{"id":2075,"date":"2022-04-04T23:33:17","date_gmt":"2022-04-04T16:33:17","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/ars-i\/"},"modified":"2026-01-11T20:16:08","modified_gmt":"2026-01-11T13:16:08","slug":"ars-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/ars-i\/","title":{"rendered":"Ars I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:
\n

\n
5'\u2026 \u25bc<\/sup>(N)8<\/sub>GACNNNNNNTTYG(N)11<\/sub>\u25bc<\/sup> \u20263'<\/div>\n
3'\u2026 \u25b2<\/sub>(N)13<\/sub>CTGNNNNNNAARC(N)6<\/sub>\u25b2<\/sub> \u20265'<\/div>\n <\/div>\n
\nSource:<\/strong> Arthrobacter species NTS
\nUnit definition:<\/strong>
\nOne unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of T7 DNA in 1 hour at 30\u00b0C in a total reaction volume of 50 \u03bcl.
\nAssayed on:<\/strong>
\nT7 DNA
\nOptimal SE-buffer:<\/strong> SE-buffer Y
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
0<\/td>\n0<\/td>\n0<\/td>\n0<\/td>\n100<\/td>\n20<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM KH2<\/sub>PO4<\/sub>(pH 7.4); 200 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 \u03bcg\/ml BSA, 50% glycerol. Store at -20\u00b0C.
\nLigations<\/strong>:
\nAfter 3-fold overdigestion with enzyme 70% of DNA fragments can be ligated. Of these 80% can be recut.
\nNon-specific hydrolisis<\/strong>:
\nNo nonspecific activity was detected after incubation of 1 \u03bcg of T7 DNA with 1 u.a. of enzyme for 16 hours at 30\u00b0C.Reagents Supplied with Enzyme<\/b><\/p>\n

10 X SE-buffer Y, BSA
\nReagents Supplied with Enzyme<\/strong>:
\nMethylation sensitivity<\/strong>: not tested
\nNotes:<\/strong> To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 \u03bcg\/ml.
\nDo not use BSA for long incubation.
\nReferences:<\/strong>
\n<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Ars I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2075","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf","8":"pa_4ebaafd1-7d893f1b83","9":"pa_50b54da1-pa_50b54da_6e490e-4","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-11","12":"pa_fasovka-pa_fasovka_e7d4b7-6","13":"pa_fasovka-pa_fasovka_e7d4b7-7","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-dc536dffee","17":"pa_variant-d2203cb123","19":"first","20":"instock","21":"taxable","22":"shipping-taxable","23":"purchasable","24":"product-type-variable","25":"berocket_lgv_grid","26":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2075","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2075"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2075"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2075"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2075"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}