{"id":5344,"date":"2026-10-29T18:05:57","date_gmt":"2026-10-29T11:05:57","guid":{"rendered":"https:\/\/sibenzyme.com\/?p=5344"},"modified":"2026-10-29T18:17:07","modified_gmt":"2026-10-29T11:17:07","slug":"seg-i","status":"publish","type":"post","link":"https:\/\/sibenzyme.com\/en\/2026\/seg-i\/","title":{"rendered":"New Restriction Endonuclease SegI Now Available from SibEnzyme"},"content":{"rendered":"<div class=\"wpb-content-wrapper\" id=\"wpb-content-root\"><p>[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text css=&#8221;&#8221;]SibEnzyme announces the commercial availability of <a href=\"https:\/\/sibenzyme.com\/en\/se-rebase\/?rebase_view=all&amp;enzyme=seg-i\" target=\"_blank\" rel=\"noopener\"><strong>SegI<\/strong><\/a>, a new rare-cutting Type II restriction endonuclease isolated from <em>Sphingobium erigeronicola<\/em> 46.<\/p>\n<p>SegI recognizes the degenerate palindromic octanucleotide sequence <strong>5\u2032-RTGC\u2193GCAY-3\u2032<\/strong>, where R = A or G and Y = C or T, and cleaves both DNA strands at the center of the recognition site, generating blunt ends.<\/p>\n<p>The enzyme is an isoschizomer of FspAI and provides an additional option for applications requiring the cleavage of DNA at specific 8-bp recognition sequences.<\/p>\n<h3>Key Features<\/h3>\n<ul>\n<li><strong>Recognition site:<\/strong> 5\u2032-RTGC\u2193GCAY-3\u2032<\/li>\n<li><strong>Cleavage pattern:<\/strong> Blunt ends<\/li>\n<li><strong>Isoschizomer:<\/strong> FspAI<\/li>\n<li><strong>Optimal reaction temperature:<\/strong> 37\u00b0C<\/li>\n<li><strong>Recommended reaction buffer:<\/strong> SE buffer W<\/li>\n<li><strong>Enzyme activity:<\/strong> 5,000 U\/mL<\/li>\n<\/ul>\n<p>SegI is suitable for applications requiring large DNA fragments, including molecular cloning, DNA mapping, genetic engineering, and other molecular biology workflows.<\/p>\n<p>SegI was developed and characterized by researchers at SibEnzyme. The results of this study, including the determination of the recognition sequence, cleavage position, and optimal reaction conditions, are presented in the following scientific publication: Gonchar D.A., Chernukhin V.A., Abdurashitov M.A., Dedkov V.S., Mikhnenkova N.A., Belichenko O.A., Degtyarev S.Kh. (2026). <a href=\"https:\/\/dnape.online\/en\/2026\/10\/seg-i\/\" target=\"_blank\" rel=\"noopener\">SegI restriction endonuclease from <em>Sphingobium erigeronicola<\/em> 46 recognizes the octanucleotide DNA sequence 5\u2032-RTGC\u2193GCAY-3\u2032 and is an isoschizomer of FspAI<\/a>. <em>DNA-Recognizing Enzymes<\/em>, 2026(5).<\/p>\n<p><strong>SegI is now <a href=\"https:\/\/sibenzyme.com\/product\/emis6-i-kopirovat\/\">commercially available<\/a> from SibEnzyme.<\/strong> Contact our team for ordering information and product details.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;5347&#8243; img_size=&#8221;large&#8221; css=&#8221;&#8221;][vc_single_image image=&#8221;5313&#8243; img_size=&#8221;large&#8221; css=&#8221;&#8221;][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>The new rare-cutting restriction endonuclease SegI from Sphingobium erigeronicola 46 is now available for ordering. SegI recognizes the sequence 5\u2032-RTGC\u2193GCAY-3\u2032, generates blunt ends, and is an isoschizomer of FspAI.<\/p>\n","protected":false},"author":1,"featured_media":5347,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"rs_blank_template":"","rs_page_bg_color":"","slide_template_v7":"","footnotes":""},"categories":[923,84,85],"tags":[],"class_list":["post-5344","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-english","category-katalog","category-novosti"],"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts\/5344","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/comments?post=5344"}],"version-history":[{"count":3,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts\/5344\/revisions"}],"predecessor-version":[{"id":5348,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/posts\/5344\/revisions\/5348"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/5347"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=5344"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/categories?post=5344"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/tags?post=5344"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}