{"id":2256,"date":"2022-04-04T23:37:16","date_gmt":"2022-04-04T16:37:16","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/sfi-i\/"},"modified":"2026-01-11T17:41:52","modified_gmt":"2026-01-11T10:41:52","slug":"sfi-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/sfi-i\/","title":{"rendered":"Sfi I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
GGCCNNNNNGGCC<\/td>\nGGCCNNNN\u2191NGGCC<\/td>\n<\/tr>\n
CCGGNNNNNCCGG<\/td>\nCCGGN\u2193NNNNCCGG<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain, that carries the cloned gene Sfi I from Streptomyces fimbriatus
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
T7 DNA
Optimal SE-buffer:<\/strong> SE-buffer G
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
75<\/td>\n100<\/td>\n25<\/td>\n25<\/td>\n25<\/td>\n75<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg\/ml BSA; 50% glycerol. Store at -20°C.
Ligations<\/strong>:
After 10-fold overdigestion with enzyme more than 70% of the DNA fragments can be ligated and recut. In the presence of 10% PEG ligation is better.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 20 u.a. of enzyme for 16 hours at 50°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer G, BSA
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: Blocked by overlapping Dcm methylation (Cm<\/sup>CWGG): GGCCWGG<\/u>NNGGCC<\/b>.
Not blocked by overlapping Dcm methylation (Cm<\/sup>CWGG): GGCCNNNNNGGCC<\/b>WGG<\/u>.
Notes:<\/strong> Incubation at 37°C rusults in 75-100% activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg\/ml.
Do not use BSA for long incubation.
References:<\/strong>
Qiang, B.-Q., Schildkraut, I. Nucleic Acids Res. 12: 4507-4515 (1984).
Dedkov V.S., Degtyarev S. Kh. The resctriction endonucleases detection in colonies of microorganisms Streptomyces<\/i> and Nocardia<\/i><\/a> \/\/ Translated from APPLIED BIOCHEMISTRY and MICROBIOLOGY (Russia) Vol 28, 1992, #. 2, 309- 313 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Sfi I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2256","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-4","8":"pa_4ebaafd1-pa_4ebaafd_d66329-391","9":"pa_50b54da1-pa_50b54da_6e490e-1","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-3","12":"pa_concentration-pa_concent_8cc665-20","13":"pa_fasovka-0284ebff72","14":"pa_fasovka-pa_fasovka_e7d4b7-5","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-3662b306db","18":"pa_variant-e5ef43aba2","19":"pa_variant-d2203cb123","20":"pa_variant-6f53bfe04e","22":"first","23":"instock","24":"taxable","25":"shipping-taxable","26":"purchasable","27":"product-type-variable","28":"berocket_lgv_grid","29":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2256","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2256"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2256"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2256"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2256"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}