{"id":1776,"date":"2022-04-04T23:26:42","date_gmt":"2022-04-04T16:26:42","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/aat-ii\/"},"modified":"2026-01-11T17:41:34","modified_gmt":"2026-01-11T10:41:34","slug":"aat-ii","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/aat-ii\/","title":{"rendered":"Aat II"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
GACGTC<\/td>\nGACGT\u2191C<\/td>\n<\/tr>\n
CTGCAG<\/td>\nC\u2193TGCAG<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain, that carries the cloned Aat II gene from Acetobacter aceti
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
Lambda DNA
Optimal SE-buffer:<\/strong> SE-buffer Y
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
10<\/td>\n25<\/td>\n10<\/td>\n25<\/td>\n100<\/td>\n50<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 200 μg\/ml BSA; 1 mM DTT; and 50% glycerol. Store at -20°C.
Ligations<\/strong>:
After 10-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer Y
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: not tested
Notes:<\/strong> High enzyme concentration may result in star activity.
References:<\/strong>
Sugisaki H., Maekawa Y., Kanazawa S., Takanami M. Nucleic Acid Res. 10:5747-5752(1982)<\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Aat II<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-1776","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf","8":"pa_4ebaafd1-pa_4ebaafd_d66329-366","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665","12":"pa_fasovka-pa_fasovka_e7d4b7","13":"pa_fasovka-pa_fasovka_e7d4b7-1","14":"pa_fasovka-934","15":"pa_fasovka-3a4f8ebe60","16":"pa_obem-f616284ee0","17":"pa_obem-ded9fe5589","18":"pa_package-d20caec3b4","19":"pa_package-5dbc98dcc9","20":"pa_site-db02d8b024","21":"pa_variant-d2203cb123","22":"pa_variant-6f53bfe04e","24":"first","25":"instock","26":"taxable","27":"shipping-taxable","28":"product-type-variable","29":"berocket_lgv_grid","30":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1776","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1776"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1776"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1776"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1776"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}