{"id":2189,"date":"2022-04-04T23:35:52","date_gmt":"2022-04-04T16:35:52","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/pci-i\/"},"modified":"2026-01-11T17:41:50","modified_gmt":"2026-01-11T10:41:50","slug":"pci-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/pci-i\/","title":{"rendered":"Pci I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
ACATGT<\/td>\nA\u2191CATGT<\/td>\n<\/tr>\n
TGTACA<\/td>\nTGTAC\u2193A<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain that carries the cloned Pci I gene from Planococcus citreus SE-F45
\nUnit definition:<\/strong>
\nOne unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of T7 DNA in 1 hour at 37\u00b0C in a total reaction volume of 50 \u03bcl.
\nAssayed on:<\/strong>
\nT7 DNA
\nOptimal SE-buffer:<\/strong> SE-buffer O
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
50<\/td>\n75<\/td>\n100<\/td>\n75<\/td>\n50<\/td>\n50<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20\u00b0C.
\nLigations<\/strong>:
\nAfter 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
\nNon-specific hydrolisis<\/strong>:
\nNo nonspecific activity was detected after incubation of 1 \u03bcg of T7 DNA with 10 u.a. of enzyme for 16 hours at 37\u00b0C.Reagents Supplied with Enzyme<\/b>10 X SE-buffer O
\nReagents Supplied with Enzyme<\/strong>:
\nMethylation sensitivity<\/strong>: Not blocked by ACm<\/sup>ATGT methylation.
\nBlocked by m<\/sup>ACATGT methylation.
\nNotes:<\/strong> The minimum number of units that resulted in complete digestion of 1 \u03bcg of substrate DNA in 16 hours is 0,13.
\nReferences:<\/strong>
\nAbdurashitov, M.A., Nayakshina, T.N., Lebedeva, N.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (1998).
\nV.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro<\/i> and in silico<\/i><\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Pci I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2189","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-8","8":"pa_4ebaafd1-pa_4ebaafd_d66329-65","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-3","12":"pa_concentration-pa_concent_8cc665-1","13":"pa_fasovka-pa_fasovka_e7d4b7-13","14":"pa_fasovka-pa_fasovka_e7d4b7-14","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-7f8bb70158","18":"pa_variant-d2203cb123","19":"pa_variant-6f53bfe04e","21":"first","22":"instock","23":"taxable","24":"shipping-taxable","25":"purchasable","26":"product-type-variable","27":"berocket_lgv_grid","28":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2189","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2189"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2189"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2189"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2189"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}