{"id":1773,"date":"2022-04-04T22:03:00","date_gmt":"2022-04-04T15:03:00","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/mte-i\/"},"modified":"2026-01-11T17:37:14","modified_gmt":"2026-01-11T10:37:14","slug":"mte-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/mte-i\/","title":{"rendered":"Mte I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
G(5mC)G(5mC)NG(5mC)G(5mC)<\/td>\nG(5mC)G(5mC)\u2191NG(5mC)G(5mC)<\/td>\n<\/tr>\n
(5mC)G(5mC)GN(5mC)G(5mC)G<\/td>\n(5mC)G(5mC)GN\u2193(5mC)G(5mC)G<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain that carries the cloned Mte I gene from Microbacterium testaceum 17B
Substrate specificity<\/strong>:
Unit definition: <\/strong>One unit is defined as the amount of enzyme required to hydrolyze completely 1 \u03bcg of linearized plasmid pHspAI10\/DriI+M.Fsp4HI in 1 hour at 55\u00b0C in a total reaction volume of 50 \u03bcl.
Assayed on: <\/strong>pHspAI10\/DriI\/metM.Fsp4HI is a plasmid pHspAI10, which is linearized with DriI, and, additionally, modified with Fsp4HI DNA methyltransferase. pHspAI10 carries a gene of HspAI DNA methyltransferase, that modifies the sequence 5`-GCGC-3`, producing 5`-G(5mC)GC-3`.
M.Fsp4HI modifies the sequence 5`-GCNGC-3`, producing 5`-G(5mC)NGC-3`.
A substrate pHspAI10\/DriI additionally methylated with M.Fsp4HI includes one site 5`-G(5mC)G(5mC)NG(5mC)G(5mC)-3`\/3`-(5mC)G(5mC)GN(5mC)G(5mC)G-5`, which is MteI canonical site [1]<\/a>. The enzyme activity depends on a number and positions of methylated nucleotides in the recognition sequence. For example, MteI cuts the recognition site with six 5-methylcytosines, but the enzyme activity is reduced for more that one order [1]<\/a>.
Optimal SE-buffer:<\/strong> SE-buffer W
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
25<\/td>\n75<\/td>\n75<\/td>\n100<\/td>\n50<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 \u03bcg \/ml BSA; 50% glycerol; Store at -20\u00b0C.
Ligation<\/strong>: –
Non-specific hydrolisis<\/strong>: No detectable degradation of 1\u03bcg of Lambda DNA was observed after incubation with 40 units of enzyme for 16 hours at 55\u00b0C in a total reaction volume of 50 \u03bcl.
Reagents Supplied with Enzyme<\/b>
10 X SE-buffer W, DNA pHspAI10\/DriI additionally methylated with M.Fsp4HI.
Methylation sensitivity<\/b>: The enzyme cleaves C5-methylated DNA and doesn’t cut unmodified DNA.
Notes: <\/strong>References<\/b>:<\/strong>
1.Degtyarev S.Kh., Rechkunova N.I., Zernov Yu.P., Dedkov V.S., Chizikov V.E., Van Calligan M., Williams R., Murray E. Bsp24I, a new unusual restriction endonuclease. \/\/ Gene, No.131, 93-95 (1993).
 
1.V.A. Chernukhin, E.V. Kileva, V.A. Sokolova., D.A. Gonchar, L.N. Golikova, V.S. Dedkov, N.A. Mikhnenkova, S.Kh. Degtyarev A new methyl-directed site-specific DNA endonuclease MteI cleaves nine nucleotides sequencer
5\u2019-G(5mC)G(5mC)^NG(5mC)GC-3\u2019\/3\u2019-CG(5mC)GN^(5mC)G(5mC)G-5\u2019r
<\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.8, No 1, pp 16-26, 2012
<\/p>","protected":false},"excerpt":{"rendered":"

MD DNA endonuclease Mte I.
The enzyme cleaves C5-methylated DNA and doesn’t cut unmodified DNA.<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[69],"product_tag":[921],"class_list":{"0":"post-1773","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-md","7":"product_tag-epigenetika","8":"pa_285b89be-pa_285b89b_2d3edf-2","9":"pa_4ebaafd1-pa_4ebaafd_d66329-387","10":"pa_50b54da1-pa_50b54da_6e490e-2","11":"pa_be110261-pa_be11026_383772","12":"pa_concentration-pa_concent_8cc665","13":"pa_fasovka-0284ebff72","14":"pa_fasovka-pa_fasovka_e7d4b7-5","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-da338dc9ab","18":"pa_variant-d2203cb123","20":"first","21":"instock","22":"taxable","23":"shipping-taxable","24":"purchasable","25":"product-type-variable","26":"berocket_lgv_grid","27":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1773","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1773"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1773"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1773"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1773"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}