slug":"english-emii-restriction-endonuclease-is-now-commercially-available","status":"publish","type":"post","link":"https:\/\/sibenzyme.com\/en\/2025\/english-emii-restriction-endonuclease-is-now-commercially-available\/","title":{"rendered":"EmiI Restriction Endonuclease Is Now Commercially Available"},"content":{"rendered":"<p>[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<p data-start=\"202\" data-end=\"384\"><strong data-start=\"202\" data-end=\"215\">SibEnzyme<\/strong> announces the commercial availability of the restriction endonuclease <strong data-start=\"286\" data-end=\"294\">EmiI<\/strong>.<\/p>\n<p data-start=\"386\" data-end=\"731\">EmiI is a <strong data-start=\"396\" data-end=\"433\">Type IIS restriction endonuclease<\/strong> that recognizes the non-palindromic DNA sequence<br data-start=\"482\" data-end=\"485\" \/><strong data-start=\"485\" data-end=\"501\">5\u2032-CTCTTC-3\u2032<\/strong> and cleaves DNA outside the recognition site at positions <strong data-start=\"560\" data-end=\"567\">1\/4<\/strong>, generating <strong data-start=\"580\" data-end=\"613\">three-nucleotide 5\u2032 overhangs<\/strong>.<\/p>\n<p data-start=\"386\" data-end=\"731\">The enzyme was isolated from\u00a0<em data-start=\"325\" data-end=\"352\">Exiguobacterium mexicanum<\/em>\u00a06 and\u00a0<a href=\"https:\/\/dnape.online\/en\/2025\/12\/emi-i\/\" target=\"_blank\" rel=\"noopener\">thoroughly characterized<\/a>. EmiI is an isoschizomer of <strong data-start=\"642\" data-end=\"653\">Ksp632I<\/strong> and <strong data-start=\"658\" data-end=\"667\">Bst6I<\/strong> and exhibits high activity at <strong data-start=\"698\" data-end=\"707\">37 \u00b0C<\/strong> in standard SE buffers.<\/p>\n<p data-start=\"733\" data-end=\"858\">The enzyme is well suited for applications in <strong data-start=\"779\" data-end=\"857\">molecular cloning, genetic engineering, biotechnology, and DNA diagnostics<\/strong>.<\/p>\n<p data-start=\"860\" data-end=\"943\">EmiI is <a href=\"https:\/\/sibenzyme.com\/product\/emi-i\/\">available for order<\/a> as a purified enzyme preparation<\/p>\n<p>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;4799&#8243; img_size=&#8221;large&#8221;][\/vc_column][\/vc_row]<\/p>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text] SibEnzyme announces the commercial availability of the restriction endonuclease EmiI. EmiI is a Type IIS restriction endonuclease that recognizes the non-palindromic DNA sequence5\u2032-CTCTTC-3\u2032 and cleaves DNA outside the recognition site at positions 1\/4, generating three-nucleotide 5\u2032 overhangs. The enzyme was isolated from\u00a0Exiguobacterium mexicanum\u00a06 and\u00a0thoroughly characterized. EmiI is an isoschizomer of Ksp632I and Bst6I [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[923,85],"tags":[1702],"class_list":["post-4798","post","type-post","status-publish","format-standard","hentry","category-english","category-novosti","tag-post_tag_b1f7a0"],"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts\/4798","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/comments?post=4798"}],"version-history":[{"count":2,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts\/4798\/revisions"}],"predecessor-version":[{"id":4810,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts\/4798\/revisions\/4810"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=4798"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/categories?post=4798"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/tags?post=4798"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}