{"id":2280,"date":"2022-04-04T23:37:56","date_gmt":"2022-04-04T16:37:56","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/sph-i\/"},"modified":"2026-01-11T17:39:20","modified_gmt":"2026-01-11T10:39:20","slug":"sph-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/sph-i\/","title":{"rendered":"Sph I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
GCATGC<\/td>\nGCATG\u2191C<\/td>\n<\/tr>\n
CGTACG<\/td>\nC\u2193GTACG<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain that carries the cloned Sph I gene from Streptomyces phaeochromogenes
\nUnit definition:<\/strong>
\nOne unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of Lambda DNA in 1 hour at 37\u00b0C in a total reaction volume of 50 \u03bcl.
\nAssayed on:<\/strong>
\nLambda DNA
\nOptimal SE-buffer:<\/strong> SE-buffer G
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
25<\/td>\n100<\/td>\n75<\/td>\n75<\/td>\n50<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 100 \u03bcg\/ml BSA; 50% glycerol. Store at -20\u00b0C.
\nLigations<\/strong>:
\nAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
\nNon-specific hydrolisis<\/strong>:
\nNo nonspecific activity was detected after incubation of 1 \u03bcg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37\u00b0C.Reagents Supplied with Enzyme<\/b><\/p>\n

10 X SE-buffer G, BSA
\nReagents Supplied with Enzyme<\/strong>:
\nMethylation sensitivity<\/strong>: not tested
\nNotes:<\/strong> To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 \u03bcg\/ml.
\nDo not use BSA for long incubation.
\nReferences:<\/strong>
\nFuchs, L.Y., Covarrubias, L., Escalante, L., Sanchez, S., Bolivar, F. Gene 10:39-46 (1980).<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Sph I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2280","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-4","8":"pa_4ebaafd1-pa_4ebaafd_d66329-80","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-1","12":"pa_fasovka-934","13":"pa_fasovka-3a4f8ebe60","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-69b0b73db7","17":"pa_variant-d2203cb123","18":"pa_variant-6f53bfe04e","20":"first","21":"instock","22":"taxable","23":"shipping-taxable","24":"purchasable","25":"product-type-variable","26":"berocket_lgv_grid","27":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2280","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2280"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2280"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2280"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2280"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}