{"id":1831,"date":"2022-04-04T23:27:02","date_gmt":"2022-04-04T16:27:02","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/apa-i\/"},"modified":"2026-04-28T10:57:30","modified_gmt":"2026-04-28T03:57:30","slug":"apa-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/apa-i\/","title":{"rendered":"Apa I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
GGGCCC<\/td>\nGGGCC\u2191C<\/td>\n<\/tr>\n
CCCGGG<\/td>\nC\u2193CCGGG<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain that carries the cloned Apa I gene from Acetobacter pasteurianus
\nUnit definition:<\/strong>
\nOne unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of Lambda DNA (dam-dcm-, BamHI-digest) in 1 hour at 37\u00b0C in a total reaction volume of 50 \u03bcl.
\nAssayed on:<\/strong>
\nLambda DNA\/BamHI
\nOptimal SE-buffer:<\/strong> SE-buffer Y + BSA
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
50<\/td>\n25<\/td>\n0<\/td>\n0<\/td>\n100<\/td>\n50<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 \u03bcg\/ml BSA; 50% glycerol. Store at -20\u00b0C.
\nLigations<\/strong>:
\nAfter 20-fold overdigestion with enzyme > 95% of the DNA fragments can be ligated and recut.
\nNon-specific hydrolisis<\/strong>:
\nNo nonspecific activity was detected after incubation of 1 \u03bcg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37\u00b0C.
\nReagents Supplied with Enzyme
\n<\/b>For Regular enzyme: 10 X SE-buffer Y, BSA
\nFor Turbo enzyme:\u00a0 10 X SE-buffer ROSE
\nMethylation sensitivity<\/strong>: Blocked by overlapping Dcm methylation(Cm<\/sup>CWGG): GGGCCC<\/u><\/b>WGGNotes:<\/strong> To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 \u03bcg\/ml.
\nDo not use BSA for long incubation.
\nReferences:<\/strong>
\nSeurinck, J., Van de Voorde, A., Van Montagu, M. Nucleic Acids. Res.11: 4409-4415 (1983).<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Apa I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":["post-1831","product","type-product","status-publish","has-post-thumbnail","product_cat-re","pa_285b89be-pa_285b89b_2d3edf","pa_4ebaafd1-pa_4ebaafd_d66329-368","pa_50b54da1-pa_50b54da_6e490e","pa_be110261-pa_be11026_383772-2","pa_concentration-pa_concent_8cc665","pa_fasovka-pa_fasovka_e7d4b7-22","pa_fasovka-pa_fasovka_e7d4b7-21","pa_fasovka-pa_fasovka_e7d4b7-9","pa_fasovka-pa_fasovka_e7d4b7-5","pa_obem-a4af434faa","pa_obem-843145a84e","pa_package-d20caec3b4","pa_package-5dbc98dcc9","pa_site-0f7aa397ab","pa_variant-d2203cb123","pa_variant-6f53bfe04e","first","instock","taxable","shipping-taxable","purchasable","product-type-variable","berocket_lgv_grid","berocket_lgv_list_grid"],"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1831","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1831"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1831"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1831"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1831"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}