{"id":1761,"date":"2022-04-04T22:03:00","date_gmt":"2022-04-04T15:03:00","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/pcs-i\/"},"modified":"2026-01-11T17:58:00","modified_gmt":"2026-01-11T10:58:00","slug":"pcs-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/pcs-i\/","title":{"rendered":"Pcs I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
(5mC)GNNNNNNN(5mC)G<\/td>\n(5mC)GNNNNN\u2191NN(5mC)G<\/td>\n<\/tr>\n
G(5mC)NNNNNNNG(5mC)<\/td>\nG(5mC)NN\u2193NNNNNG(5mC)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain that carries the cloned Pcs I gene from Paracoccus carotinifaciens 3K.
\nSubstrate specificity<\/strong>:
\nUnit definition:\u00a0<\/strong>
\nOne unit is defined as the amount of enzyme required to digest 1 \u00b5g of pMHpySE49\/DriI DNA in 1 hour at 37\u00b0C in a total reaction volume of 50 \u00b5l. As a result, a linearized plasmid pMHpySE49\/DriI disappears, and two DNA fragments are produced.<\/p>\n\n\n\n
PcsI activity assay on pMHpySE49\/DriI DNA<\/strong>
\nLanes:1 and 7 \u2013 1 Kb SE DNA-ladders
\n2 \u2013 Control pMHpySE49\/DriI
\n3 \u2013 0.25 \u03bcl Pcs I
\n4 \u2013 0.5 \u03bcl Pcs I
\n5 \u2013 1 \u03bcl Pcs I
\n6 \u2013 2 \u03bcl Pcs I
\nProducts were separated in 1,4% TAE agarose gel.<\/td>\n		\"\"<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Assayed on:<\/strong>
\npMHpySE49 plasmid DNA, linearized with DriI (pMHpySE49\/DriI).
\npMHpySE49 (3536 bp) was obtained by a ligation of the vector pMTL22\/FauNDI\/SmaI and a PCR-fragment containing M.HpySE526I (forms 5′-A(5mC)GT-3′) gene and
\nDNA sequence:
\n5′-GACGTATAAAACGTT-3′
\n3′-CTGCATATTTTGCAA-5′
\nThus, the plasmid has the optimal site of PcsI:
\n5′-A(5mC)GNNNNNNN(5mC)GT-3′
\n3′-TG(5mC)NNNNNNNG(5mC)A-5′
\nOptimal SE-buffer:<\/strong> SE-buffer 3M
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
50-75<\/td>\n50-75<\/td>\n0<\/td>\n10-25<\/td>\n50-75<\/td>\n50<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 0.1 mg\/ml BSA, 50% glycerol; Store at -20\u00b0C.
\n<\/mark>Ligation<\/strong>: –
\nNon-specific hydrolisis<\/strong>: No detectable degradation of 1\u03bcg of Lambda DNA was observed after incubation with 1 units of enzyme for 16 hours at 37\u00b0C in a total reaction volume of 50 \u03bcl.
\nReagents Supplied with Enzyme<\/b>
\n10 X SE-buffer 3M
\nMethylation sensitivity<\/b>: The enzyme cleaves only C5-methylated DNA and does not cut unmodified DNA [1]<\/a>.
\nNotes: <\/strong>When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion.
\nReferences<\/b>:<\/strong>
\n1. Chernukhin V.A., Nayakshina T.N., Tarasova M.V., Golikova L.N., Akishev A.G., Dedkov V.S., Mikhnenkova N.A., Degtyarev S.Kh.<\/i><\/b> Bacterial strain Paracoccus carotinifaciens 3K- producer of PcsI site specific endonuclease.<\/b><\/span> \/\/ Russian Federation patent RU 2377294 C1 (2009).
\n<\/p>","protected":false},"excerpt":{"rendered":"

MD DNA endonuclease Pcs I.
The enzyme cleaves C5-methylated DNA and doesn’t cut unmodified DNA.<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[69],"product_tag":[921],"class_list":{"0":"post-1761","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-md","7":"product_tag-epigenetika","8":"pa_285b89be-pa_285b89b_2d3edf-17","9":"pa_4ebaafd1-pa_4ebaafd_d66329-389","10":"pa_50b54da1-pa_50b54da_6e490e","11":"pa_be110261-pa_be11026_383772-2","12":"pa_concentration-pa_concent_8cc665-1","13":"pa_fasovka-0284ebff72","14":"pa_fasovka-12ce35065a","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-2860c6238e","18":"pa_variant-d2203cb123","20":"first","21":"instock","22":"featured","23":"taxable","24":"shipping-taxable","25":"purchasable","26":"product-type-variable","27":"berocket_lgv_grid","28":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1761","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1761"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1761"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1761"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1761"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}