{"id":2646,"date":"2022-04-11T12:05:39","date_gmt":"2022-04-11T05:05:39","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/t4-dnk-polimeraza\/"},"modified":"2026-01-26T16:29:17","modified_gmt":"2026-01-26T09:29:17","slug":"t4-dnk-polimeraza","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/t4-dnk-polimeraza\/","title":{"rendered":"T4 DNA polymerase"},"content":{"rendered":"<p>T4 DNA Polymerase catalyzes the synthesis of DNA in the 5` -&gt; 3` direction and requires the presence of template and primer. This enzyme has a 3`  -&gt; 5` exonuclease activity.<br \/>Applications: <br \/>&#8211; polishing ends;<br \/>&#8211; probe labeling using replacement synthesis.<\/p>\n<p><strong>Source:<\/strong> Isolated from E.coli strain that carries the cloned T4 DNA polymerase gene<\/p>\n<p><strong>Unit definition:<\/strong> T4 DNA Polymerase catalyzes the synthesis of DNA in the 5` -&gt; 3` direction and requires the presence of template and primer. This enzyme has a 3`  -&gt; 5` exonuclease activity.<br \/>Applications: <br \/>&#8211; polishing ends;<br \/>&#8211; probe labeling using replacement synthesis.<\/p>\n<p><strong>Reaction buffer:<\/strong> SE-buffer DNA polymerase T4<\/p>\n<p><strong>Storage conditions:<\/strong> 20 mM Tris-HCl (pH 7.5); 50 mM KCl; 10 mM 2-mercaptoethanol ; 50% glycerol.  Store at -20&#176;C.<\/p>\n<p><strong>Quality control:<\/strong> The  enzyme  is  purified  free  of  contaminating  endonucleases  andexonucleases.<br \/>\n<\/p>","protected":false},"excerpt":{"rendered":"<p>T4 DNA Polymerase catalyzes the synthesis of DNA in the 5` -&gt; 3` direction and requires the presence of template and primer. This enzyme has a 3` -&gt; 5` exonuclease activity.Applications: &#8211; polishing ends;&#8211; probe labeling using replacement synthesis. Source: Isolated from E.coli strain that carries the cloned T4 DNA polymerase gene Unit definition: T4 [&hellip;]<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[498],"product_tag":[],"class_list":{"0":"post-2646","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-enzymes","7":"pa_285b89be-956d6c9af2","8":"pa_4ebaafd1-pa_4ebaafd_d66329-62","9":"pa_50b54da1-a5bfc9e079","10":"pa_concentration-9630ea1daa","11":"pa_fasovka-65253e40ca","12":"pa_fasovka-658e880e51","13":"pa_package-d20caec3b4","14":"pa_package-5dbc98dcc9","16":"first","17":"instock","18":"taxable","19":"shipping-taxable","20":"purchasable","21":"product-type-variable","22":"berocket_lgv_grid","23":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2646","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2646"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2646"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2646"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2646"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}