{"id":1802,"date":"2022-04-04T23:26:42","date_gmt":"2022-04-04T16:26:42","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/acl-i\/"},"modified":"2026-01-11T17:37:21","modified_gmt":"2026-01-11T10:37:21","slug":"acl-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/acl-i\/","title":{"rendered":"Acl I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
AACGTT<\/td>\nAA\u2191CGTT<\/td>\n<\/tr>\n
TTGCAA<\/td>\nTTGC\u2193AA<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Acinetobacter calcoaceticus
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
Lambda DNA
Optimal SE-buffer:<\/strong> SE-buffer Y + BSA
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
0<\/td>\n0<\/td>\n0<\/td>\n0<\/td>\n100<\/td>\n80<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg\/ml BSA; 0.05% Triton X-100; 50% glycerol; Store at -20°C.
Ligations<\/strong>:
After 2-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer Y, BSA
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: Blocked by CG methylation
Notes:<\/strong> To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg\/ml.
Do not use BSA for long incubation.
References:<\/strong>
Degtyarev, S.Kh., Abdurashitov, M.A., Kolyhalov, A.A., Rechkunova, N.I., Nucleic Acids Res. 20: 3787 (1992).<\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Acl I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-1802","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf","8":"pa_4ebaafd1-ff73e003d4","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-4","12":"pa_fasovka-0284ebff72","13":"pa_fasovka-12ce35065a","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-7c48bc0fad","17":"pa_variant-d2203cb123","19":"first","20":"instock","21":"taxable","22":"shipping-taxable","23":"purchasable","24":"product-type-variable","25":"berocket_lgv_grid","26":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1802","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1802"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1802"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1802"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1802"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}