{"id":2340,"date":"2022-04-04T23:39:11","date_gmt":"2022-04-04T16:39:11","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/pspx-i\/"},"modified":"2026-01-11T17:41:51","modified_gmt":"2026-01-11T10:41:51","slug":"pspx-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/pspx-i\/","title":{"rendered":"PspX I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
VCTCGAGB<\/td>\nVC\u2191TCGAGB<\/td>\n<\/tr>\n
BGAGCTCV<\/td>\nBGAGCT\u2193CV<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain that carries the cloned PspX I gene from Pseudomonas species A1-1
\nUnit definition:<\/strong>
\nOne unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of Lambda DNA (HindIII-digest) in 1 hour at 37\u00b0C in a total reaction volume of 50 \u03bcl.
\nAssayed on:<\/strong>
\nLambda DNA (HindIII-digest)
\nOptimal SE-buffer:<\/strong> SE-buffer Y
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
50<\/td>\n50<\/td>\n25<\/td>\n75<\/td>\n100<\/td>\n25<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2 -mercaptoethanol, 200 \u03bcg\/ml BSA; 50% glycerol. Store at -20\u00b0C.
\nLigations<\/strong>:
\nAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
\nNon-specific hydrolisis<\/strong>:
\nNo nonspecific activity was detected after incubation of 1 \u03bcg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37\u00b0C.Reagents Supplied with Enzyme<\/b><\/p>\n

10 X SE-buffer Y, BSA
\nReagents Supplied with Enzyme<\/strong>:
\nMethylation sensitivity<\/strong>: not tested
\nNotes:<\/strong> To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 \u03bcg\/ml.
\nDo not use BSA for long incubation.
\nReferences:<\/strong>
\nGonchar D.A., Abdurashitov M.A., Belichenko O.A., Dedkov V.S., Mezentseva N.V., Tomilova J.E., Degtyarev S.Kh. Bulletin of biotechnology and physico-chemical biology named by Yu.A.Ovchinnikov, No. 1, pp18-23 (2005)<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease PspX I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2340","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf","8":"pa_4ebaafd1-pa_4ebaafd_d66329-70","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-3","11":"pa_concentration-pa_concent_8cc665-3","12":"pa_concentration-pa_concent_8cc665-7","13":"pa_fasovka-0284ebff72","14":"pa_fasovka-12ce35065a","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-f7480e3ac2","18":"pa_variant-e5ef43aba2","19":"pa_variant-d2203cb123","20":"pa_variant-6f53bfe04e","22":"first","23":"instock","24":"taxable","25":"shipping-taxable","26":"purchasable","27":"product-type-variable","28":"berocket_lgv_grid","29":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2340","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2340"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2340"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2340"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2340"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}