{"id":1847,"date":"2022-04-04T23:27:42","date_gmt":"2022-04-04T16:27:42","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/asple-i\/"},"modified":"2026-01-11T17:41:35","modified_gmt":"2026-01-11T10:41:35","slug":"asple-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/asple-i\/","title":{"rendered":"AspLE I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
GCGC<\/td>\nGCG\u2191C<\/td>\n<\/tr>\n
CGCG<\/td>\nC\u2193GCG<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Arthrobacter species LE3860
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
Lambda DNA
Optimal SE-buffer:<\/strong> SE-buffer O
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
10<\/td>\n75<\/td>\n100<\/td>\n50<\/td>\n25<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg\/ml BSA; 50% glycerol. Store at -20°C.
Ligations<\/strong>:
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer O
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: Blocked by
5′-G(5mC)GC-3’\/3-CG(5mC)G-5′ methylation.
Not blocked by
5′-GCG(5mC)-3’\/3`-(5mC)GCG-5` or
5′-GCG(5mC)-3’\/3′-CGCG-5’methylation.
Cut hemimethylated site: 5′-G(5mC)GC-3’\/3′-CGCG-5′
Notes:<\/strong>
References:<\/strong>
Vysotskaya, E.M., Gonchar, D.A., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.Kh. Unpublished observations (1998).<\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease AspLE I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-1847","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-8","8":"pa_4ebaafd1-86421f78ed","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772","11":"pa_concentration-pa_concent_8cc665-3","12":"pa_fasovka-934","13":"pa_fasovka-3a4f8ebe60","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-97cf99aa99","17":"pa_variant-d2203cb123","18":"pa_variant-6f53bfe04e","20":"first","21":"instock","22":"taxable","23":"shipping-taxable","24":"purchasable","25":"product-type-variable","26":"berocket_lgv_grid","27":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1847","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1847"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1847"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1847"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1847"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}