{"id":1921,"date":"2022-04-04T23:29:46","date_gmt":"2022-04-04T16:29:46","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/bso31-i\/"},"modified":"2026-04-28T12:17:17","modified_gmt":"2026-04-28T05:17:17","slug":"bso31-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/bso31-i\/","title":{"rendered":"Bso31 I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
GGTCTCN<\/td>\nGGTCTCN\u2191<\/td>\n<\/tr>\n
CCAGAG(N)5<\/sub><\/td>\nCCAGAG(N)5<\/sub>\u2193<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Bacillus stearothermophilus 31
\nUnit definition:<\/strong>
\nOne unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of T7 DNA in 1 hour at 55\u00b0C in a total reaction volume of 50 \u03bcl.
\nAssayed on:<\/strong>
\nT7 DNA
\nOptimal SE-buffer:<\/strong> SE-buffer O + BSA
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
25<\/td>\n75<\/td>\n100<\/td>\n75<\/td>\n25<\/td>\n40<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 \u03bcg\/ml BSA; 50% glycerol; Store at -20\u00b0C.
\nLigations<\/strong>:
\nAfter 5-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated. Of these 80% can be recut.
\nNon-specific hydrolisis<\/strong>:
\nNo nonspecific activity was detected after incubation of 1 \u03bcg of T7 DNA with 10 u.a. of enzyme for 16 hours at 55\u00b0C.Reagents Supplied with Enzyme<\/b><\/p>\n

10 X SE-buffer O, BSA
\nReagents Supplied with Enzyme<\/strong>:
\nMethylation sensitivity<\/strong>: Blocked by overlapping Dcm-methylation (CmCWGG): GAGACC<\/u><\/b>WGG
\nNot blocked by methylation GGTCT(5mC)
\nNotes:<\/strong> To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 \u03bcg\/ml.
\nDo not use BSA for long incubation.
\nReferences:<\/strong>
\nShinkarenko, N.M., Lebedeva, N.A., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1999).<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Bso31 I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-1921","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-8","8":"pa_4ebaafd1-618e47ada6","9":"pa_50b54da1-pa_50b54da_6e490e-2","10":"pa_be110261-pa_be11026_383772-3","11":"pa_concentration-494d3ec32c","12":"pa_fasovka-65253e40ca","13":"pa_fasovka-658e880e51","14":"pa_fasovka-f38c378308","15":"pa_fasovka-2bc93bb021","16":"pa_obem-5ada2cbcd7","17":"pa_obem-513434c781","18":"pa_obem-pa_obem_d1a3e6-3","19":"pa_obem-pa_obem_d1a3e6-2","20":"pa_package-d20caec3b4","21":"pa_package-5dbc98dcc9","22":"pa_site-0db07b59aa","23":"pa_variant-d2203cb123","24":"pa_variant-6f53bfe04e","26":"first","27":"instock","28":"taxable","29":"shipping-taxable","30":"purchasable","31":"product-type-variable","32":"berocket_lgv_grid","33":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1921","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1921"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1921"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1921"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1921"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}