{"id":2350,"date":"2022-04-04T23:39:52","date_gmt":"2022-04-04T16:39:52","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/pala-i\/"},"modified":"2026-01-11T17:39:40","modified_gmt":"2026-01-11T10:39:40","slug":"pala-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/pala-i\/","title":{"rendered":"PalA I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
GGCGCGCC<\/td>\nGG\u2191CGCGCC<\/td>\n<\/tr>\n
CCGCGCGG<\/td>\nCCGCGC\u2193GG<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E. coli<\/i> strain , that carries the cloned gene from Pseudomanas alcaligenes BS17<\/i>Unit definition:<\/strong>
\nOne unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of Lambda DNA in 1 hour at 37\u00b0C in a total reaction volume of 50 \u03bcl.
\nAssayed on:<\/strong>
\nLambda DNA
\nOptimal SE-buffer:<\/strong> SE-buffer Y
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
25<\/td>\n10<\/td>\n-10<\/td>\n-10<\/td>\n100<\/td>\n40<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 \u03bcg\/ml BSA; 50% glycerol; Store at -20\u00b0C.
\nLigations<\/strong>:
\nAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and 95% of these can be recut.
\nNon-specific hydrolisis<\/strong>:
\nNo nonspecific activity was detected after incubation of 1 \u03bcg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37\u00b0C.Reagents Supplied with Enzyme<\/b><\/p>\n

10 X SE-buffer Y
\nReagents Supplied with Enzyme<\/strong>:
\nMethylation sensitivity<\/strong>: Blocked by CG methylation.
\nNotes:<\/strong>
\nReferences:<\/strong>
\nDedkov, V.S., Belichenko, O.A., Gonchar, D.A., Akishev, A.G., Yamkovaya, T.V., Mezentseva, N.V., Tomilova, J.E., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations(2005)<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease PalA I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2350","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf","8":"pa_4ebaafd1-pa_4ebaafd_d66329-388","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-1","12":"pa_fasovka-0284ebff72","13":"pa_fasovka-12ce35065a","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-1d60d96296","17":"pa_variant-d2203cb123","19":"first","20":"instock","21":"taxable","22":"shipping-taxable","23":"purchasable","24":"product-type-variable","25":"berocket_lgv_grid","26":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2350","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2350"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2350"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2350"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2350"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}