{"id":2025,"date":"2022-04-04T23:31:57","date_gmt":"2022-04-04T16:31:57","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/bstx2-i-vremenno-nedostupen\/"},"modified":"2026-01-11T17:38:10","modified_gmt":"2026-01-11T10:38:10","slug":"bstx2-i-vremenno-nedostupen","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/bstx2-i-vremenno-nedostupen\/","title":{"rendered":"BstX2 I"},"content":{"rendered":"

5Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
RGATCY<\/td>\nR\u2191GATCY<\/td>\n<\/tr>\n
YCTAGR<\/td>\nYCTAG\u2193R<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain that carries the cloned BstX2I gene from Bacillus stearothermophilus X2
\nUnit definition:<\/strong>
\nOne unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of Lambda DNA in 1 hour at 60\u00b0C in a total reaction volume of 50 \u03bcl.
\nAssayed on:<\/strong>
\nLambda DNA
\nOptimal SE-buffer:<\/strong> SE-buffer G
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
75<\/td>\n100<\/td>\n0<\/td>\n10<\/td>\n25<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 \u03bcg\/ml BSA; 50% glycerol. Store at -20\u00b0C.
\nLigations<\/strong>:
\nAfter 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
\nNon-specific hydrolisis<\/strong>:
\nNo nonspecific activity was detected after incubation of 1 \u03bcg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60\u00b0C.Reagents Supplied with Enzyme<\/b><\/p>\n

10 X SE-buffer G
\nReagents Supplied with Enzyme<\/strong>:
\nMethylation sensitivity<\/strong>: Not blocked by Dam-methylation (Gm<\/sup>ATC): RGATC<\/u>Y<\/b>Notes:<\/strong>
\nReferences:<\/strong>
\nBelichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).
\n__eng46 <\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease BstX2 I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2025","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-4","8":"pa_4ebaafd1-pa_4ebaafd_d66329-20","9":"pa_50b54da1-pa_50b54da_6e490e-3","10":"pa_be110261-pa_be11026_383772-3","11":"pa_concentration-pa_concent_8cc665-1","12":"pa_fasovka-934","13":"pa_fasovka-3a4f8ebe60","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-30fe8ca62b","17":"pa_variant-d2203cb123","19":"first","20":"instock","21":"taxable","22":"shipping-taxable","23":"purchasable","24":"product-type-variable","25":"berocket_lgv_grid","26":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2025","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2025"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2025"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2025"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2025"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}