{"id":2303,"date":"2022-04-04T23:38:30","date_gmt":"2022-04-04T16:38:30","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/vne-i\/"},"modified":"2026-01-11T17:41:59","modified_gmt":"2026-01-11T10:41:59","slug":"vne-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/vne-i\/","title":{"rendered":"Vne I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
GTGCAC<\/td>\nG\u2191TGCAC<\/td>\n<\/tr>\n
CACGTG<\/td>\nCACGT\u2193G<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain, that carries the cloned gene VneI from Vibrio nereis 18
\nUnit definition:<\/strong>
\nOne unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of Lambda DNA in 1 hour at 37\u00b0C in a total reaction volume of 50 \u03bcl.
\nAssayed on:<\/strong>
\nLambda DNA
\nOptimal SE-buffer:<\/strong> SE-buffer O
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
10<\/td>\n25<\/td>\n100<\/td>\n25<\/td>\n25<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 \u03bcg\/ml BSA; 50% glycerol. Store at -20\u00b0C.
\nLigations<\/strong>:
\nAfter 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
\nNon-specific hydrolisis<\/strong>:
\nNo nonspecific activity was detected after incubation of 1 \u03bcg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37\u00b0C.Reagents Supplied with Enzyme<\/b><\/p>\n

10 X SE-buffer O
\nReagents Supplied with Enzyme<\/strong>:
\nMethylation sensitivity<\/strong>: not tested
\nNotes:<\/strong>
\nReferences:<\/strong>
\nDegtyarev, S.Kh., Rechkunova, N.I., Netesova, N.A., Tchigikov, V.E., Malygin, E.G., Kochkin, A.V., Mikhajlov, V.V., Rasskazov, V.A. Bioorg. Khim. 13: 422-423 (1987).<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Vne I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2303","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-8","8":"pa_4ebaafd1-9cac9622f8","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-15","12":"pa_fasovka-0284ebff72","13":"pa_fasovka-pa_fasovka_e7d4b7-5","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-5e3a15313d","17":"pa_variant-d2203cb123","18":"pa_variant-6f53bfe04e","20":"first","21":"instock","22":"taxable","23":"shipping-taxable","24":"purchasable","25":"product-type-variable","26":"berocket_lgv_grid","27":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2303","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2303"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2303"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2303"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2303"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}