{"id":1788,"date":"2022-04-04T23:26:42","date_gmt":"2022-04-04T16:26:42","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/acc65-i\/"},"modified":"2026-01-11T17:37:15","modified_gmt":"2026-01-11T10:37:15","slug":"acc65-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/acc65-i\/","title":{"rendered":"Acc65 I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
GGTACC<\/td>\nG\u2191GTACC<\/td>\n<\/tr>\n
CCATGG<\/td>\nCCATG\u2193G<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Acinetobacter calcoaceticus 65
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA(dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
Lambda DNA(dcm-)
Optimal SE-buffer:<\/strong> SE-buffer W
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
10<\/td>\n25<\/td>\n75<\/td>\n100<\/td>\n10<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg\/ml BSA; 50% glycerol. Store at -20°C.
Ligations<\/strong>:
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer W
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: Blocked by overlapping Dcm methylation(Cm<\/sup>CWGG): GGTACC<\/b>WGG<\/u>.
Notes:<\/strong>
References:<\/strong>
Prichodko, G.G., Rechkunova, N.I., Repin, V.E., Degtyarev, S.Kh., Sib. Biol. J. 1: 59-60 (1991).<\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Acc65 I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-1788","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-2","8":"pa_4ebaafd1-3c6f9d3a4e","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-3","12":"pa_concentration-pa_concent_8cc665","13":"pa_fasovka-0284ebff72","14":"pa_fasovka-pa_fasovka_e7d4b7-5","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-0c9cd24f64","18":"pa_variant-d2203cb123","19":"pa_variant-6f53bfe04e","21":"first","22":"instock","23":"taxable","24":"shipping-taxable","25":"purchasable","26":"product-type-variable","27":"berocket_lgv_grid","28":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1788","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1788"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1788"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1788"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1788"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}