{"id":1933,"date":"2022-04-04T23:29:46","date_gmt":"2022-04-04T16:29:46","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/bsp19-i\/"},"modified":"2026-01-11T17:37:50","modified_gmt":"2026-01-11T10:37:50","slug":"bsp19-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/bsp19-i\/","title":{"rendered":"Bsp19 I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
CCATGG<\/td>\nC\u2191CATGG<\/td>\n<\/tr>\n
GGTACC<\/td>\nGGTAC\u2193C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Bacillus species 19
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
Lambda DNA
Optimal SE-buffer:<\/strong> SE-buffer 2W + BSA
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
0<\/td>\n10<\/td>\n50<\/td>\n75<\/td>\n10<\/td>\n5<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg\/ml BSA; 50% glycerol. Store at -20°C.
Ligations<\/strong>:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer 2W, BSA
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: Bsp19I cuts hemimethylated site
5`-(5mC)CATGG-3`\/3`-GGTACC-5`
and doesn’t cut methylatedsites
5`-(5mC)CATGG-3`\/3`-GGTAC(5mC)-5` and
5`-(4mC)CATGG-3`\/3`-GGTAC(4mC)-5`.
Notes:<\/strong> To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg\/ml.
Do not use BSA for long incubation.
References:<\/strong>
Repin, V.E., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations. Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M. Biotekhnologiya 0: 18-27 (1998).
Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro<\/i> and in silico<\/i><\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.3, No 4, pp 19-27, 2007 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Bsp19 I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-1933","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-10","8":"pa_4ebaafd1-30e280c751","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665","12":"pa_fasovka-0284ebff72","13":"pa_fasovka-pa_fasovka_e7d4b7-5","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-cfd0d34ab1","17":"pa_variant-d2203cb123","18":"pa_variant-6f53bfe04e","20":"first","21":"instock","22":"taxable","23":"shipping-taxable","24":"purchasable","25":"product-type-variable","26":"berocket_lgv_grid","27":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1933","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1933"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1933"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1933"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1933"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}