{"id":1856,"date":"2022-04-04T23:27:42","date_gmt":"2022-04-04T16:27:42","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/asuhp-i\/"},"modified":"2026-01-11T17:37:36","modified_gmt":"2026-01-11T10:37:36","slug":"asuhp-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/asuhp-i\/","title":{"rendered":"AsuHP I"},"content":{"rendered":"

Recognition sequence<\/strong>:<\/p>\n\n\n\n\n
GGTGA(N)8<\/sub><\/td>\nGGTGA(N)8<\/sub>\u2191<\/td>\n<\/tr>\n
CCACT(N)7<\/sub><\/td>\nCCACT(N)7<\/sub>\u2193<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain that carries the cloned AsuHPI gene from Actinobacillus suis HP
Unit Definition<\/strong>: One unit is defined as the amount of enzyme required to digest 1 \u00b5g of\u00a0 \u03bb DNA in 1 hour at 37\u00b0C in a total reaction volume of 50 \u00b5l.
Optimal buffer:<\/strong> SE-buffer O
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
\u00a010-25<\/td>\n\u00a050-75<\/td>\n\u00a0100<\/td>\n\u00a075-100<\/td>\n\u00a025-50<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5), 250 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 \u00b5g\/ml BSA, 50% glycerol; Store at -20\u00b0\u0421.
Ligation<\/strong>: After 5-fold overdigestion with AsuHP I, ~30% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 \u03bcg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37\u00b0C.
Methylation sensitivity<\/strong>: Blocked by overlapping dam-methylation (GmATC): GGTGA<\/span><\/strong>TC<\/span>Reagents Supplied with Enzyme<\/strong>: 10 x SE-Buffer O
Notes: <\/strong>enzyme may cleave at N9\/N8 depending on the sequence between the ecognition and cleave sites.
References:<\/b><\/strong><\/p>\n