{"id":1962,"date":"2022-04-04T23:30:26","date_gmt":"2022-04-04T16:30:26","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/bstap-i\/"},"modified":"2026-01-11T17:37:56","modified_gmt":"2026-01-11T10:37:56","slug":"bstap-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/bstap-i\/","title":{"rendered":"BstAP I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
GCANNNNNTGC<\/td>\nGCANNNN\u2191NTGC<\/td>\n<\/tr>\n
CGTNNNNNACG<\/td>\nCGTN\u2193NNNNACG<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Bacillus stearothermophilus AP
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
Lambda DNA
Optimal SE-buffer:<\/strong> SE-buffer W
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
25<\/td>\n25<\/td>\n75<\/td>\n100<\/td>\n25<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 1 mM DTT; 200 μg\/ml BSA; and 50% glycerol Store at -20°C.
Ligations<\/strong>:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer W
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: not tested
Notes:<\/strong> High enzyme concentration may result in star activity. At 37\u00b0C activiti is 50% from maximum.
The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,5.
References:<\/strong>
Abdurashitov, M.A., Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Nucleic Acids Res. 25: 2301-2302 (1997).<\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease BstAP I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-1962","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-2","8":"pa_4ebaafd1-1f3a81ba76","9":"pa_50b54da1-pa_50b54da_6e490e-3","10":"pa_be110261-pa_be11026_383772-3","11":"pa_concentration-pa_concent_8cc665-1","12":"pa_fasovka-0284ebff72","13":"pa_fasovka-12ce35065a","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-951f8ae7d2","17":"pa_variant-d2203cb123","19":"first","20":"instock","21":"taxable","22":"shipping-taxable","23":"purchasable","24":"product-type-variable","25":"berocket_lgv_grid","26":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1962","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1962"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1962"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1962"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1962"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}