{"id":1755,"date":"2022-04-04T22:03:00","date_gmt":"2022-04-04T15:03:00","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/bls-i\/"},"modified":"2026-01-11T17:58:00","modified_gmt":"2026-01-11T10:58:00","slug":"bls-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/bls-i\/","title":{"rendered":"Bls I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n\n
DNA sequence with at least two 5mC: <\/td>\nDNA sequence with at least two 5mC: <\/td>\n<\/tr>\n
RYN\u2191RY<\/td>\nRYN\u2191RY<\/td>\n<\/tr>\n
YR\u2193NYR<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Bacillus simplex 23
Substrate specificity<\/strong>:
Unit definition: <\/strong>One unit is defined as the amount of enzyme
required to hydrolyze at least one of three canonical sites
5`-G(5mC)NG(5mC)-3`\/3`-CGN(5mC)G-5`
in 1 μg of linearized plasmid pFsp4HI3 in 1 hour at 30°C in a total reaction volume of 50 μl. As a result a linearized plasmid pFsp4HI3 disappears (see run 4 in the figure). <\/p>\n\n\n\n
BlsI activity assay on DNA pFsp4HI3\/DriI <\/b>
Lanes:
1 and 7 \u2013 1 Kb SE DNA-markers.
2 \u2013 Control DNA pFsp4HI3\/DriI,
3 \u2013 0.5 μl Bls I (1\/5)
4 \u2013 1 μl Bls I (1\/5)
5 \u2013 2 μl Bls I (1\/5)
6 \u2013 1 μl of undiluted BlsI<\/p>\n

Products were separated in 1,4% agarose gel in Buffer TAE.<\/td>\n

BlsI activity assay on DNA pFsp4HI3\/DriI<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Assayed on: <\/strong>DNA pFsp4HI3\/DriI is a linearized plasmid pFsp4HI3, which carries a gene of DNA-methyltransferase M.Fsp4HI and includes three canonical sites:
5`-G(5mC)NG(5mC)-3`\/3`-CGN(5mC)G-5` [2]<\/a>.
Optimal SE-buffer:<\/strong> SE-buffer W
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
10<\/td>\n10<\/td>\n50<\/td>\n100<\/td>\n75<\/td>\n50<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg \/ml BSA; 50% glycerol; Store at -20°C.
Ligation<\/strong>: –
Non-specific hydrolisis<\/strong>: No detectable degradation of 1μg of Lambda DNA was observed after incubation with 5 units of enzyme for 16 hours at 30°C in a total reaction volume of 50 μl.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer W
Methylation sensitivity<\/b>: The enzyme cleaves C5-methylated DNA and doesn’t cut unmodified DNA [1]<\/a>.
Notes: <\/strong>References<\/b>:<\/strong>
1. Chernukhin V.A., Tomilova J.E., Chmuzh E.V., Sokolova O.O., Dedkov V.S., Degtyarev S.Kh.<\/b><\/i> Bacterial strain Bacillus simplex – producer of BlsI site specific endonuclease.<\/b><\/font> \/\/ Russian Federation patent RU 2322494 C1 (2006).
2. Chmuzh E.V., Kashirina Yu.G., Tomilova Yu.E., Chernukhin V.A., Okhapkina S.S., Gonchar D. A., Dedkov V.S., Abdurashitov M. A., Degtyarev S. Kh.<\/b><\/i> Gene cloning, comparative analysis of the protein structures from Fsp4HI restriction-modification system and biochemical characterization of the recombinant DNA methyltransferase M.Fsp4HI.<\/b><\/font> \/\/ Molecular Biology, V.41, No 1, p. 43-50 (2007)
Application:<\/b>
Degtyarev S.Kh., Prihod’ko G.G., Rechkunova N.I. Substrate specificity determination of restriction endonuclease SfeI.\/\/ Bioorg. chem (Moscow), Vol.14, No 6, 848-849 (1988). (In Russian)
Degtyarev S.Kh., Repin V.E., Rechkunova N.I. Proteus vulgaris – a strain producer of restriction endonuclease PvuII.\/\/ Soviet Union patent SU 1632974 (1991). (In Russian)
Kolykhalov A.A., Repin V.E., Fish A.M., Rechkunova N.I., Degtyarev S.Kh. Substrate specificity determination of restriction endonuclease Vha464I.\/\/ Sibirian Biological Journal, No 1, 60-61 (1991). (In Russian)
Degtyarev S. Kh., Kolyhalov A.A., Rechkunova N.I., Abdurashitov M.A. Acs I, a new restriction endonuclease from Arthrobacter citreus 310 recognizing 5'-Pu^AATTPy-3'. \/\/ Nucleic Acids Research, Vol. 20, No.14 (1992)
1. Chernukhin V.A., Tomilova J.E., Chmuzh E.V., Sokolova O.O., Dedkov V.S., Degtyarev S.Kh.<\/b><\/i> Bacterial strain Bacillus simplex – producer of BlsI site specific endonuclease.<\/b><\/font> \/\/ Russian Federation patent RU 2322494 C1 (2006).
2. Chmuzh E.V., Kashirina Yu.G., Tomilova Yu.E., Chernukhin V.A., Okhapkina S.S., Gonchar D. A., Dedkov V.S., Abdurashitov M. A., Degtyarev S. Kh.<\/b><\/i> Gene cloning, comparative analysis of the protein structures from Fsp4HI restriction-modification system and biochemical characterization of the recombinant DNA methyltransferase M.Fsp4HI.<\/b><\/font> \/\/ Molecular Biology, V.41, No 1, p. 43-50 (2007)
Application:<\/b>
V.A. Chernukhin, Yu.E. Tomilova, E.V. Chmuzh, O.O. Sokolova, V.S. Dedkov, S.Kh. Degtyarev Site-specific endonuclease Bls<\/i>I recognizes DNA sequence 5\u2019-G(5mC)N^GC-3\u2019 and cleaves it producing 3\u2019 sticky ends<\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.3, No 1, pp 28-33, 2007
Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro<\/i> and in silico<\/i><\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.3, No 4, pp 19-27, 2007
D. A. Gonchar, A. G. Akishev, S. Kh. Degtyarev BlsI- and GlaI-PCR assays \u2013 a new method r
of DNA methylation studyr
<\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.6, No 1, pp 5-12, 2010
Alexander G. Akishev, Danila A. Gonchar, Murat A. Abdurashitov and Sergey Kh. Degtyarev Epigenetic typing of human cancer cell lines by BlsI- and GlaI-PCR assays<\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.7, No 3, pp 5-16, 2011 <\/p>","protected":false},"excerpt":{"rendered":"

MD DNA endonuclease Bls I.
The enzyme cleaves C5-methylated DNA and doesn’t cut unmodified DNA.<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[69],"product_tag":[921],"class_list":{"0":"post-1755","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-md","7":"product_tag-epigenetika","8":"pa_285b89be-pa_285b89b_2d3edf-2","9":"pa_4ebaafd1-1197","10":"pa_50b54da1-pa_50b54da_6e490e-4","11":"pa_be110261-pa_be11026_383772-2","12":"pa_concentration-pa_concent_8cc665-1","13":"pa_fasovka-pa_fasovka_e7d4b7-4","14":"pa_fasovka-3a4f8ebe60","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-pa_site_aea581","18":"pa_variant-d2203cb123","20":"first","21":"instock","22":"taxable","23":"shipping-taxable","24":"purchasable","25":"product-type-variable","26":"berocket_lgv_grid","27":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1755","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1755"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1755"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1755"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1755"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}