{"id":2285,"date":"2022-04-04T23:38:30","date_gmt":"2022-04-04T16:38:30","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/sse9-i\/"},"modified":"2026-01-11T17:42:09","modified_gmt":"2026-01-11T10:42:09","slug":"sse9-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/sse9-i\/","title":{"rendered":"Sse9 I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
AATT<\/td>\n\u2191AATT<\/td>\n<\/tr>\n
TTAA<\/td>\nTTAA\u2193<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain that carries the cloned Sse9 I gene from Sporosarcina species
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
pBR322 DNA
Optimal SE-buffer:<\/strong> SE-buffer B
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
100<\/td>\n75<\/td>\n50<\/td>\n50<\/td>\n75<\/td>\n75<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tis-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg\/ml BSA; 50% glycerol. Store at -20°C.
Ligations<\/strong>:
After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 55°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer B, BSA
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: Blocked by methylation: 5`-A(m6A)TT-3`\/ 3`-TT(m6A)A-5`.
Notes:<\/strong> At 37°C activity is 75% from maximum.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg\/ml.
Do not use BSA for long incubation.
References:<\/strong>
Gonchar, D.A., Dedkov, V.S., Verkhozina, V.A., Kusner, Yu.S., Shevchenko, A.V., Degtyarev, S.Kh. Prikl. Biokhim. Mikrobiol. 34: 139-141 (1998).
Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro<\/i> and in silico<\/i><\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.3, No 4, pp 19-27, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro<\/i> and in silico<\/i><\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Sse9 I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2285","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-6","8":"pa_4ebaafd1-pa_4ebaafd_d66329-81","9":"pa_50b54da1-pa_50b54da_6e490e-2","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-1","12":"pa_fasovka-934","13":"pa_fasovka-3a4f8ebe60","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-4aeba9b413","17":"pa_variant-d2203cb123","18":"pa_variant-6f53bfe04e","20":"first","21":"instock","22":"taxable","23":"shipping-taxable","24":"purchasable","25":"product-type-variable","26":"berocket_lgv_grid","27":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2285","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2285"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2285"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2285"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2285"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}