{"id":1992,"date":"2022-04-04T23:31:14","date_gmt":"2022-04-04T16:31:14","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/bstkt-i\/"},"modified":"2026-01-11T17:38:04","modified_gmt":"2026-01-11T10:38:04","slug":"bstkt-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/bstkt-i\/","title":{"rendered":"BstKT I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
GATC<\/td>\nGAT\u2191C<\/td>\n<\/tr>\n
CTAG<\/td>\nC\u2193TAG<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Bacillus stearothermophilus KT
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
Lambda DNA (dam-)
Optimal SE-buffer:<\/strong> SE-buffer W
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
25<\/td>\n50<\/td>\n75<\/td>\n100<\/td>\n50<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg\/ml BSA; 50% glycerol. Store at -20°C.
Ligations<\/strong>:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer W
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: Blocked by overlapping Dam methylation (Gm<\/sup>ATC) GATC<\/u><\/b>.
Not blocked by CG methylation.
Cut hemimethylated site:5`- GmATC-3` \/ 5`-GATC-3`
Notes:<\/strong>
References:<\/strong>
Nadeev A.N., Chernukhin V. A., Sevastyanova O.O., Tomilova J.E., Shinkarenko N.M., Evdokimov A.A., Degtyarev S. Kh. BstKTI, a New dam-Sensitive Neoschizomer of Restriction Endonuclease MboI, which is Able to Cleave Hemimethylated Substrate.\/\/ Biotekhnologia (Moscow), No.2, 5-10 (2006). (In Russian)<\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease BstKT I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-1992","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-2","8":"pa_4ebaafd1-c0efe572eb","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-2","12":"pa_fasovka-0284ebff72","13":"pa_fasovka-12ce35065a","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-ee9792a2e3","17":"pa_variant-d2203cb123","19":"first","20":"instock","21":"taxable","22":"shipping-taxable","23":"purchasable","24":"product-type-variable","25":"berocket_lgv_grid","26":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/1992","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=1992"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=1992"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=1992"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=1992"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}