{"id":1776,"date":"2022-04-04T23:26:42","date_gmt":"2022-04-04T16:26:42","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/aat-ii\/"},"modified":"2026-06-22T18:54:48","modified_gmt":"2026-06-22T11:54:48","slug":"aat-ii","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/aat-ii\/","title":{"rendered":"Aat II"},"content":{"rendered":"

Enzyme name<\/th>\n Aat II<\/td>\n <\/tr>\n \n \n Prototype<\/th>\n AatII<\/td>\n <\/tr>\n \n \n Known analogs<\/th>\n Zra I<\/a><\/td>\n <\/tr>\n \n \n SKU<\/th>\n SE-E287<\/td>\n <\/tr>\n \n \n Turbo version<\/th>\n Available<\/td>\n <\/tr>\n \n \n High-concentration version<\/th>\n Not available<\/td>\n <\/tr>\n \n \n Restriction type<\/th>\n Type IIP<\/span>iType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.<\/span><\/span><\/span><\/td>\n <\/tr>\n \n \n Recognition site<\/th>\n

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5'\u2026 GACGT\u25bc<\/sup>C \u20263'<\/div>\n
3'\u2026 C\u25b2<\/sub>TGCAG \u20265'<\/div>\n <\/div>\n <\/td>\n <\/tr>\n \n \n Source<\/th>\n An E.coli strain, that carries the cloned Aat II gene from Acetobacter aceti<\/td>\n <\/tr>\n \n \n Optimal buffer<\/th>\n SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)<\/td>\n <\/tr>\n \n \n Optimal temperature<\/th>\n 37 \u00b0C<\/td>\n <\/tr>\n \n \n Inactivation temperature<\/th>\n 65 \u00b0C<\/td>\n <\/tr>\n \n \n Buffer activity<\/th>\n \n