{"id":2132,"date":"2022-04-04T23:34:35","date_gmt":"2022-04-04T16:34:35","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/kpn-i\/"},"modified":"2026-01-11T17:41:34","modified_gmt":"2026-01-11T10:41:34","slug":"kpn-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/kpn-i\/","title":{"rendered":"Kpn I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
GGTACC<\/td>\nGGTAC\u2191C<\/td>\n<\/tr>\n
CCATGG<\/td>\nC\u2193CATGG<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain that carries the cloned Kpn I gene from Klebsiella pneumonia
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
Lambda DNA
Optimal SE-buffer:<\/strong> SE-buffer B
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
100<\/td>\n25<\/td>\n25<\/td>\n25<\/td>\n75<\/td>\n50<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl(pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg\/ml BSA; 50% glycerol; Store at -20°C.
Ligations<\/strong>:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer B, BSA
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: Not blocked by overlapping Dcm methylation (Cm<\/sup>CWGG): GGTACC<\/b>WGG
Notes:<\/strong> High enzyme concentration may result in star activity.
Long incubation with BSA is not recommended due to star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg\/ml.
References:<\/strong>
Smith, D.I., Blattner, F.R., Davies, J. Nucleic Acids Res. 3: 343-353 (1976).
Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro<\/i> and in silico<\/i>.<\/a> \/\/ Translated from “Medical genetics” V.6, No 8, pp 29-36, 2007 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Kpn I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2132","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-6","8":"pa_4ebaafd1-pa_4ebaafd_d66329-52","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-3","11":"pa_concentration-pa_concent_8cc665","12":"pa_concentration-pa_concent_8cc665-20","13":"pa_fasovka-pa_fasovka_e7d4b7-10","14":"pa_fasovka-pa_fasovka_e7d4b7-8","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-ff38232ebc","18":"pa_variant-e5ef43aba2","19":"pa_variant-d2203cb123","20":"pa_variant-6f53bfe04e","22":"first","23":"instock","24":"taxable","25":"shipping-taxable","26":"purchasable","27":"product-type-variable","28":"berocket_lgv_grid","29":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2132","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2132"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2132"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2132"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2132"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}