{"id":2037,"date":"2022-04-04T23:32:43","date_gmt":"2022-04-04T16:32:43","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/ccin-i\/"},"modified":"2026-01-11T17:38:17","modified_gmt":"2026-01-11T10:38:17","slug":"ccin-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/ccin-i\/","title":{"rendered":"CciN I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
GCGGCCGC<\/td>\nGC\u2191GGCCGC<\/td>\n<\/tr>\n
CGCCGGCG<\/td>\nCGCCGG\u2193CG<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Curtobacterium citreus N
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
Adenovirus-2 DNA
Optimal SE-buffer:<\/strong> SE-buffer Y
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
25<\/td>\n50<\/td>\n75<\/td>\n75<\/td>\n100<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg\/ml BSA; 50% glycerol. Store at -20°C.
Ligations<\/strong>:
After 5-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of DNA Ad-2 with 10 u.a. of enzyme for 16 hours at 37°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer Y.
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: Blocked by CG methylation.
Notes:<\/strong> High enzyme concentration may result in star activity.
References:<\/strong>
Verchozina, V.A., Degtyarev, S.Kh. Gene 157: 99-100 (1995).<\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease CciN I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2037","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf","8":"pa_4ebaafd1-pa_4ebaafd_d66329-372","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-3","12":"pa_concentration-pa_concent_8cc665-1","13":"pa_fasovka-934","14":"pa_fasovka-3a4f8ebe60","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-abee9f0428","18":"pa_variant-d2203cb123","19":"pa_variant-6f53bfe04e","21":"first","22":"instock","23":"taxable","24":"shipping-taxable","25":"purchasable","26":"product-type-variable","27":"berocket_lgv_grid","28":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2037","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2037"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2037"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2037"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2037"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}