{"id":2072,"date":"2022-04-04T23:33:17","date_gmt":"2022-04-04T16:33:17","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/fat-i\/"},"modified":"2026-01-11T17:40:21","modified_gmt":"2026-01-11T10:40:21","slug":"fat-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/fat-i\/","title":{"rendered":"Fat I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
CATG<\/td>\n\u2191CATG<\/td>\n<\/tr>\n
GTAC<\/td>\nGTAC\u2193<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain that carries the cloned Fat I gene from Flavobacterium aquatile NL3
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
pUC19 DNA
Optimal SE-buffer:<\/strong> SE-buffer G
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
10<\/td>\n100<\/td>\n25<\/td>\n10<\/td>\n50<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 1 mM DTT; 200 μg\/ml BSA; and 50% glycerol. Store at -20°C.
Ligations<\/strong>:
After 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of DNA with 3 u.a. of enzyme for 16 hours at 55°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer G
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: Blocked by m<\/sup>CATG methylation
Notes:<\/strong> The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,25.
FatI cleaves linear plasmid DNA at a rate 1.5-2 times higher than supercoiled plasmid DNA.
References:<\/strong>
Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Biotekhnologia #5, 3-7 (2002)
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro<\/i> and in silico<\/i><\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Fat I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2072","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-4","8":"pa_4ebaafd1-pa_4ebaafd_d66329-30","9":"pa_50b54da1-pa_50b54da_6e490e-2","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-10","12":"pa_concentration-pa_concent_8cc665-2","13":"pa_fasovka-pa_fasovka_e7d4b7-4","14":"pa_fasovka-3a4f8ebe60","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-f5073931f7","18":"pa_variant-d2203cb123","20":"first","21":"instock","22":"taxable","23":"shipping-taxable","24":"purchasable","25":"product-type-variable","26":"berocket_lgv_grid","27":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2072","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2072"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2072"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2072"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2072"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}