{"id":2291,"date":"2022-04-04T23:38:30","date_gmt":"2022-04-04T16:38:30","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/taq-i\/"},"modified":"2026-08-05T11:02:33","modified_gmt":"2026-08-05T04:02:33","slug":"taq-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/taq-i\/","title":{"rendered":"Taq I"},"content":{"rendered":"<p>Enzyme name<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Taq I<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Prototype<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">TaqI<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">SKU<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">SE-E133<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Turbo version<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Available<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">High-concentration version<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Not available<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Restriction type<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\"><span class=\"sbe-rebase-class-item\"><span class=\"sbe-rebase-class-label\">Type IIP<\/span><span class=\"sbe-rebase-class-info\" tabindex=\"0\" aria-label=\"Type IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.\">i<span class=\"sbe-rebase-class-tooltip\">Type IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.<\/span><\/span><\/span><\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Recognition site<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\"><div class=\"rs-box\"><div class=\"rs-line\">5'\u2026 T<sup class=\"rs-cut rs-cut-top\">\u25bc<\/sup>CGA \u20263'<\/div><div class=\"rs-line\">3'\u2026 AGC<sub class=\"rs-cut rs-cut-bottom\">\u25b2<\/sub>T \u20265'<\/div><\/div><\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Source<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">An E.coli strain, that carries the cloned gene Taq I from Thermus aquaticus<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Optimal buffer<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Optimal temperature<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">65 \u00b0C<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Inactivation temperature<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">80 \u00b0C<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Buffer activity<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\"><style>\n.sbe-flat-act-wrap{\n    overflow-x:auto;\n    margin:2px 0;\n}\n.sbe-flat-act{\n    width:100%;\n    max-width:760px;\n    border-collapse:collapse;\n    table-layout:fixed;\n    font-size:14px;\n    line-height:1.25;\n    background:transparent;\n}\n.sbe-flat-act th,\n.sbe-flat-act td{\n    text-align:center;\n    padding:10px 8px;\n    border-right:1px solid #d6d6d6;\n}\n.sbe-flat-act th:last-child,\n.sbe-flat-act td:last-child{\n    border-right:none;\n}\n.sbe-flat-act thead th{\n    font-weight:700;\n    color:#1f2a44;\n}\n.sbe-flat-act tbody td{\n    color:#111;\n    background:#fff;\n    padding-top:11px;\n    padding-bottom:11px;\n    border-top:1px solid #d6d6d6;\n}\n.sbe-flat-act .buf-b{background:#8697e8;}\n.sbe-flat-act .buf-g{background:#88b68a;}\n.sbe-flat-act .buf-o{background:#efc97d;}\n.sbe-flat-act .buf-w{background:#ffffff;}\n.sbe-flat-act .buf-y{background:#e4e97f;}\n.sbe-flat-act .buf-rose{background:#e9c2cb;}\n@media (max-width:640px){\n    .sbe-flat-act{min-width:560px;}\n}\n<\/style><div class=\"sbe-flat-act-wrap\"><table class=\"sbe-flat-act\"><thead><tr><th class=\"buf-b\">B<\/th><th class=\"buf-g\">G<\/th><th class=\"buf-o\">O<\/th><th class=\"buf-w\">W<\/th><th class=\"buf-y\">Y<\/th><th class=\"buf-rose\">ROSE<\/th><\/tr><\/thead><tbody><tr><td>50<\/td><td>75<\/td><td>75<\/td><td>50<\/td><td>100<\/td><td>100<\/td><\/tr><\/tbody><\/table><\/div><\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Unit definition<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">One unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of Lambda DNA in 1 hour at 65\u00b0C in a total reaction volume of 50 \u03bcl.<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Assayed on<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Lambda DNA<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Storage conditions<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 \u03bcg\/ml BSA; 50% glycerol. Store at -20\u00b0C.<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Ligation<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">After 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Nonspecific hydrolysis<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">No nonspecific activity was detected after incubation of 1 \u03bcg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65\u00b0C.<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Methylation sensitivity<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">not tested<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Reagents supplied with enzyme<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">10 X SE-buffer Y, BSA<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Notes<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 \u03bcg\/ml. Do not use BSA for long incubation.<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">References<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">    <div class=\"sepub-list sepub-embedded\" data-sepub-language=\"en\">\n                <ol class=\"sepub-items\">\n                            <li class=\"sepub-item sepub-has-link\" data-search=\"v.a. chernukhin, m.a. abdurashitov, v.n. tomilov, d.a. gonchar, s.kh. degtyarev comparative analysis of mouse chromosomal dna digestion with restriction endonucleases in vitro and in silico\/\/ ovchinnikov bulletin of biotechnology and physical and chemical biology v.3, no 4, p. 19-27.(2007) (in russian) 2007\">\n                    <article>\n                        <div class=\"sepub-citation\">\n                                                                                    <span class=\"sepub-authors\">V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev<\/span>\n                            <span class=\"sepub-title\">\n                                                                    <a href=\"https:\/\/sibenzyme.com\/wp-content\/uploads\/se-publications\/publication-125-2007-en.pdf\"\n                                       target=\"_blank\"\n                                       rel=\"noopener noreferrer\"\n                                                                                  class=\"sepub-pdf-link\"\n                                           data-sepub-pdf=\"https:\/\/sibenzyme.com\/wp-content\/uploads\/se-publications\/publication-125-2007-en.pdf\"\n                                           data-sepub-pdf-title=\"Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico\"\n                                                                           >Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico<\/a>\n                                                                                                    <span class=\"sepub-bibliography\"> \/\/ Ovchinnikov bulletin of biotechnology and physical and chemical biology V.3, No 4, p. 19-27.(2007) (In Russian)<\/span>\n                                                            <\/span>\n                                                            <time class=\"sepub-year\" datetime=\"2007\">2007<\/time>\n                                                    <\/div>\n                                            <\/article>\n                <\/li>\n                            <li class=\"sepub-item sepub-has-link\" data-search=\"v.a. chernukhin, m.a. abdurashitov, v.n. tomilov, d.a. gonchar, s.kh. degtyarev comparative restriction enzymes analysis of rat chromosomal dna in vitro and in silico \/\/ ovchinnikov bulletin of biotechnology and physical and chemical biology , v.2, no 3, p. 39-46 (2006). (in russian) 2006\">\n                    <article>\n                        <div class=\"sepub-citation\">\n                                                                                    <span class=\"sepub-authors\">V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev<\/span>\n                            <span class=\"sepub-title\">\n                                                                    <a href=\"https:\/\/sibenzyme.com\/wp-content\/uploads\/se-publications\/publication-123-2006-en.pdf\"\n                                       target=\"_blank\"\n                                       rel=\"noopener noreferrer\"\n                                                                                  class=\"sepub-pdf-link\"\n                                           data-sepub-pdf=\"https:\/\/sibenzyme.com\/wp-content\/uploads\/se-publications\/publication-123-2006-en.pdf\"\n                                           data-sepub-pdf-title=\"Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico\"\n                                                                           >Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico<\/a>\n                                                                                                    <span class=\"sepub-bibliography\"> \/\/ Ovchinnikov bulletin of biotechnology and physical and chemical biology , V.2, No 3, p. 39-46 (2006). (In Russian)<\/span>\n                                                            <\/span>\n                                                            <time class=\"sepub-year\" datetime=\"2006\">2006<\/time>\n                                                    <\/div>\n                                            <\/article>\n                <\/li>\n                            <li class=\"sepub-item\" data-search=\"sato, s., hutchison, c.a. iii, harris, j.i. proc. natl. acad. sci. u. s. a. 74: 542-546 (1977). 1977\">\n                    <article>\n                        <div class=\"sepub-citation\">\n                                                            <span class=\"sepub-origin\">External publication<\/span>\n                                                                                    <span class=\"sepub-authors\">Sato, S., Hutchison, C.A. III, Harris, J.I.<\/span>\n                            <span class=\"sepub-title\">\n                                                                    Proc. Natl. Acad. Sci. U. S. A. 74: 542-546 (1977).                                                                                            <\/span>\n                                                            <time class=\"sepub-year\" datetime=\"1977\">1977<\/time>\n                                                    <\/div>\n                                            <\/article>\n                <\/li>\n                    <\/ol>\n        <p class=\"sepub-no-results\" hidden>Nothing found.<\/p>\n    <\/div>\n    <\/td>\n            <\/tr>\n        <\/tbody><\/table><\/div>\n<p><!-- TaqI --><strong>Recognition site and hydrolysis position<\/strong>:<\/p>\n<table>\n<tbody>\n<tr>\n<td>TCGA<\/td>\n<td>T\u2191CGA<\/td>\n<\/tr>\n<tr>\n<td>AGCT<\/td>\n<td>AGC\u2193T<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>Source:<\/strong> An E.coli strain, that carries the cloned gene Taq I from Thermus  aquaticus<br \/><strong>Unit definition:<\/strong><br \/>One unit of the enzyme is the amount required to hydrolyze 1 &#956;g of Lambda DNA in 1 hour at 65&#176;C in a total reaction volume of 50 &#956;l.<br \/><strong>Assayed on:<\/strong><br \/>Lambda DNA<br \/><strong>Optimal SE-buffer:<\/strong> SE-buffer Y<br \/><strong>Enzyme activity (%)<\/strong>:<\/p>\n<table>\n<tbody>\n<tr align=\"center\">\n<td>B<\/td>\n<td>G<\/td>\n<td>O<\/td>\n<td>W<\/td>\n<td>Y<\/td>\n<td>ROSE<\/td>\n<\/tr>\n<tr align=\"center\">\n<td>50<\/td>\n<td>75<\/td>\n<td>75<\/td>\n<td>50<\/td>\n<td>100<\/td>\n<td>100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 &#956;g\/ml BSA; 50% glycerol. Store at -20&#176;C.<br \/><strong>Ligations<\/strong>:<br \/>After 20-fold overdigestion with enzyme more than  95% of the DNA fragments can be ligated and recut.<br \/><strong>Non-specific hydrolisis<\/strong>:<br \/>No nonspecific activity was detected after incubation of  1 &#956;g of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65&#176;C.<\/td>\n<\/tr>\n<tr>\n<td><b>Reagents Supplied with Enzyme<\/b><\/td>\n<td>10 X SE-buffer Y, BSA<br \/><strong>Reagents Supplied with Enzyme<\/strong>:<br \/><strong>Methylation sensitivity<\/strong>: not tested<br \/><strong>Notes:<\/strong> To   obtain  100%   activity,  BSA   should  be   added to  the  1 x reaction  mix  to a final  concentration  of 100 &#956;g\/ml. <br \/>Do not use BSA for long incubation.<br \/><strong>References:<\/strong><br \/>Sato, S., Hutchison, C.A. III, Harris, J.I. Proc. Natl. Acad. Sci. U. S. A. 74: 542-546 (1977).<br \/>Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. <a target=\"_blank\" href=\"http:\/\/science.sibenzyme.com\/article14_article_46_1.phtml\" rel=\"noopener\">Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases <i>in vitro<\/i> and in <i>silico<\/i><\/a> \/\/ Translated from &#8220;Ovchinnikov bulletin of biotechnology and physical and chemical biology&#8221; V.3, No 4, pp 19-27, 2007 <br \/>V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev  <a target=\"_blank\" href=\"http:\/\/science.sibenzyme.com\/article14_article_28_1.phtml\" rel=\"noopener\">Comparative restriction enzymes analysis of rat chromosomal DNA <i>in vitro<\/i> and <i>in silico<\/i><\/a> \/\/ Translated from &#8220;Ovchinnikov bulletin of biotechnology and physical and chemical biology&#8221; V.2, No 3, pp 39-46, 2006  <!-- TaqI --><\/p>","protected":false},"excerpt":{"rendered":"<p>Restriction endonuclease Taq I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":["post-2291","product","type-product","status-publish","has-post-thumbnail","product_cat-re","pa_285b89be-pa_285b89b_2d3edf","pa_4ebaafd1-pa_4ebaafd_d66329-393","pa_50b54da1-pa_50b54da_6e490e-5","pa_be110261-pa_be11026_383772-3","pa_concentration-pa_concent_8cc665","pa_fasovka-pa_fasovka_e7d4b7-10","pa_fasovka-pa_fasovka_e7d4b7-8","pa_package-d20caec3b4","pa_package-5dbc98dcc9","pa_site-c6667da329","pa_variant-d2203cb123","pa_variant-6f53bfe04e","first","instock","taxable","shipping-taxable","purchasable","product-type-variable","berocket_lgv_grid","berocket_lgv_list_grid"],"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2291","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2291"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2291"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2291"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2291"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}