{"id":1823,"date":"2022-04-04T23:27:02","date_gmt":"2022-04-04T16:27:02","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/alu-i\/"},"modified":"2026-01-11T17:37:29","modified_gmt":"2026-01-11T10:37:29","slug":"alu-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/alu-i\/","title":{"rendered":"Alu I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
AGCT<\/td>\nAG\u2191CT<\/td>\n<\/tr>\n
TCGA<\/td>\nTC\u2193GA<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An. E.coli strain that carries the cloned Alu I gene from Arthrobacter luteus
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
Lambda DNA
Optimal SE-buffer:<\/strong> SE-buffer Y
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
75<\/td>\n75<\/td>\n10<\/td>\n50<\/td>\n100<\/td>\n50<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 200 μg\/ml BSA; 1 mM DTT, 50% glycerol; Store at -20°C.
Ligations<\/strong>:
After 5-fold overdigestion with enzyme approximately 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 4 u.a. of enzyme for 16 hours at 37°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer Y.
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: not tested
Notes:<\/strong>
References:<\/strong>
Roberts, R.J., Myers, P.A., Morrison, A., Murray, K. J. Mol.Biol. 102: 157-165 (1976).
Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases<\/a> \/\/ BMC Genomics 2008, 9:305<\/a>
Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro<\/i> and in silico<\/i>.<\/a> \/\/ Translated from “Medical genetics” V.6, No 8, pp 29-36, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro<\/i> and in silico<\/i><\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006
V.A. Chernukhin, A. A. Boltengagen, G. V. Tarasova, V.S. Dedkov, S.Kh. Degtyarev New Restriction Endonuclease AluBI from Arthrobacter luteus B – AluI Isoshizomer, nonsensitive to Presence of 5-methylcytosine in the Recognition Sequence AGCT<\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.3, No 1, pp 21-27, 2007 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Alu I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-1823","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf","8":"pa_4ebaafd1-pa_4ebaafd_d66329-8","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-10","12":"pa_concentration-pa_concent_8cc665-2","13":"pa_fasovka-0284ebff72","14":"pa_fasovka-pa_fasovka_e7d4b7-8","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-40afd7e68f","18":"pa_variant-d2203cb123","19":"pa_variant-6f53bfe04e","21":"first","22":"instock","23":"taxable","24":"shipping-taxable","25":"purchasable","26":"product-type-variable","27":"berocket_lgv_grid","28":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1823","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1823"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1823"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1823"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1823"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}