T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5` phosphate and 3` hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt-end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA, or DNA\/RNA hybrids.
\nApplications:
\n– cloning of restriction fragments;
\n– joining linkers and adapters to blunt-ended DNA<\/p>\n
Source:<\/strong> Isolated from E.coli strain that carries the cloned DNA ligase gene from bacteriophage T4<\/p>\n Unit definition:<\/strong> T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5` phosphate and 3` hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt-end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA, or DNA\/RNA hybrids. Reaction buffer:<\/strong> SE-buffer DNA ligase T4<\/p>\n Storage conditions:<\/strong> 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20\u00b0C.<\/p>\n Quality control:<\/strong> The enzyme is purified free of contaminating endonucleases andexonucleases.<\/p>\n Notes:<\/strong> A precipitate may be observed in T4 DNA Ligase Buffer after defrosting. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5` phosphate and 3` hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt-end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA, or DNA\/RNA hybrids. Applications: – cloning of restriction fragments; – joining linkers […]<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[498],"product_tag":[],"class_list":{"0":"post-2659","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-enzymes","7":"pa_285b89be-498dce7fd3","8":"pa_4ebaafd1-pa_4ebaafd_d66329-400","9":"pa_50b54da1-c74d97b01e","10":"pa_concentration-f022ec202e","11":"pa_fasovka-1bf49a0f05","12":"pa_fasovka-52599f5f94","13":"pa_package-d20caec3b4","14":"pa_package-5dbc98dcc9","16":"first","17":"instock","18":"taxable","19":"shipping-taxable","20":"purchasable","21":"product-type-variable","22":"berocket_lgv_grid","23":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2659","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2659"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2659"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2659"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2659"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nApplications:
\n– cloning of restriction fragments;
\n– joining linkers and adapters to blunt-ended DNA<\/p>\n
\nBefore the first use we recommend to heat this buffer at 37\u00b0C for 10-15 min and dissolve the precipitate by shaking.
\nAlso we recommend to dispense the buffer into small aliquots and store them at -20\u00b0C.
\nAvoid defrosting the buffer more than 2-3 times. The aliquot of T4 DNA Ligase Buffer may be stored at +4\u00b0C during 7 days.<\/p>","protected":false},"excerpt":{"rendered":"