{"id":2399,"date":"2022-04-04T23:40:36","date_gmt":"2022-04-04T16:40:36","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/fai-i\/"},"modified":"2026-01-11T17:40:07","modified_gmt":"2026-01-11T10:40:07","slug":"fai-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/fai-i\/","title":{"rendered":"Fai I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
YATR<\/td>\nYA\u2191TR<\/td>\n<\/tr>\n
RTAY<\/td>\nRT\u2193AY<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Flavobacterium aquatile B15<\/p>\n

FaiI cleaves 4 expected recognition sites as well as several other sites with a weaker activity.
\nIn the case of long incubation with FaiI DNA can be digested to small oligos.<\/p>\n

Unit definition:<\/strong>
\nOne unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the above indicated structure in 1 hour at 50\u00b0C in a total reaction volume of 20 \u03bcl.
\nAssayed on:<\/strong>
\nDouble-stranded oligonucleotide
\n5`- CGAGTTCA^TAGCTGGGCCCAAC -3`
\n3`- GCTCAAGT^ATCGACCCGGGTTG -5`
\nOptimal SE-buffer:<\/strong> SE-buffer B
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
100<\/td>\n50<\/td>\n10<\/td>\n25<\/td>\n25<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 \u03bcg\/ml BSA; 50% glycerol; Store at -20\u00b0C.
\nLigations<\/strong>:
\nAfter 3-fold overdigestion with enzyme about 90% of the pUC19 DNA fragments can be ligated with DNA Ligase and recut.Reagents Supplied with Enzyme<\/b><\/p>\n

10 X SE-buffer B
\nNon-specific hydrolisis<\/strong>:
\nReagents Supplied with Enzyme<\/strong>:
\nMethylation sensitivity<\/strong>:
\nNotes:<\/strong>
\nReferences:<\/strong>
\nTarasova G.V., Chernukhin V.A., Tomilova J.E., Degtyarev S.Kh Substrate specificity of new restriction endonuclease FaiI<\/a> <\/span>\/\/ In press
\n<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Fai I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-2399","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf-6","8":"pa_4ebaafd1-630b014083","9":"pa_50b54da1-pa_50b54da_6e490e-1","10":"pa_be110261-pa_be11026_383772-3","11":"pa_concentration-pa_concent_8cc665-10","12":"pa_fasovka-pa_fasovka_e7d4b7-4","13":"pa_fasovka-3a4f8ebe60","14":"pa_package-d20caec3b4","15":"pa_package-5dbc98dcc9","16":"pa_site-f1304c6cbc","17":"pa_variant-d2203cb123","19":"first","20":"instock","21":"taxable","22":"shipping-taxable","23":"purchasable","24":"product-type-variable","25":"berocket_lgv_grid","26":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2399","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2399"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2399"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2399"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2399"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}