{"id":2309,"date":"2022-04-04T23:38:30","date_gmt":"2022-04-04T16:38:30","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/xba-i\/"},"modified":"2026-01-11T17:39:29","modified_gmt":"2026-01-11T10:39:29","slug":"xba-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/xba-i\/","title":{"rendered":"Xba I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
TCTAGA<\/td>\nT\u2191CTAGA<\/td>\n<\/tr>\n
AGATCT<\/td>\nAGATC\u2193T<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain, that carries the cloned gene XbaI from Xanthomonas badrii
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-\/HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
Lambda DNA (dam-\/HindIII-digest)
Optimal SE-buffer:<\/strong> SE-buffer O
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
75<\/td>\n75<\/td>\n100<\/td>\n50<\/td>\n75<\/td>\n25<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.
Ligations<\/strong>:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer O, BSA (except E141m, E141T and E142T).
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: Blocked by overlapping Dam-methylation (Gm<\/sup>ATC): TCTAGA<\/b>TC<\/u>Notes:<\/strong> To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg\/ml.
Do not use BSA for long incubation.
References:<\/strong>
Zain, B,S., Roberts, R.J. J. Mol. Biol. 115: 249-255 (1977).
Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro<\/i> and in silico<\/i>.<\/a> \/\/ Translated from “Medical genetics” V.6, No 8, pp 29-36, 2007 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Xba I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":["post-2309","product","type-product","status-publish","has-post-thumbnail","product_cat-re","pa_285b89be-pa_285b89b_2d3edf-8","pa_4ebaafd1-pa_4ebaafd_d66329-397","pa_50b54da1-pa_50b54da_6e490e","pa_be110261-pa_be11026_383772-2","pa_concentration-pa_concent_8cc665","pa_concentration-pa_concent_8cc665-7","pa_fasovka-pa_fasovka_e7d4b7-10","pa_fasovka-pa_fasovka_e7d4b7-8","pa_package-d20caec3b4","pa_package-5dbc98dcc9","pa_site-962ee63da6","pa_variant-e5ef43aba2","pa_variant-d2203cb123","pa_variant-6f53bfe04e","first","instock","taxable","shipping-taxable","purchasable","product-type-variable","berocket_lgv_grid","berocket_lgv_list_grid"],"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2309","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2309"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2309"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2309"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2309"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}