{"id":1746,"date":"2022-04-04T22:03:00","date_gmt":"2022-04-04T15:03:00","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/mal-i\/"},"modified":"2026-01-11T17:57:59","modified_gmt":"2026-01-11T10:57:59","slug":"mal-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/mal-i\/","title":{"rendered":"Mal I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
G(mA)TC<\/td>\nG(mA)\u2191TC<\/td>\n<\/tr>\n
CT(mA)G<\/td>\nCT\u2193(mA)G<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Marinococus albus I
\nSubstrate specificity<\/strong>:
\nUnit definition: <\/strong>One unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of pBR322 DNA (dam-methylated) in 1 hour at 37\u00b0C in a total reaction volume of 50 \u03bcl.
\nAssayed on: <\/strong>pBR322 DNA (dam-methylated)
\nOptimal SE-buffer:<\/strong> SE-buffer MalI
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
25<\/td>\n25<\/td>\n50<\/td>\n75<\/td>\n50<\/td>\n50<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 \u03bcg\/ml BSA, 50% glycerol; Store at -20\u00b0C.
\nLigation<\/strong>: After 5-fold overdigestion with enzyme more than 20% of the DNA fragments can be ligated and recut.
\nNon-specific hydrolisis<\/strong>: No nonspecific activity was detected after incubation of 1 \u03bcg of DNA with 10 u.a. of enzyme for 16 hours at 37\u00b0C.Reagents Supplied with Enzyme<\/b><\/p>\n

10 X SE-buffer MalI
\nMethylation sensitivity<\/b>: The enzyme cleaves only methylated DNA.
\nNotes: <\/strong>References<\/b>:<\/strong>
\n0<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

MD DNA endonuclease Mal I.
The enzyme cleaves C5-methylated DNA and doesn’t cut unmodified DNA.<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[69],"product_tag":[82],"class_list":{"0":"post-1746","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-md","7":"product_tag-epigenetics","8":"pa_285b89be-pa_285b89b_2d3edf-15","9":"pa_4ebaafd1-pa_4ebaafd_d66329-383","10":"pa_50b54da1-pa_50b54da_6e490e","11":"pa_be110261-pa_be11026_383772-2","12":"pa_concentration-pa_concent_8cc665-1","13":"pa_fasovka-0284ebff72","14":"pa_fasovka-12ce35065a","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-c498b4c014","18":"pa_variant-d2203cb123","20":"first","21":"instock","22":"taxable","23":"shipping-taxable","24":"purchasable","25":"product-type-variable","26":"berocket_lgv_grid","27":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1746","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1746"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1746"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1746"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1746"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}