{"id":2294,"date":"2022-04-04T23:38:30","date_gmt":"2022-04-04T16:38:30","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/tru9-i\/"},"modified":"2026-01-11T17:41:59","modified_gmt":"2026-01-11T10:41:59","slug":"tru9-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/tru9-i\/","title":{"rendered":"Tru9 I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
TTAA<\/td>\nT\u2191TAA<\/td>\n<\/tr>\n
AATT<\/td>\nAAT\u2193T<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain that carries the cloned Tru9 I gene from Thermus ruber 9
\nUnit definition:<\/strong>
\nOne unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of Lambda DNA in 1 hour at 65\u00b0C in a total reaction volume of 50 \u03bcl.
\nAssayed on:<\/strong>
\nLambda DNA
\nOptimal SE-buffer:<\/strong> SE-buffer W
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
75<\/td>\n25<\/td>\n25<\/td>\n100<\/td>\n50<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl-(pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 \u03bcg\/ml BSA; 50% glycerol Store at -20\u00b0C.
\nLigations<\/strong>:
\nAfter 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
\nNon-specific hydrolisis<\/strong>:
\nNo nonspecific activity was detected after incubation of 1 \u03bcg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65\u00b0C.Reagents Supplied with Enzyme<\/b><\/p>\n

10 X SE-buffer W
\nReagents Supplied with Enzyme<\/strong>:
\nMethylation sensitivity<\/strong>: Blocked by TTAm<\/sup>A methylation .
\nNotes:<\/strong>
\nReferences:<\/strong>
\nPrichodko, E.A., Rechkunova, N.I., Degtyarev, S.Kh. Sib. Biol. J. 1:57-59 (1991).
\nV.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro<\/i> and in silico<\/i><\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006 <\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Tru9 I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2294","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-2","8":"pa_4ebaafd1-pa_4ebaafd_d66329-86","9":"pa_50b54da1-pa_50b54da_6e490e-5","10":"pa_be110261-pa_be11026_383772-3","11":"pa_concentration-pa_concent_8cc665-3","12":"pa_concentration-pa_concent_8cc665","13":"pa_fasovka-934","14":"pa_fasovka-3a4f8ebe60","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-cc6df72997","18":"pa_variant-d2203cb123","19":"pa_variant-6f53bfe04e","21":"first","22":"instock","23":"taxable","24":"shipping-taxable","25":"purchasable","26":"product-type-variable","27":"berocket_lgv_grid","28":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2294","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2294"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2294"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2294"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2294"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}