{"id":2045,"date":"2022-04-04T23:32:43","date_gmt":"2022-04-04T16:32:43","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/dra-iii\/"},"modified":"2026-01-11T17:41:43","modified_gmt":"2026-01-11T10:41:43","slug":"dra-iii","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/dra-iii\/","title":{"rendered":"Dra III"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
CACNNNGTG<\/td>\nCACNNN\u2191GTG<\/td>\n<\/tr>\n
GTGNNNCAC<\/td>\nGTG\u2193NNNCAC<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain, that carries the cloned gene Dra III from Deinococcus radiophilus
\nUnit definition:<\/strong>
\nOne unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of Lambda DNA in 1 hour at 37\u00b0C in a total reaction volume of 50 \u03bcl.
\nAssayed on:<\/strong>
\nLambda DNA
\nOptimal SE-buffer:<\/strong> SE-buffer 2K
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
25<\/td>\n50<\/td>\n75<\/td>\n75<\/td>\n50<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 \u03bcg\/ml BSA; 50% glycerol. Store at -20\u00b0C.
\nLigations<\/strong>:
\nAfter 10-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut. In the presence of 10%PEG ligation is better.
\nNon-specific hydrolisis<\/strong>:
\nNo nonspecific activity was detected after incubation of 1 \u03bcg of DNA with 5 u.a. of enzyme for 16 hours at 37\u00b0C.Reagents Supplied with Enzyme<\/b><\/p>\n

10 X SE-buffer 2K, BSA
\nReagents Supplied with Enzyme<\/strong>:
\nMethylation sensitivity<\/strong>: not tested
\nNotes:<\/strong> High enzyme concentration may result in star activity.
\nTo obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 \u03bcg\/ml.
\nDo not use BSA for long incubation.
\nReferences:<\/strong>
\nde Wit, C.M., Dekker, B.M.M., Neele, A.C., de Waard, A., FEBS Lett. 180: 219-223 (1985)Grosskopf, R., Wolf, W., Kessler, C., Nucleic Acid res. 13: 1517-1528 (1985)<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Dra III<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2045","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-9","8":"pa_4ebaafd1-pa_4ebaafd_d66329-373","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-86cc4d1eb4","12":"pa_concentration-pa_concent_8cc665-11","13":"pa_concentration-pa_concent_8cc665-1","14":"pa_fasovka-934","15":"pa_fasovka-3a4f8ebe60","16":"pa_package-d20caec3b4","17":"pa_package-5dbc98dcc9","18":"pa_site-737a17b588","19":"pa_variant-d2203cb123","20":"pa_variant-6f53bfe04e","22":"first","23":"instock","24":"taxable","25":"shipping-taxable","26":"purchasable","27":"product-type-variable","28":"berocket_lgv_grid","29":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2045","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2045"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2045"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2045"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2045"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}