{"id":2031,"date":"2022-04-04T23:31:57","date_gmt":"2022-04-04T16:31:57","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/bsur-i\/"},"modified":"2026-01-11T17:38:11","modified_gmt":"2026-01-11T10:38:11","slug":"bsur-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/bsur-i\/","title":{"rendered":"BsuR I"},"content":{"rendered":"<p><strong>Recognition site and hydrolysis position<\/strong>:<\/p>\n<table>\n<tbody>\n<tr>\n<td>GGCC<\/td>\n<td>GG\u2191CC<\/td>\n<\/tr>\n<tr>\n<td>CCGG<\/td>\n<td>CC\u2193GG<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>Source:<\/strong> Bacillus subtilis R<br \/><strong>Unit definition:<\/strong><br \/>One unit of the enzyme is the amount required to hydrolyze 1 &#0956;g of Lambda DNA in 1 hour at 37&#176;C in a total reaction volume of 50 &#0956;l.<br \/><strong>Assayed on:<\/strong><br \/>Lambda DNA<br \/><strong>Optimal SE-buffer:<\/strong> SE-buffer G<br \/><strong>Enzyme activity (%)<\/strong>:<\/p>\n<table>\n<tbody>\n<tr align=\"center\">\n<td>B<\/td>\n<td>G<\/td>\n<td>O<\/td>\n<td>W<\/td>\n<td>Y<\/td>\n<td>ROSE<\/td>\n<\/tr>\n<tr align=\"center\">\n<td>75<\/td>\n<td>100<\/td>\n<td>25<\/td>\n<td>50<\/td>\n<td>50<\/td>\n<td>100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 200 &#0956;g\/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20&#176;C.<br \/><strong>Ligations<\/strong>:<br \/>After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.<br \/><strong>Non-specific hydrolisis<\/strong>:<br \/>No nonspecific activity was detected after incubation of 1 &#0956;g of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37&#176;C.<\/td>\n<\/tr>\n<tr>\n<td><b>Reagents Supplied with Enzyme<\/b><\/td>\n<td>10 X SE-buffer G<br \/><strong>Reagents Supplied with Enzyme<\/strong>:<br \/><strong>Methylation sensitivity<\/strong>: not tested<br \/><strong>Notes:<\/strong> <br \/><strong>References:<\/strong><br \/>Bron, S., Murray, K., Trautner, T.A. Mol. Gen. Genet. 143: 13-23 (1975).<!-- BsuRI --><\/p>","protected":false},"excerpt":{"rendered":"<p>Restriction endonuclease BsuR I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2031","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-4","8":"pa_4ebaafd1-fce25b3379","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-3","11":"pa_concentration-pa_concent_8cc665","12":"pa_fasovka-0284ebff72","13":"pa_fasovka-pa_fasovka_e7d4b7-5","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-2e5670136e","17":"pa_variant-d2203cb123","19":"first","20":"instock","21":"taxable","22":"shipping-taxable","23":"purchasable","24":"product-type-variable","25":"berocket_lgv_grid","26":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2031","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2031"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2031"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2031"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2031"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}