{"id":2387,"date":"2022-04-04T23:40:36","date_gmt":"2022-04-04T16:40:36","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/alub-i\/"},"modified":"2026-01-11T17:39:51","modified_gmt":"2026-01-11T10:39:51","slug":"alub-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/alub-i\/","title":{"rendered":"AluB I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
AGCT<\/td>\nAG\u2191CT<\/td>\n<\/tr>\n
TCGA<\/td>\nTC\u2193GA<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Arthrobacter luteus B
Unit definition:<\/strong>
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:<\/strong>
Lambda DNA
Optimal SE-buffer:<\/strong> SE-buffer B
Enzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
100<\/td>\n75<\/td>\n10<\/td>\n10<\/td>\n75<\/td>\n60<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg\/ml BSA; 50% glycerol; Store at -20°C.
Ligations<\/strong>:
After 10-fold overdigestion with enzyme 80% of the Lambda DNA fragments can be ligated with T4 DNA Ligase and can be recut.
Non-specific hydrolisis<\/strong>:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.<\/td>\n<\/tr>\n

Reagents Supplied with Enzyme<\/b><\/td>\n10 X SE-buffer B, BSA
Reagents Supplied with Enzyme<\/strong>:
Methylation sensitivity<\/strong>: AluB I is able to cleave some methylated DNA substrates.
Notes:<\/strong> AluB I is an isoschizomer of Alu I.
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg\/ml.
Do not use BSA for long incubation.
References:<\/strong>
V.A. Chernukhin, A. A. Boltengagen, G. V. Tarasova, V.S. Dedkov, S.Kh. Degtyarev New Restriction Endonuclease AluBI from Arthrobacter luteus B – AluI Isoshizomer, nonsensitive to Presence of 5-methylcytosine in the Recognition Sequence AGCT<\/a> \/\/ Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.3, No 1, pp 21-27, 2007 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease AluB I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2387","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-6","8":"pa_4ebaafd1-2f0ab6e933","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-1","12":"pa_fasovka-0284ebff72","13":"pa_fasovka-12ce35065a","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-40afd7e68f","17":"pa_variant-d2203cb123","19":"first","20":"instock","21":"taxable","22":"shipping-taxable","23":"purchasable","24":"product-type-variable","25":"berocket_lgv_grid","26":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2387","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2387"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2387"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2387"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2387"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}