{"id":2126,"date":"2022-04-04T23:34:35","date_gmt":"2022-04-04T16:34:35","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/hpa-ii\/"},"modified":"2026-01-11T17:41:49","modified_gmt":"2026-01-11T10:41:49","slug":"hpa-ii","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/hpa-ii\/","title":{"rendered":"Hpa II"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
CCGG<\/td>\nC\u2191CGG<\/td>\n<\/tr>\n
GGCC<\/td>\nGGC\u2193C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain, that carries the cloned gene Hpa II from Haemophilus parainfluenzae
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
Lambda DNA
Optimal SE-buffer:<\/strong> SE-buffer B
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
100<\/td>\n50<\/td>\n10<\/td>\n25<\/td>\n50<\/td>\n50<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 100 μg\/ml BSA; 50% glycerol; Store at -20°C.
Ligations<\/strong>:
After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer B
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: Blocked by CG methylation.
Notes:<\/strong>
References:<\/strong>
Sharp, P.A., Sugden, B., Sambrook, J. Biochemistry 12: 3055-3063 (1973).
Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro<\/i> and in silico<\/i><\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.3, No 4, pp 19-27, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro<\/i> and in silico<\/i><\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006 <\/p>","protected":false},"excerpt":{"rendered":"

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