Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase is an RNA directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The absence of RNase H activity enhances the synthesis of long cDNAs and therefore the enzyme is recommended for preparing long cDNAs.
Application: first strand cDNA synthesis.<\/p>\n
Source:<\/strong> Isolated from E.coli strain that carries the cloned Reverse transcriptase gene<\/p>\n Unit definition:<\/strong> Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase is an RNA directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The absence of RNase H activity enhances the synthesis of long cDNAs and therefore the enzyme is recommended for preparing long cDNAs. Reaction buffer:<\/strong> SE-buffer Reverse transcriptase M-MulV<\/p>\n Storage conditions:<\/strong> 10 mM KH2<\/sub>PO4 <\/sub>(pH 7.5); 0,1 mM EDTA; 200 mM NaCl; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.<\/p>\n Quality control:<\/strong> The enzyme is purified free of contaminating endonucleases andexonucleases.<\/p>\n Notes:<\/strong> High enzyme concentration may lead to RT-PCR inhibition. In this case the enzyme preparation should be diluted in 5, 10 or 20 times with M-MuLV Reverse Transcriptase dilution Buffer (supplemented) Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase is an RNA directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The absence of RNase H activity enhances the synthesis of long cDNAs and therefore the enzyme is recommended for […]<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[498],"product_tag":[],"class_list":{"0":"post-2643","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-enzymes","7":"pa_285b89be-2610ef2daf","8":"pa_4ebaafd1-9c6532fcc7","9":"pa_50b54da1-a5bfc9e079","10":"pa_concentration-1e8d754bc9","11":"pa_fasovka-589257efd5","12":"pa_fasovka-cf16014404","13":"pa_package-d20caec3b4","14":"pa_package-5dbc98dcc9","16":"first","17":"instock","18":"taxable","19":"shipping-taxable","20":"purchasable","21":"product-type-variable","22":"berocket_lgv_grid","23":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2643","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2643"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2643"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2643"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2643"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Application: first strand cDNA synthesis.<\/p>\n
\n<\/p>","protected":false},"excerpt":{"rendered":"