{"id":1767,"date":"2022-04-04T22:03:00","date_gmt":"2022-04-04T15:03:00","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/aox-i\/"},"modified":"2026-01-11T17:58:00","modified_gmt":"2026-01-11T10:58:00","slug":"aox-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/aox-i\/","title":{"rendered":"Aox I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
RG(5mC)Y<\/td>\n\u2191RG(5mC)Y<\/td>\n<\/tr>\n
Y(5mC)GR<\/td>\nY(5mC)GR\u2193<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Arthrobacter oxydans 25K
Substrate specificity<\/strong>:
Unit definition: <\/strong>One unit is defined as the amount of enzyme
required to hydrolyze in 1 μg of linearized plasmid pMHaeIII\/DriI in 1 hour at 60°C in a total reaction volume of 50 μl. As a result a linearized plasmid pMHaeIII\/DriI disappears (see run 4 in the figure). <\/p>\n\n\n\n
AoxI activity assay on DNA pMHaeIII\/DriI <\/b>
Lanes:
1 \u2013 Control DNA pMHaeIII\/DriI,
2 \u2013 0.5 μl Aox I
3 \u2013 1 μl Aox I
4 \u2013 2 μl Aox I
5 \u2013 1 Kb SE DNA-markers<\/p>\n

Products were separated in 1% agarose gel in Buffer TAE.<\/td>\n

AoxI activity assay on DNA pMHaeIII\/DriI<\/center><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Assayed on: <\/strong>DNA pMHaeIII\/DriI is a linearized plasmid pMHaeIII. pMHaeIII carries a gene of DNA-methyltransferase M.HaeIII, which methylates sites 5`-GGCC-3` producing 5`-GG(5mC)C-3`\/3`-C(5mC)GG-5`.
Optimal SE-buffer:<\/strong> SE-buffer AoxI
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
75<\/td>\n25<\/td>\n10<\/td>\n25<\/td>\n75<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.4); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethan. Store at -20°C.
Ligation<\/strong>: –
Non-specific hydrolisis<\/strong>: No detectable degradation of 1μg of Lambda DNA was observed after incubation with 1 units of enzyme for 16 hours at 60°C in a total reaction volume of 50 μl.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer AoxI
Methylation sensitivity<\/b>: The enzyme cleaves C5-methylated DNA and doesn’t cut unmodified DNA.
Notes: <\/strong>References<\/b>:<\/strong>
1. Chernukhin V.A., Belichenko O.A., Tarasova M.V., Gonchar D.A., Akishev A.G., Dedkov V.S., Mikhnenkova N.A., Degtyarev S.Kh. <\/b><\/i> Bacterial strain Arthrobacter oxydans – producer of AoxI site specific endonuclease.<\/b><\/font> \/\/ Russian Federation patent RU 2399663 C1 (2009).<\/p>","protected":false},"excerpt":{"rendered":"

MD DNA endonuclease Aox I.
The enzyme cleaves C5-methylated DNA and doesn’t cut unmodified DNA.<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[69],"product_tag":[921],"class_list":{"0":"post-1767","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-md","7":"product_tag-epigenetika","8":"pa_285b89be-pa_285b89b_2d3edf-7","9":"pa_4ebaafd1-f3f0efa1e7","10":"pa_50b54da1-pa_50b54da_6e490e-3","11":"pa_be110261-pa_be11026_383772","12":"pa_concentration-pa_concent_8cc665-11","13":"pa_fasovka-pa_fasovka_e7d4b7-6","14":"pa_fasovka-pa_fasovka_e7d4b7-7","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-f0dcd25170","18":"pa_variant-d2203cb123","20":"first","21":"instock","22":"taxable","23":"shipping-taxable","24":"purchasable","25":"product-type-variable","26":"berocket_lgv_grid","27":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1767","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1767"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1767"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1767"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1767"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}