{"id":2277,"date":"2022-04-04T23:37:56","date_gmt":"2022-04-04T16:37:56","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/smim-i\/"},"modified":"2026-01-11T17:39:20","modified_gmt":"2026-01-11T10:39:20","slug":"smim-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/smim-i\/","title":{"rendered":"SmiM I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
CAYNNNNRTG<\/td>\nCAYNN\u2191NNRTG<\/td>\n<\/tr>\n
GTRNNNNYAC<\/td>\nGTRNN\u2193NNYAC<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Sphingobacterium mizutae M
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
Lambda DNA
Optimal SE-buffer:<\/strong> SE-buffer W
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
10<\/td>\n10<\/td>\n75<\/td>\n100<\/td>\n10<\/td>\n60<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg\/ml BSA; and 50% glycerol. Store at -20°C.
Ligations<\/strong>:
After 5-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer W
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: not tested
Notes:<\/strong>
References:<\/strong>
Dedkov, V.S., Najakshina, T.N., Gushina, E.N., Abdurashitov, M.A., Degtyarev, S.Kh. Unpublished observations (1999).<\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease SmiM I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2277","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-2","8":"pa_4ebaafd1-b36a8c1dfa","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-1","12":"pa_fasovka-934","13":"pa_fasovka-3a4f8ebe60","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-0272ba8a35","17":"pa_variant-d2203cb123","19":"first","20":"instock","21":"taxable","22":"shipping-taxable","23":"purchasable","24":"product-type-variable","25":"berocket_lgv_grid","26":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2277","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2277"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2277"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2277"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2277"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}