{"id":2377,"date":"2022-04-04T23:40:36","date_gmt":"2022-04-04T16:40:36","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/set-i\/"},"modified":"2026-01-11T17:39:50","modified_gmt":"2026-01-11T10:39:50","slug":"set-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/set-i\/","title":{"rendered":"Set I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
ASST<\/td>\nASST\u2191<\/td>\n<\/tr>\n
TSSA<\/td>\n\u2193TSSA<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> An E.coli strain that carries the cloned Set I gene from Streptomyces werraensis 37
Unit definition:<\/strong>
One unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the below indicated structure in 1 hour at 55°C in a total reaction volume of 20 μl.
Assayed on:<\/strong>
Double-stranded oligonucleotide
5`-CGAGTTTATAGCT<\/b>GGGCCCAAC-3`
3`-GCTCAAATATCGA<\/b>CCCGGGTTG-5`
Optimal SE-buffer:<\/strong> SE-buffer Y
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
25<\/td>\n25<\/td>\n75<\/td>\n75<\/td>\n100<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol; Store at -20°C.
Ligations<\/strong>:
After 5-fold overdigestion with enzyme, approximately 50% of the pBR322 DNA fragments can be ligated with T4 DNA Ligase and recut.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n 10 X SE-buffer Y
Non-specific hydrolisis<\/strong>:
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: not tested
Notes:<\/strong>
References:<\/strong>
Tomilova J.E., Degtyarev S.Kh Substrate specificity of new restriction endonuclease SetI<\/a> \/\/ In press
<\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Set I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2377","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf","8":"pa_4ebaafd1-pa_4ebaafd_d66329-75","9":"pa_50b54da1-pa_50b54da_6e490e-2","10":"pa_be110261-pa_be11026_383772-3","11":"pa_concentration-pa_concent_8cc665","12":"pa_concentration-pa_concent_8cc665-1","13":"pa_fasovka-0284ebff72","14":"pa_fasovka-12ce35065a","15":"pa_package-d20caec3b4","16":"pa_package-5dbc98dcc9","17":"pa_site-ad94da62ff","18":"pa_variant-e5ef43aba2","19":"pa_variant-d2203cb123","21":"first","22":"instock","23":"taxable","24":"shipping-taxable","25":"purchasable","26":"product-type-variable","27":"berocket_lgv_grid","28":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2377","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2377"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2377"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2377"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2377"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}